Tags

Type your tag names separated by a space and hit enter

Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells.
Mol Cell Endocrinol. 2020 Jan 20 [Online ahead of print]MC

Abstract

The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.

Authors+Show Affiliations

Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Post-Graduation Program in Medical Sciences: Endocrinology, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: bisouza@gmail.com.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil.Laboratory of Cell Differentiation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Post-Graduation Program in Medical Sciences: Endocrinology, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil.Laboratory for Tissue Bioengineering (BioTis), Inserm U1026, University of Bordeaux, Bordeaux, France.Laboratory for Stem Cells and Tissue Engineering, Post-Graduation Program in Cellular and Molecular Biology Applied to Health, Universidade Luterana do Brasil, Canoas, RS, Brazil.Post-Graduation Program in Health Sciences, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.Organ Procurement Organization, Santa Casa de Misericórdia de Porto Alegre. Porto Alegre, RS, Brazil.Transplant Center, Surgery Department, Hospital Dom Vicente Scherer, Santa Casa de Misericórdia de Porto Alegre. Porto Alegre, RS, Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Post-Graduation Program in Medical Sciences: Endocrinology, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.Laboratory for Stem Cells and Tissue Engineering, Post-Graduation Program in Cellular and Molecular Biology Applied to Health, Universidade Luterana do Brasil, Canoas, RS, Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Post-Graduation Program in Medical Sciences: Endocrinology, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Post-Graduation Program in Medical Sciences: Endocrinology, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31972330

Citation

de Souza, Bianca M., et al. "Improvement of Human Pancreatic Islet Quality After Co-culture With Human Adipose-derived Stem Cells." Molecular and Cellular Endocrinology, 2020, p. 110729.
de Souza BM, Rodrigues M, de Oliveira FS, et al. Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells. Mol Cell Endocrinol. 2020.
de Souza, B. M., Rodrigues, M., de Oliveira, F. S., da Silva, L. P. A., Bouças, A. P., Portinho, C. P., Dos Santos, B. P., Camassola, M., Rocha, D., Lysakowski, S., Martini, J., Leitão, C. B., Nardi, N. B., Bauer, A. C., & Crispim, D. (2020). Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells. Molecular and Cellular Endocrinology, 110729. https://doi.org/10.1016/j.mce.2020.110729
de Souza BM, et al. Improvement of Human Pancreatic Islet Quality After Co-culture With Human Adipose-derived Stem Cells. Mol Cell Endocrinol. 2020 Jan 20;110729. PubMed PMID: 31972330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells. AU - de Souza,Bianca M, AU - Rodrigues,Michelle, AU - de Oliveira,Fernanda S, AU - da Silva,Liana P A, AU - Bouças,Ana P, AU - Portinho,Ciro P, AU - Dos Santos,Bruno P, AU - Camassola,Melissa, AU - Rocha,Dagoberto, AU - Lysakowski,Simone, AU - Martini,Juliano, AU - Leitão,Cristiane B, AU - Nardi,Nance B, AU - Bauer,Andrea C, AU - Crispim,Daisy, Y1 - 2020/01/20/ PY - 2019/10/02/received PY - 2019/12/30/revised PY - 2020/01/17/accepted PY - 2020/1/24/entrez PY - 2020/1/24/pubmed PY - 2020/1/24/medline KW - Adipose-derived stem cells KW - Co-culture KW - Cytokines KW - Gene expression KW - Pancreatic islets SP - 110729 EP - 110729 JF - Molecular and cellular endocrinology JO - Mol. Cell. Endocrinol. N2 - The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality. SN - 1872-8057 UR - https://www.unboundmedicine.com/medline/citation/31972330/Improvement_of_human_pancreatic_islet_quality_after_co-culture_with_human_adipose-derived_stem_cells L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(20)30029-0 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.