The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.).
Theor Appl Genet. 2020 Apr; 133(4):1213-1225.TA

Abstract

KEY MESSAGE

The semidominant EMS-induced mutant w5 affects epicuticular wax deposition and mapped to an approximately 194-kb region on chromosome 7DL. Epicuticular wax is responsible for the glaucous appearance of plants and protects against many biotic and abiotic stresses. In wheat (Triticum aestivum L.), β-diketone is a major component of epicuticular wax in adult plants and contributes to the glaucousness of the aerial organs. In the present study, we identified an ethyl methanesulfonate-induced epicuticular wax-deficient mutant from the elite wheat cultivar Jimai22. Compared to wild-type Jimai22, the mutant lacked β-diketone and failed to form the glaucous coating on all aerial organs. The mutant also had significantly increased in cuticle permeability, based on water loss and chlorophyll efflux. Genetic analysis indicated that the mutant phenotype is controlled by a single, semidominant gene on the long arm of chromosome 7D, which was not allelic to the known wax gene loci W1-W4, and was therefore designated W5. W5 was finely mapped to an ~ 194-kb region (flanked by the molecular markers SSR2 and STARP11) that harbored four annotated genes according to the reference genome of Chinese Spring (RefSeq v1.0). Collectively, these data will broaden the knowledge of the genetic basis underlying epicuticular wax deposition in wheat.

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Authors+Show Affiliations

Li L

State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, The Ministry of Education/Key Laboratory of Crop Genetic Improvement, Beijing Municipality/China Agricultural University, Beijing, 100193, China. National Plant Gene Research Centre, Beijing, 100193, China.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024, China.

Zhang M

Dryland Agricultural Research Centre, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, China.

State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, The Ministry of Education/Key Laboratory of Crop Genetic Improvement, Beijing Municipality/China Agricultural University, Beijing, 100193, China. nizf@cau.edu.cn. National Plant Gene Research Centre, Beijing, 100193, China. nizf@cau.edu.cn.

MeSH

Chromosome MappingGenes, DominantGenes, PlantGenetic LociMutationPlant EpidermisPlant ProteinsTriticumWaxes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31965231

Citation

Li, Linghong, et al. "The Semidominant Mutation W5 Impairs Epicuticular Wax Deposition in Common Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 4, 2020, pp. 1213-1225.

Li L, Qi Z, Chai L, et al. The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.). Theor Appl Genet. 2020;133(4):1213-1225.

Li, L., Qi, Z., Chai, L., Chen, Z., Wang, T., Zhang, M., You, M., Peng, H., Yao, Y., Hu, Z., Xin, M., Guo, W., Sun, Q., & Ni, Z. (2020). The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(4), 1213-1225. https://doi.org/10.1007/s00122-020-03543-x

Li L, et al. The Semidominant Mutation W5 Impairs Epicuticular Wax Deposition in Common Wheat (Triticum Aestivum L.). Theor Appl Genet. 2020;133(4):1213-1225. PubMed PMID: 31965231.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.). AU - Li,Linghong, AU - Qi,Zhongqi, AU - Chai,Lingling, AU - Chen,Zhaoyan, AU - Wang,Tianya, AU - Zhang,Mingyi, AU - You,Mingshan, AU - Peng,Huiru, AU - Yao,Yingyin, AU - Hu,Zhaorong, AU - Xin,Mingming, AU - Guo,Weilong, AU - Sun,Qixin, AU - Ni,Zhongfu, Y1 - 2020/01/21/ PY - 2019/09/26/received PY - 2020/01/10/accepted PY - 2020/1/23/pubmed PY - 2020/11/11/medline PY - 2020/1/23/entrez SP - 1213 EP - 1225 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 4 N2 - KEY MESSAGE: The semidominant EMS-induced mutant w5 affects epicuticular wax deposition and mapped to an approximately 194-kb region on chromosome 7DL. Epicuticular wax is responsible for the glaucous appearance of plants and protects against many biotic and abiotic stresses. In wheat (Triticum aestivum L.), β-diketone is a major component of epicuticular wax in adult plants and contributes to the glaucousness of the aerial organs. In the present study, we identified an ethyl methanesulfonate-induced epicuticular wax-deficient mutant from the elite wheat cultivar Jimai22. Compared to wild-type Jimai22, the mutant lacked β-diketone and failed to form the glaucous coating on all aerial organs. The mutant also had significantly increased in cuticle permeability, based on water loss and chlorophyll efflux. Genetic analysis indicated that the mutant phenotype is controlled by a single, semidominant gene on the long arm of chromosome 7D, which was not allelic to the known wax gene loci W1-W4, and was therefore designated W5. W5 was finely mapped to an ~ 194-kb region (flanked by the molecular markers SSR2 and STARP11) that harbored four annotated genes according to the reference genome of Chinese Spring (RefSeq v1.0). Collectively, these data will broaden the knowledge of the genetic basis underlying epicuticular wax deposition in wheat. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31965231/The_semidominant_mutation_w5_impairs_epicuticular_wax_deposition_in_common_wheat__Triticum_aestivum_L___ DB - PRIME DP - Unbound Medicine ER -

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The semidominant mutation w5 impairs epicuticular wax deposition in common wheat (Triticum aestivum L.).Theor Appl Genet. 2020 Apr; 133(4):1213-1225.TA