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Antioxidant and antifungal activities of marrubiin, extracts and essential oil from Marrubium vulgare L. against pathogenic dermatophyte strains.
J Mycol Med. 2020 Apr; 30(1):100927.JM

Abstract

OBJECTIVE

Medicinal plants extracts and plant-derived compounds are one of the natural sources for discovering new antifungal agents, the objectives of this work were to investigate for the first time the antidermatophytic, antipathogenic activities of methanol, acetone extracts, and essential oil of Marrubium vulgare L. grown in Tunisia and its active compound marrubiin on pathogenic for animals and humans, such as some dermatophytes and pathogenic for plants, and to evaluate antioxidant activities of different extracts with consideration to their chemical compositions.

MATERIAL AND METHODS

Acetone and methanol extracts were evaluated by HPLC, the essential oil was also analyzed by GC/MS. PCL assay was used to determine the antioxidant activity.

RESULTS

Results showed that methanol and acetone extracts exhibited a significant antioxidant activity (261.41 and 272.90μmol TE/g respectively), while the lowest one was observed in the case of marrubiin and essential oil. The antifungal activity of different extracts, marrubiin and essential oil at two concentrations (20 and 100μg/mL) were screened against the dermatophytes fungi Microsporum gypseum, Microsporum canis, Arthroderma cajetani, Trichophyton mentagrophytes, Trichophyton tonsurans, Epidermophyton floccosum and against two fungi strains (Botrytis cinerea, Pythium ultimum). Among tested extracts, marrubiin at 100μg/mL showed about 50% inhibition for T. mentagrophytes and E. floccosum. The anti-phytopathogenic activity was also carried out, only marrubiin had in activity against B. cinerea at the highest dose (32.40%), while methanol extract of M.vulgare and marrubiin are able to increase the mycelial growth of P. ultimum at the highest concentration (45.15 and 40.30% respectively).

CONCLUSION

In our study, we conclude that M.vulgare and marrubiin can be used as natural antioxidants and antifungal agent for treatment of skin dermatophyte infections.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rezgui M
Faculty of Sciences of Tunisia, University Tunis El Manar, 2092 Tunis, Tunisia. Electronic address: mar_warezgui@hotmail.fr.
Majdoub N
Faculty of Sciences of Tunisia, University Tunis El Manar, 2092 Tunis, Tunisia.
Mabrouk B
Faculty of Sciences of Tunisia, University Tunis El Manar, 2092 Tunis, Tunisia.
Baldisserotto A
School of Pharmacy and Health Products, Department of Life Sciences and Biotechnology, University of Ferrara, Master Course in Cosmetic Sciences and Technology, Laboratory of Health Products, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy.
Bino A
School of Pharmacy and Health Products, Department of Life Sciences and Biotechnology, University of Ferrara, Master Course in Cosmetic Sciences and Technology, Laboratory of Health Products, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy.
Ben Kaab LB
Faculty of Sciences of Tunisia, University Tunis El Manar, 2092 Tunis, Tunisia.
Manfredini S
School of Pharmacy and Health Products, Department of Life Sciences and Biotechnology, University of Ferrara, Master Course in Cosmetic Sciences and Technology, Laboratory of Health Products, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy.

MeSH

AnimalsAntifungal AgentsAntioxidantsArthrodermataceaeDermatomycosesDiterpenesEpidermophytonHumansMarrubiumMicrobial Sensitivity TestsMicrosporumOils, VolatilePlant ExtractsPlant OilsTrichophyton

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31983544

Citation

Rezgui, M, et al. "Antioxidant and Antifungal Activities of Marrubiin, Extracts and Essential Oil From Marrubium Vulgare L. Against Pathogenic Dermatophyte Strains." Journal De Mycologie Medicale, vol. 30, no. 1, 2020, p. 100927.
Rezgui M, Majdoub N, Mabrouk B, et al. Antioxidant and antifungal activities of marrubiin, extracts and essential oil from Marrubium vulgare L. against pathogenic dermatophyte strains. J Mycol Med. 2020;30(1):100927.
Rezgui, M., Majdoub, N., Mabrouk, B., Baldisserotto, A., Bino, A., Ben Kaab, L. B., & Manfredini, S. (2020). Antioxidant and antifungal activities of marrubiin, extracts and essential oil from Marrubium vulgare L. against pathogenic dermatophyte strains. Journal De Mycologie Medicale, 30(1), 100927. https://doi.org/10.1016/j.mycmed.2020.100927
Rezgui M, et al. Antioxidant and Antifungal Activities of Marrubiin, Extracts and Essential Oil From Marrubium Vulgare L. Against Pathogenic Dermatophyte Strains. J Mycol Med. 2020;30(1):100927. PubMed PMID: 31983544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and antifungal activities of marrubiin, extracts and essential oil from Marrubium vulgare L. against pathogenic dermatophyte strains. AU - Rezgui,M, AU - Majdoub,N, AU - Mabrouk,B, AU - Baldisserotto,A, AU - Bino,A, AU - Ben Kaab,L B, AU - Manfredini,S, Y1 - 2020/01/11/ PY - 2017/05/04/received PY - 2019/03/22/revised PY - 2020/01/06/accepted PY - 2020/1/28/pubmed PY - 2020/9/9/medline PY - 2020/1/28/entrez KW - Antioxidant activity KW - Dermatophytes KW - Essential oil KW - M. vulgare KW - Marrubiin SP - 100927 EP - 100927 JF - Journal de mycologie medicale JO - J Mycol Med VL - 30 IS - 1 N2 - OBJECTIVE: Medicinal plants extracts and plant-derived compounds are one of the natural sources for discovering new antifungal agents, the objectives of this work were to investigate for the first time the antidermatophytic, antipathogenic activities of methanol, acetone extracts, and essential oil of Marrubium vulgare L. grown in Tunisia and its active compound marrubiin on pathogenic for animals and humans, such as some dermatophytes and pathogenic for plants, and to evaluate antioxidant activities of different extracts with consideration to their chemical compositions. MATERIAL AND METHODS: Acetone and methanol extracts were evaluated by HPLC, the essential oil was also analyzed by GC/MS. PCL assay was used to determine the antioxidant activity. RESULTS: Results showed that methanol and acetone extracts exhibited a significant antioxidant activity (261.41 and 272.90μmol TE/g respectively), while the lowest one was observed in the case of marrubiin and essential oil. The antifungal activity of different extracts, marrubiin and essential oil at two concentrations (20 and 100μg/mL) were screened against the dermatophytes fungi Microsporum gypseum, Microsporum canis, Arthroderma cajetani, Trichophyton mentagrophytes, Trichophyton tonsurans, Epidermophyton floccosum and against two fungi strains (Botrytis cinerea, Pythium ultimum). Among tested extracts, marrubiin at 100μg/mL showed about 50% inhibition for T. mentagrophytes and E. floccosum. The anti-phytopathogenic activity was also carried out, only marrubiin had in activity against B. cinerea at the highest dose (32.40%), while methanol extract of M.vulgare and marrubiin are able to increase the mycelial growth of P. ultimum at the highest concentration (45.15 and 40.30% respectively). CONCLUSION: In our study, we conclude that M.vulgare and marrubiin can be used as natural antioxidants and antifungal agent for treatment of skin dermatophyte infections. SN - 1773-0449 UR - https://www.unboundmedicine.com/medline/citation/31983544/Antioxidant_and_antifungal_activities_of_marrubiin_extracts_and_essential_oil_from_Marrubium_vulgare_L__against_pathogenic_dermatophyte_strains_ DB - PRIME DP - Unbound Medicine ER -