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Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears.
Acta Cytol 2020; :1-3AC

Abstract

Rapid on-site evaluation (ROSE) is one of cytopathology's "unique selling propositions." The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides.

Authors+Show Affiliations

Institute of Pathology, University of Bern, Bern, Switzerland, ekkehard.hewer@pathology.unibe.ch. Department of Diabetes, Endocrinology, Nutritional Medicine and Metabolism, Inselspital, University of Bern, Bern, Switzerland, ekkehard.hewer@pathology.unibe.ch.Institute of Pathology, University of Bern, Bern, Switzerland. Department of Diabetes, Endocrinology, Nutritional Medicine and Metabolism, Inselspital, University of Bern, Bern, Switzerland. Pathologikum, Gemeinschaftspraxis für Pathologie, Zurich, Switzerland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31962321

Citation

Hewer, Ekkehard, and Anja M. Schmitt. "Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears." Acta Cytologica, 2020, pp. 1-3.
Hewer E, Schmitt AM. Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears. Acta Cytol. 2020.
Hewer, E., & Schmitt, A. M. (2020). Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears. Acta Cytologica, pp. 1-3. doi:10.1159/000505254.
Hewer E, Schmitt AM. Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears. Acta Cytol. 2020 Jan 21;1-3. PubMed PMID: 31962321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears. AU - Hewer,Ekkehard, AU - Schmitt,Anja M, Y1 - 2020/01/21/ PY - 2019/12/05/received PY - 2019/12/07/accepted PY - 2020/1/22/entrez PY - 2020/1/22/pubmed PY - 2020/1/22/medline KW - Fine-needle aspiration KW - Rapid on-site evaluation KW - Specimen adequacy SP - 1 EP - 3 JF - Acta cytologica JO - Acta Cytol. N2 - Rapid on-site evaluation (ROSE) is one of cytopathology's "unique selling propositions." The quality, speed, and ease of handling of the staining used is a critical factor for the efficacy of the ROSE procedure. Here, we describe a modification of rapid toluidine blue staining that can be performed within 25 s, provides excellent nuclear morphology, and is compatible with subsequent Papanicolaou staining of the slides. Furthermore, exposure to hazardous chemicals is minimized, as no organic solvents other than the alcohol-based fixative and glycerin for temporary mounting and coverslipping are required. We have used this protocol successfully in our ROSE practice and have not observed any discrepancies between toluidine blue- and permanent Papanicolaou-stained slides. SN - 1938-2650 UR - https://www.unboundmedicine.com/medline/citation/31962321/Ultrafast_Toluidine_Blue_Staining_for_Rapid_On-Site_Evaluation_of_Cytological_Smears L2 - https://www.karger.com?DOI=10.1159/000505254 DB - PRIME DP - Unbound Medicine ER -