Tags

Type your tag names separated by a space and hit enter

Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study.
Int J Reprod Biomed (Yazd) 2019; 17(12):907-914IJ

Abstract

Background

Testis is one of the most sensitive organs against the toxic effect of ionizing radiation. Exposure to even a low dose of radiation during radiotherapy, diagnostic radiology, or a radiological event could pose a threat to spermatogenesis. This may lead to temporary or permanent infertility or even transfer of genomic instability to the next generations.

Objective

In this study, we evaluated the protective effect of treatment with three natural antioxidants; resveratrol, alpha lipoic acid, and coenzyme Q10 on radiation-induced spermatogenesis injury.

Materials and Methods

30 NMRI mice (6-8 wk, 30 ± 5 gr) were randomly divided into six groups (n = 5/each) as 1) control; 2) radiation; 3) radiation + resveratrol; 4) radiation + alpha lipoic acid; 5) radiation + resveratrol + alpha lipoic acid; and 6) radiation+ Q10. Mice were treated with 100 mg/kg resveratrol or 200 mg/kg alpha lipoic acid or a combination of these drugs. Also, Q10 was administered at 200 mg/kg. All treatments were performed daily from two days before to 30 min before irradiation. Afterward, mice were exposed to 2 Gy 60 Co gamma rays; 37 days after irradiation, the testicular samples were collected and evaluated for histopathological parameters.

Results

Results showed that these agents are able to alleviate some toxicological parameters such as basal lamina and epididymis decreased sperm density. Also, all agents were able to increase Johnsen score. However, they could not protect against radiation-induced edema, atrophy of seminiferous tubules, and hyperplasia in Leydig cells.

Conclusion

This study indicates that resveratrol, alpha-lipoic acid, and Q10 have the potential to reduce some of the side effects of radiation on mice spermatogenesis. However, they cannot protect Leydig cells as a source of testosterone and seminiferous tubules as the location of sperm maturation.

Authors+Show Affiliations

Department of Radiology and Nuclear Medicine, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, Tehran, Iran.Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Physiology, College of Medicine, University of Misan, Misan, Iraq.Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31970312

Citation

Najafi, Masoud, et al. "Evaluating the Protective Effect of Resveratrol, Q10, and Alpha-lipoic Acid On Radiation-induced Mice Spermatogenesis Injury: a Histopathological Study." International Journal of Reproductive Biomedicine (Yazd, Iran), vol. 17, no. 12, 2019, pp. 907-914.
Najafi M, Cheki M, Amini P, et al. Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study. Int J Reprod Biomed (Yazd). 2019;17(12):907-914.
Najafi, M., Cheki, M., Amini, P., Javadi, A., Shabeeb, D., & Eleojo Musa, A. (2019). Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study. International Journal of Reproductive Biomedicine (Yazd, Iran), 17(12), pp. 907-914. doi:10.18502/ijrm.v17i12.5791.
Najafi M, et al. Evaluating the Protective Effect of Resveratrol, Q10, and Alpha-lipoic Acid On Radiation-induced Mice Spermatogenesis Injury: a Histopathological Study. Int J Reprod Biomed (Yazd). 2019;17(12):907-914. PubMed PMID: 31970312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluating the protective effect of resveratrol, Q10, and alpha-lipoic acid on radiation-induced mice spermatogenesis injury: A histopathological study. AU - Najafi,Masoud, AU - Cheki,Mohsen, AU - Amini,Peyman, AU - Javadi,Abdolreza, AU - Shabeeb,Dheyauldeen, AU - Eleojo Musa,Ahmed, Y1 - 2019/12/30/ PY - 2018/09/22/received PY - 2019/07/06/revised PY - 2019/07/20/accepted PY - 2020/1/24/entrez PY - 2020/1/24/pubmed PY - 2020/1/24/medline KW - Alpha-lipoic acid KW - Coenzyme Q10. KW - Resveratrol KW - Spermatogenesis KW - Radiation SP - 907 EP - 914 JF - International journal of reproductive biomedicine (Yazd, Iran) JO - Int J Reprod Biomed (Yazd) VL - 17 IS - 12 N2 - Background: Testis is one of the most sensitive organs against the toxic effect of ionizing radiation. Exposure to even a low dose of radiation during radiotherapy, diagnostic radiology, or a radiological event could pose a threat to spermatogenesis. This may lead to temporary or permanent infertility or even transfer of genomic instability to the next generations. Objective: In this study, we evaluated the protective effect of treatment with three natural antioxidants; resveratrol, alpha lipoic acid, and coenzyme Q10 on radiation-induced spermatogenesis injury. Materials and Methods: 30 NMRI mice (6-8 wk, 30 ± 5 gr) were randomly divided into six groups (n = 5/each) as 1) control; 2) radiation; 3) radiation + resveratrol; 4) radiation + alpha lipoic acid; 5) radiation + resveratrol + alpha lipoic acid; and 6) radiation+ Q10. Mice were treated with 100 mg/kg resveratrol or 200 mg/kg alpha lipoic acid or a combination of these drugs. Also, Q10 was administered at 200 mg/kg. All treatments were performed daily from two days before to 30 min before irradiation. Afterward, mice were exposed to 2 Gy 60 Co gamma rays; 37 days after irradiation, the testicular samples were collected and evaluated for histopathological parameters. Results: Results showed that these agents are able to alleviate some toxicological parameters such as basal lamina and epididymis decreased sperm density. Also, all agents were able to increase Johnsen score. However, they could not protect against radiation-induced edema, atrophy of seminiferous tubules, and hyperplasia in Leydig cells. Conclusion: This study indicates that resveratrol, alpha-lipoic acid, and Q10 have the potential to reduce some of the side effects of radiation on mice spermatogenesis. However, they cannot protect Leydig cells as a source of testosterone and seminiferous tubules as the location of sperm maturation. SN - 2476-4108 UR - https://www.unboundmedicine.com/medline/citation/31970312/Evaluating_the_protective_effect_of_resveratrol,_Q10,_and_alpha-lipoic_acid_on_radiation-induced_mice_spermatogenesis_injury:_A_histopathological_study L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31970312/ DB - PRIME DP - Unbound Medicine ER -