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Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome.
BMC Genomics 2020; 21(1):63BG

Abstract

BACKGROUND

As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible).

RESULTS

We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype.

CONCLUSIONS

We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.

Authors+Show Affiliations

IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.Ifremer, LEMAR UMR 6539, UBO/CNRS/IRD/Ifremer, 11 presqu'île du vivier, 29840, Argenton-en-Landunvez, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France. Ifremer, UMR 241 Écosystèmes Insulaires Océaniens, Labex Corail, Centre Ifremer du Pacifique, BP 49, 98725, Tahiti, French Polynesia.Ifremer, Laboratoire de Génétique et Pathologie des Mollusques Marins, Avenue du Mus de Loup, 17930, La Tremblade, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.MARBEC, Université de Montpellier, CNRS, IRD, Ifremer, 87 Avenue Jean Monnet, 34200, Sète, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France.IHPE, Université de Montpellier, CNRS, Ifremer, Université de Perpignan Via Domitia, Place E. Bataillon, CC080, 34095, Montpellier, France. mitta@univ-perp.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31959106

Citation

de Lorgeril, Julien, et al. "Differential Basal Expression of Immune Genes Confers Crassostrea Gigas Resistance to Pacific Oyster Mortality Syndrome." BMC Genomics, vol. 21, no. 1, 2020, p. 63.
de Lorgeril J, Petton B, Lucasson A, et al. Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome. BMC Genomics. 2020;21(1):63.
de Lorgeril, J., Petton, B., Lucasson, A., Perez, V., Stenger, P. L., Dégremont, L., ... Mitta, G. (2020). Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome. BMC Genomics, 21(1), p. 63. doi:10.1186/s12864-020-6471-x.
de Lorgeril J, et al. Differential Basal Expression of Immune Genes Confers Crassostrea Gigas Resistance to Pacific Oyster Mortality Syndrome. BMC Genomics. 2020 Jan 20;21(1):63. PubMed PMID: 31959106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome. AU - de Lorgeril,Julien, AU - Petton,Bruno, AU - Lucasson,Aude, AU - Perez,Valérie, AU - Stenger,Pierre-Louis, AU - Dégremont,Lionel, AU - Montagnani,Caroline, AU - Escoubas,Jean-Michel, AU - Haffner,Philippe, AU - Allienne,Jean-François, AU - Leroy,Marc, AU - Lagarde,Franck, AU - Vidal-Dupiol,Jérémie, AU - Gueguen,Yannick, AU - Mitta,Guillaume, Y1 - 2020/01/20/ PY - 2019/10/17/received PY - 2020/01/08/accepted PY - 2020/1/22/entrez PY - 2020/1/22/pubmed PY - 2020/1/22/medline KW - Antiviral molecular pathways KW - Invertebrate immunity KW - OsHV-1 KW - Oyster disease KW - Pacific oyster KW - Resistance SP - 63 EP - 63 JF - BMC genomics JO - BMC Genomics VL - 21 IS - 1 N2 - BACKGROUND: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). RESULTS: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. CONCLUSIONS: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/31959106/Differential_basal_expression_of_immune_genes_confers_Crassostrea_gigas_resistance_to_Pacific_oyster_mortality_syndrome L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-6471-x DB - PRIME DP - Unbound Medicine ER -