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A pathogenesis related protein GmPR08-Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to Heterodera glycines.

Abstract

Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both the GmSHMT08 and GmSNAP18 in "Peking" type resistance. Here we describe the molecular interaction between GmSHMT08 and GmSNAP18, which is potentiated by a pathogenesis-related protein GmPR08-Bet VI. Like GmSNAP18 and GmSHMT08, GmPR08-Bet VI expression was induced in response to SCN and its overexpression decreased SCN cysts by 65% in infected transgenic soybean roots. Overexpression of GmPR08-Bet VI did not have an effect on SCN resistance when the two-cytokinin binding sites in GmPR08-Bet VI were mutated, indicating a new role of GmPR08-Bet VI in SCN resistance. GmPR08-Bet VI was mapped to a QTL for resistance to SCN using different mapping populations. GmSHMT08, GmSNAP18, and GmPR08-Bet VI localize to the cytosol and plasma membrane. GmSNAP18 expression and localization hyper-accumulated at the plasma membrane and was specific to the root cells surrounding the nematode in SCN resistant soybeans. Genes encoding key components of the salicylic acid signaling pathway were induced under SCN infection. GmSNAP18 and GmPR08-Bet VI were also induced under salicylic acid and cytokinin exogenous treatments, while GmSHMT08 was induced only when the resistant GmSNAP18 was present, pointing to the presence of a molecular crosstalk between SCN resistant genes and defense genes. Expression analysis of GmSHMT08 and GmSNAP18 identified the need of a minimum expression requirement to trigger the SCN resistance reaction. These results provide insight into a new response mechanism toward plant nematode resistance involving haplotype compatibility, gene dosage, and hormone signaling.

Authors+Show Affiliations

Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996.Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.INRA, Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Université Paris-Sud, Bâtiment 630, 91405, Orsay, France.Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996.Duke University, Durham, NC, 27708.Faculty of Sciences and Technologies, University of Lorraine, 54506, Nancy, France.Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.Department of Biological Sciences, Fayetteville State University, Fayetteville, NC, 28301, USA.INRA, Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Université Paris-Sud, Bâtiment 630, 91405, Orsay, France.INRA, Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Université Paris-Sud, Bâtiment 630, 91405, Orsay, France.Department of Crop Sciences, University of Illinois, Urbana, IL, 61801, USA.INRA, Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, Université Paris-Sud, Bâtiment 630, 91405, Orsay, France.Department of Plant Sciences, University of Tennessee, Knoxville, TN, 37996.Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31960590

Citation

Lakhssassi, Naoufal, et al. "A Pathogenesis Related Protein GmPR08-Bet VI Promotes a Molecular Interaction Between the GmSHMT08 and GmSNAP18 in Resistance to Heterodera Glycines." Plant Biotechnology Journal, 2020.
Lakhssassi N, Piya S, Bekal S, et al. A pathogenesis related protein GmPR08-Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to Heterodera glycines. Plant Biotechnol J. 2020.
Lakhssassi, N., Piya, S., Bekal, S., Liu, S., Zhou, Z., Bergounioux, C., ... Meksem, K. (2020). A pathogenesis related protein GmPR08-Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to Heterodera glycines. Plant Biotechnology Journal, doi:10.1111/pbi.13343.
Lakhssassi N, et al. A Pathogenesis Related Protein GmPR08-Bet VI Promotes a Molecular Interaction Between the GmSHMT08 and GmSNAP18 in Resistance to Heterodera Glycines. Plant Biotechnol J. 2020 Jan 20; PubMed PMID: 31960590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A pathogenesis related protein GmPR08-Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to Heterodera glycines. AU - Lakhssassi,Naoufal, AU - Piya,Sarbottam, AU - Bekal,Sadia, AU - Liu,Shiming, AU - Zhou,Zhou, AU - Bergounioux,Catherine, AU - Miao,Long, AU - Meksem,Jonas, AU - Lakhssassi,Aicha, AU - Jones,Karen, AU - Kassem,My Abdelmajid, AU - Benhamed,Moussa, AU - Bendahmane,Abdelhafid, AU - Lambert,Kris, AU - Boualem,Adnane, AU - Hewezi,Tarek, AU - Meksem,Khalid, Y1 - 2020/01/20/ PY - 2020/1/22/entrez KW - SCN mechanism KW - SCN-R model KW - and cytokinins KW - gene dosage effect KW - molecular crosstalk KW - molecular trafficking KW - protein-protein interaction KW - salicylic acid JF - Plant biotechnology journal JO - Plant Biotechnol. J. N2 - Soybean cyst nematode (SCN, Heterodera glycines) is the most devastating pest affecting soybean production worldwide. SCN resistance requires both the GmSHMT08 and GmSNAP18 in "Peking" type resistance. Here we describe the molecular interaction between GmSHMT08 and GmSNAP18, which is potentiated by a pathogenesis-related protein GmPR08-Bet VI. Like GmSNAP18 and GmSHMT08, GmPR08-Bet VI expression was induced in response to SCN and its overexpression decreased SCN cysts by 65% in infected transgenic soybean roots. Overexpression of GmPR08-Bet VI did not have an effect on SCN resistance when the two-cytokinin binding sites in GmPR08-Bet VI were mutated, indicating a new role of GmPR08-Bet VI in SCN resistance. GmPR08-Bet VI was mapped to a QTL for resistance to SCN using different mapping populations. GmSHMT08, GmSNAP18, and GmPR08-Bet VI localize to the cytosol and plasma membrane. GmSNAP18 expression and localization hyper-accumulated at the plasma membrane and was specific to the root cells surrounding the nematode in SCN resistant soybeans. Genes encoding key components of the salicylic acid signaling pathway were induced under SCN infection. GmSNAP18 and GmPR08-Bet VI were also induced under salicylic acid and cytokinin exogenous treatments, while GmSHMT08 was induced only when the resistant GmSNAP18 was present, pointing to the presence of a molecular crosstalk between SCN resistant genes and defense genes. Expression analysis of GmSHMT08 and GmSNAP18 identified the need of a minimum expression requirement to trigger the SCN resistance reaction. These results provide insight into a new response mechanism toward plant nematode resistance involving haplotype compatibility, gene dosage, and hormone signaling. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/31960590/A_pathogenesis_related_protein_GmPR08-Bet_VI_promotes_a_molecular_interaction_between_the_GmSHMT08_and_GmSNAP18_in_resistance_to_Heterodera_glycines L2 - https://doi.org/10.1111/pbi.13343 DB - PRIME DP - Unbound Medicine ER -