Tags

Type your tag names separated by a space and hit enter

Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice.
Microb Pathog. 2020 Apr; 141:103984.MP

Abstract

In this study, we assessed the pathogenicity of two H5N1 viruses isolated from crows in mice. Eighteen 6-8 weeks BALB/c mice each were intranasally inoculated with 106 EID50/ml of H5N1 viruses A/crow/India/03CA04/2015 (H9N2-PB2 reassortant H5N1) and A/crow/India/02CA01/2012 (Non-reassortant H5N1). The infected mice showed dullness, weight loss and ruffled fur coat. Histopathological examination of lungs showed severe congestion, haemorrhage, thrombus, fibrinous exudate in perivascular area, interstitial septal thickening, bronchiolitis and alveolitis leading to severe pneumonic changes and these lesions were less pronounced in reassortant virus infected mice. Viral replication was demonstrated in nasal mucosa, lungs, trachea and brain in both the groups. Brain, lung, nasal mucosa and trachea showed significantly higher viral RNA copies and presence of antigen in immunohistochemistry in both the groups. This study concludes that both the crow viruses caused morbidity and mortality in mice and the viruses were phenotypically highly virulent in mice. The H5N1 viruses isolated from synanthropes pose a serious public health concern and should be monitored continuously for their human spill-over.

Authors+Show Affiliations

ICAR- Indian Veterinary and Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India. Electronic address: Manoj.Kumar4@icar.gov.in.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.ICAR- Indian Veterinary and Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- Indian Veterinary and Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- Indian Veterinary and Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- Indian Veterinary and Research Institute, Izatnagar, Uttar Pradesh, India.ICAR- National Institute of High Security Animal Diseases, Bhopal, Madhya Pradesh, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31972269

Citation

Kombiah, Subbiah, et al. "Experimental Pathology of Two Highly Pathogenic H5N1 Viruses Isolated From Crows in BALB/c Mice." Microbial Pathogenesis, vol. 141, 2020, p. 103984.
Kombiah S, Kumar M, Murugkar HV, et al. Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice. Microb Pathog. 2020;141:103984.
Kombiah, S., Kumar, M., Murugkar, H. V., Nagarajan, S., Tosh, C., Senthil Kumar, D., Rajukumar, K., Gautam, S., Singh, R., Karikalan, M., Sharma, A. K., & Singh, V. P. (2020). Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice. Microbial Pathogenesis, 141, 103984. https://doi.org/10.1016/j.micpath.2020.103984
Kombiah S, et al. Experimental Pathology of Two Highly Pathogenic H5N1 Viruses Isolated From Crows in BALB/c Mice. Microb Pathog. 2020;141:103984. PubMed PMID: 31972269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice. AU - Kombiah,Subbiah, AU - Kumar,Manoj, AU - Murugkar,Harshad Vinayakrao, AU - Nagarajan,Shanmugasundaram, AU - Tosh,Chakradhar, AU - Senthil Kumar,Dhanapal, AU - Rajukumar,Katherukamem, AU - Gautam,Siddharth, AU - Singh,Rajendra, AU - Karikalan,Mathesh, AU - Sharma,Anil Kumar, AU - Singh,Vijendra Pal, Y1 - 2020/01/21/ PY - 2019/09/12/received PY - 2019/12/28/revised PY - 2020/01/15/accepted PY - 2020/1/24/pubmed PY - 2020/1/24/medline PY - 2020/1/24/entrez KW - Avian influenza KW - BALB/c mice KW - Crow isolates KW - H9N2-PB2 reassortant H5N1 SP - 103984 EP - 103984 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 141 N2 - In this study, we assessed the pathogenicity of two H5N1 viruses isolated from crows in mice. Eighteen 6-8 weeks BALB/c mice each were intranasally inoculated with 106 EID50/ml of H5N1 viruses A/crow/India/03CA04/2015 (H9N2-PB2 reassortant H5N1) and A/crow/India/02CA01/2012 (Non-reassortant H5N1). The infected mice showed dullness, weight loss and ruffled fur coat. Histopathological examination of lungs showed severe congestion, haemorrhage, thrombus, fibrinous exudate in perivascular area, interstitial septal thickening, bronchiolitis and alveolitis leading to severe pneumonic changes and these lesions were less pronounced in reassortant virus infected mice. Viral replication was demonstrated in nasal mucosa, lungs, trachea and brain in both the groups. Brain, lung, nasal mucosa and trachea showed significantly higher viral RNA copies and presence of antigen in immunohistochemistry in both the groups. This study concludes that both the crow viruses caused morbidity and mortality in mice and the viruses were phenotypically highly virulent in mice. The H5N1 viruses isolated from synanthropes pose a serious public health concern and should be monitored continuously for their human spill-over. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/31972269/Experimental_pathology_of_two_highly_pathogenic_H5N1_viruses_isolated_from_crows_in_BALB/c_mice L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(19)31623-7 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.