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Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria.
Antibiotics (Basel). 2020 Jan 19; 9(1)A

Abstract

Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa (n = 25), Escherichia coli (n = 79), Klebsiella pneumoniae (n = 58)], Staphylococcus aureus (n = 34), Staphylococcus epidermidis (n = 14), and Enterococcus species (n = 15)) were used. The minimal inhibitory concentration (MIC) of CS was determined for all strains by using microdilution assay, and time-kill curve assays of representative reference and MDR strains of these bacteria were performed. Membrane permeation and bacterial reactive oxygen species (ROS) production were determined in presence of CS. CS MIC90 was 4-8 mg/L for all strains. CS was bactericidal, during 24 h, at 1× and 2× MIC against Gram-negative bacteria, and at 2× MIC against Gram-positive bacteria, and it did not affect their membrane permeabilization. Furthermore, we found that CS significantly increased the ROS production in Gram-negative with respect to Gram-positive bacteria at 24 h of incubation. Altogether, these results suggest that CS could be an effective treatment for infections caused by MDR Gram-negative and Gram-positive bacteria.

Authors+Show Affiliations

Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain.Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain.Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain.Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain.Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, University Hospital Virgen del Rocío, 41013 Seville, Spain. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, CSIC, University of Seville, 41013 Seville, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31963769

Citation

Vila Domínguez, Andrea, et al. "Antibacterial Activity of Colloidal Silver Against Gram-Negative and Gram-Positive Bacteria." Antibiotics (Basel, Switzerland), vol. 9, no. 1, 2020.
Vila Domínguez A, Ayerbe Algaba R, Miró Canturri A, et al. Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. Antibiotics (Basel). 2020;9(1).
Vila Domínguez, A., Ayerbe Algaba, R., Miró Canturri, A., Rodríguez Villodres, Á., & Smani, Y. (2020). Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. Antibiotics (Basel, Switzerland), 9(1). https://doi.org/10.3390/antibiotics9010036
Vila Domínguez A, et al. Antibacterial Activity of Colloidal Silver Against Gram-Negative and Gram-Positive Bacteria. Antibiotics (Basel). 2020 Jan 19;9(1) PubMed PMID: 31963769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial Activity of Colloidal Silver against Gram-Negative and Gram-Positive Bacteria. AU - Vila Domínguez,Andrea, AU - Ayerbe Algaba,Rafael, AU - Miró Canturri,Andrea, AU - Rodríguez Villodres,Ángel, AU - Smani,Younes, Y1 - 2020/01/19/ PY - 2019/12/05/received PY - 2020/01/10/revised PY - 2020/01/14/accepted PY - 2020/1/23/entrez PY - 2020/1/23/pubmed PY - 2020/1/23/medline KW - Gram-negative bacteria KW - Gram-positive bacteria KW - colloidal silver KW - multidrug-resistant bacteria JF - Antibiotics (Basel, Switzerland) JO - Antibiotics (Basel) VL - 9 IS - 1 N2 - Due to the emergence of antimicrobial resistance, new alternative therapies are needed. Silver was used to treat bacterial infections since antiquity due to its known antimicrobial properties. Here, we aimed to evaluate the in vitro activity of colloidal silver (CS) against multidrug-resistant (MDR) Gram-negative and Gram-positive bacteria. A total of 270 strains (Acinetobacter baumannii (n = 45), Pseudomonas aeruginosa (n = 25), Escherichia coli (n = 79), Klebsiella pneumoniae (n = 58)], Staphylococcus aureus (n = 34), Staphylococcus epidermidis (n = 14), and Enterococcus species (n = 15)) were used. The minimal inhibitory concentration (MIC) of CS was determined for all strains by using microdilution assay, and time-kill curve assays of representative reference and MDR strains of these bacteria were performed. Membrane permeation and bacterial reactive oxygen species (ROS) production were determined in presence of CS. CS MIC90 was 4-8 mg/L for all strains. CS was bactericidal, during 24 h, at 1× and 2× MIC against Gram-negative bacteria, and at 2× MIC against Gram-positive bacteria, and it did not affect their membrane permeabilization. Furthermore, we found that CS significantly increased the ROS production in Gram-negative with respect to Gram-positive bacteria at 24 h of incubation. Altogether, these results suggest that CS could be an effective treatment for infections caused by MDR Gram-negative and Gram-positive bacteria. SN - 2079-6382 UR - https://www.unboundmedicine.com/medline/citation/31963769/Antibacterial_Activity_of_Colloidal_Silver_against_Gram-Negative_and_Gram-Positive_Bacteria L2 - https://www.mdpi.com/resolver?pii=antibiotics9010036 DB - PRIME DP - Unbound Medicine ER -
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