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Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice.
Plant Cell Rep. 2020 Apr; 39(4):457-472.PC

Abstract

KEY MESSAGE

Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-related proteins. High-throughput transcriptome investigations of plant immunity highlight the complexity of gene networks leading to incompatible interaction with the pathogen. Accumulating findings implicate papain-like cysteine proteases (PLCPs) as a central hub in plant defense. While diverse roles of PLCPs in different pathosystems have become more evident, information on gene networks and signaling pathways necessary to orchestrate downstream responses are lacking. To understand the biological significance of cysteine protease against Xanthomonas oryzae pv. oryzae, PLCP overexpression and knockout rice lines were generated. The pathogenicity test revealed the attenuation of Xanthomonas oryzae pv. oryzae race K3a virulence in transgenic lines which is ascribed to high hydrogen peroxide and free salicylic acid accumulation. Next-generation sequencing of RNA from transgenic and wild-type plants identified 1597 combined differentially expressed genes, 1269 of which were exclusively regulated in the transgenic libraries. It was found that PLCP aids rice to circumvent infection through the extensive activation of transduction signal and transcription factors that orchestrate downstream responses, including up-regulation of multiple pathogenesis-related proteins and biosynthesis of secondary metabolites.

Authors+Show Affiliations

Department of Crop Science, Chungbuk National University, Cheongju, 28644, Republic of Korea. Center for Studies in Biotechnology, Cebu Technological University Barili Campus, 6036, Barili, Cebu, Philippines.Department of Horticulture, Hankyong National University, Anseong, 17579, Republic of Korea. kykang@hknu.ac.kr.Department of Crop Science, Chungbuk National University, Cheongju, 28644, Republic of Korea. ygcho@cbnu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31993730

Citation

Niño, Marjohn C., et al. "Genome-wide Transcriptional Response of Papain-like Cysteine Protease-mediated Resistance Against Xanthomonas Oryzae Pv. Oryzae in Rice." Plant Cell Reports, vol. 39, no. 4, 2020, pp. 457-472.
Niño MC, Kang KK, Cho YG. Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice. Plant Cell Rep. 2020;39(4):457-472.
Niño, M. C., Kang, K. K., & Cho, Y. G. (2020). Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice. Plant Cell Reports, 39(4), 457-472. https://doi.org/10.1007/s00299-019-02502-1
Niño MC, Kang KK, Cho YG. Genome-wide Transcriptional Response of Papain-like Cysteine Protease-mediated Resistance Against Xanthomonas Oryzae Pv. Oryzae in Rice. Plant Cell Rep. 2020;39(4):457-472. PubMed PMID: 31993730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice. AU - Niño,Marjohn C, AU - Kang,Kwon Kyoo, AU - Cho,Yong-Gu, Y1 - 2020/01/28/ PY - 2019/10/24/received PY - 2019/12/17/accepted PY - 2020/1/30/pubmed PY - 2020/10/2/medline PY - 2020/1/30/entrez KW - Bacterial blight KW - Papain-like cysteine protease KW - RNA sequencing KW - Rice KW - Xanthomonas oryzae pv. oryzae SP - 457 EP - 472 JF - Plant cell reports JO - Plant Cell Rep VL - 39 IS - 4 N2 - KEY MESSAGE: Transgenic rice overexpressing PLCP attenuated the virulence of Xanthomonas oryzae pv. oryzae through extensive activation of transduction signal and transcription activities that orchestrate downstream responses including the biosynthesis of secondary metabolites and up-regulation of several pathogenesis-related proteins. High-throughput transcriptome investigations of plant immunity highlight the complexity of gene networks leading to incompatible interaction with the pathogen. Accumulating findings implicate papain-like cysteine proteases (PLCPs) as a central hub in plant defense. While diverse roles of PLCPs in different pathosystems have become more evident, information on gene networks and signaling pathways necessary to orchestrate downstream responses are lacking. To understand the biological significance of cysteine protease against Xanthomonas oryzae pv. oryzae, PLCP overexpression and knockout rice lines were generated. The pathogenicity test revealed the attenuation of Xanthomonas oryzae pv. oryzae race K3a virulence in transgenic lines which is ascribed to high hydrogen peroxide and free salicylic acid accumulation. Next-generation sequencing of RNA from transgenic and wild-type plants identified 1597 combined differentially expressed genes, 1269 of which were exclusively regulated in the transgenic libraries. It was found that PLCP aids rice to circumvent infection through the extensive activation of transduction signal and transcription factors that orchestrate downstream responses, including up-regulation of multiple pathogenesis-related proteins and biosynthesis of secondary metabolites. SN - 1432-203X UR - https://www.unboundmedicine.com/medline/citation/31993730/Genome_wide_transcriptional_response_of_papain_like_cysteine_protease_mediated_resistance_against_Xanthomonas_oryzae_pv__oryzae_in_rice_ L2 - http://dx.doi.org/10.1007/s00299-019-02502-1 DB - PRIME DP - Unbound Medicine ER -