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Involvement of TRPV4 ionotropic channel in tongue mechanical hypersensitivity in dry-tongue rats.
J Oral Sci. 2020; 62(1):13-17.JO

Abstract

Although xerostomia can cause persistent oral pain, the mechanisms underlying such pain are not well understood. To evaluate whether a phosphorylated p38 (pp38)-TRPV4 mechanism in trigeminal ganglion (TG) neurons has a role in mechanical hyperalgesia of dry tongue, a rat model of dry tongue was used to study the nocifensive reflex and pp38 and TRPV4 expression in TG neurons. The head-withdrawal reflex threshold for mechanical stimulation of the tongue was significantly lower in dry-tongue rats than in sham rats. The numbers of TRPV4- and pp38-immunoreactive cells in the TG were significantly higher in dry-tongue rats than in sham rats. Many TRPV4-IR cells were also pp38-immunoreactive. The number of TRPV1-IR cells was unchanged in the TG after induction of tongue dryness. Local injection of a TRPV4 blocker attenuated tongue mechanical hypersensitivity in dry-tongue rats. Intraganglionic injection of a selective p38 MAP kinase inhibitor eliminated tongue hypersensitivity in dry-tongue rats and suppressed TRPV4 expression in TG neurons. The present findings suggest that TRPV4 activation via p38 phosphorylation in TG neurons is involved in mechanical hypersensitivity associated with dry tongue. These mechanisms may have a role in pain associated with xerostomia.

Authors+Show Affiliations

Department of Physiology, Nihon University School of Dentistry.Department of Physiology, Nihon University School of Dentistry.Department of Physiology, Nihon University School of Dentistry.Department of Oral Diagnostic Sciences, Nihon University School of Dentistry.Department of Oral Diagnostic Sciences, Nihon University School of Dentistry.Department of Physiology, Nihon University School of Dentistry.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31996516

Citation

Chen, Jui Yen, et al. "Involvement of TRPV4 Ionotropic Channel in Tongue Mechanical Hypersensitivity in Dry-tongue Rats." Journal of Oral Science, vol. 62, no. 1, 2020, pp. 13-17.
Chen JY, Kubo A, Shinoda M, et al. Involvement of TRPV4 ionotropic channel in tongue mechanical hypersensitivity in dry-tongue rats. J Oral Sci. 2020;62(1):13-17.
Chen, J. Y., Kubo, A., Shinoda, M., Okada-Ogawa, A., Imamura, Y., & Iwata, K. (2020). Involvement of TRPV4 ionotropic channel in tongue mechanical hypersensitivity in dry-tongue rats. Journal of Oral Science, 62(1), 13-17. https://doi.org/10.2334/josnusd.18-0468
Chen JY, et al. Involvement of TRPV4 Ionotropic Channel in Tongue Mechanical Hypersensitivity in Dry-tongue Rats. J Oral Sci. 2020;62(1):13-17. PubMed PMID: 31996516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of TRPV4 ionotropic channel in tongue mechanical hypersensitivity in dry-tongue rats. AU - Chen,Jui Yen, AU - Kubo,Asako, AU - Shinoda,Masamichi, AU - Okada-Ogawa,Akiko, AU - Imamura,Yoshiki, AU - Iwata,Koichi, PY - 2020/1/31/entrez PY - 2020/1/31/pubmed PY - 2020/2/1/medline KW - dry tongue KW - mechanical allodynia KW - p38 KW - transient receptor potential vanilloid 4 KW - trigeminal ganglion SP - 13 EP - 17 JF - Journal of oral science JO - J Oral Sci VL - 62 IS - 1 N2 - Although xerostomia can cause persistent oral pain, the mechanisms underlying such pain are not well understood. To evaluate whether a phosphorylated p38 (pp38)-TRPV4 mechanism in trigeminal ganglion (TG) neurons has a role in mechanical hyperalgesia of dry tongue, a rat model of dry tongue was used to study the nocifensive reflex and pp38 and TRPV4 expression in TG neurons. The head-withdrawal reflex threshold for mechanical stimulation of the tongue was significantly lower in dry-tongue rats than in sham rats. The numbers of TRPV4- and pp38-immunoreactive cells in the TG were significantly higher in dry-tongue rats than in sham rats. Many TRPV4-IR cells were also pp38-immunoreactive. The number of TRPV1-IR cells was unchanged in the TG after induction of tongue dryness. Local injection of a TRPV4 blocker attenuated tongue mechanical hypersensitivity in dry-tongue rats. Intraganglionic injection of a selective p38 MAP kinase inhibitor eliminated tongue hypersensitivity in dry-tongue rats and suppressed TRPV4 expression in TG neurons. The present findings suggest that TRPV4 activation via p38 phosphorylation in TG neurons is involved in mechanical hypersensitivity associated with dry tongue. These mechanisms may have a role in pain associated with xerostomia. SN - 1880-4926 UR - https://www.unboundmedicine.com/medline/citation/31996516/Involvement_of_TRPV4_ionotropic_channel_in_tongue_mechanical_hypersensitivity_in_dry_tongue_rats_ L2 - https://dx.doi.org/10.2334/josnusd.18-0468 DB - PRIME DP - Unbound Medicine ER -