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Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States.

Abstract

Culicoides midges vector numerous veterinary and human pathogens. Many of these diseases lack effective therapeutic treatments or vaccines to limit transmission. The only effective approach to limit disease transmission is vector control. However, current vector control for Culicoides midges is complicated by the biology of many Culicoides species and is not always effective at reducing midge populations and impacting disease transmission. The endosymbiont Wolbachia pipientis Hertig may offer an alternative control approach to limit disease transmission and affect Culicoides populations. Here the detection of Wolbachia infections in nine species of Culicoides midges is reported. Infections were detected at low densities using qPCR. Wolbachia infections were confirmed with the sequencing of a partial region of the 16S gene. Fluorescence in situ hybridization of Culicoides sonorensis Wirth and Jones adults and dissected ovaries confirm the presence of Wolbachia infections in an important vector of Bluetongue and Epizootic hemorrhagic disease viruses. The presence of Wolbachia in Culicoides populations in the United States suggests the need for further investigation of Wolbachia as a strategy to limit transmission of diseases vectored by Culicoides midges.

Authors+Show Affiliations

Department of Biological Sciences, Texas Tech University, Lubbock, TX.Department of Biological Sciences, Texas Tech University, Lubbock, TX.Department of Biological Sciences, Texas Tech University, Lubbock, TX.Department of Biological Sciences, Texas Tech University, Lubbock, TX.Department of Entomology, Texas A&M University, College Station, TX.Department of Biological Sciences, Texas Tech University, Lubbock, TX.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31961929

Citation

Covey, Hunter, et al. "Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States." Journal of Medical Entomology, 2020.
Covey H, Hall RH, Krafsur A, et al. Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States. J Med Entomol. 2020.
Covey, H., Hall, R. H., Krafsur, A., Matthews, M. L., Shults, P. T., & Brelsfoard, C. L. (2020). Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States. Journal of Medical Entomology, doi:10.1093/jme/tjaa003.
Covey H, et al. Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States. J Med Entomol. 2020 Jan 21; PubMed PMID: 31961929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cryptic Wolbachia (Rickettsiales: Rickettsiaceae) Detection and Prevalence in Culicoides (Diptera: Ceratopogonidae) Midge Populations in the United States. AU - Covey,Hunter, AU - Hall,Rafe H, AU - Krafsur,Alyssa, AU - Matthews,Megan L, AU - Shults,Phillip T, AU - Brelsfoard,Corey L, Y1 - 2020/01/21/ PY - 2019/11/19/received PY - 2020/1/22/entrez PY - 2020/1/22/pubmed PY - 2020/1/22/medline KW - Culicoides KW - Wolbachia KW - biting midge JF - Journal of medical entomology JO - J. Med. Entomol. N2 - Culicoides midges vector numerous veterinary and human pathogens. Many of these diseases lack effective therapeutic treatments or vaccines to limit transmission. The only effective approach to limit disease transmission is vector control. However, current vector control for Culicoides midges is complicated by the biology of many Culicoides species and is not always effective at reducing midge populations and impacting disease transmission. The endosymbiont Wolbachia pipientis Hertig may offer an alternative control approach to limit disease transmission and affect Culicoides populations. Here the detection of Wolbachia infections in nine species of Culicoides midges is reported. Infections were detected at low densities using qPCR. Wolbachia infections were confirmed with the sequencing of a partial region of the 16S gene. Fluorescence in situ hybridization of Culicoides sonorensis Wirth and Jones adults and dissected ovaries confirm the presence of Wolbachia infections in an important vector of Bluetongue and Epizootic hemorrhagic disease viruses. The presence of Wolbachia in Culicoides populations in the United States suggests the need for further investigation of Wolbachia as a strategy to limit transmission of diseases vectored by Culicoides midges. SN - 1938-2928 UR - https://www.unboundmedicine.com/medline/citation/31961929/Cryptic_Wolbachia_(Rickettsiales:_Rickettsiaceae)_Detection_and_Prevalence_in_Culicoides_(Diptera:_Ceratopogonidae)_Midge_Populations_in_the_United_States L2 - https://academic.oup.com/jme/article-lookup/doi/10.1093/jme/tjaa003 DB - PRIME DP - Unbound Medicine ER -