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Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy.
Plant Sci. 2020 Mar; 292:110370.PS

Abstract

This study investigated the dynamic of zinc (Zn) uptake and the root-to-shoot Zn-transport when supplied as ZnSO4 (aq) or Zn-EDTA (aq) in soybean seedlings using in vivo X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XANES). The time-resolved X-ray fluorescence showed that plants absorbed ca. 10-fold more Zn from ZnSO4 (aq) than from Zn-EDTA (aq). However, the uptake velocity did not influence the amount of Zn in the stem. It let furthermore appear that the plants were able to reduce the absorption of Zn from Zn-EDTA (aq) earlier than ZnSO4 (aq). Thus, the entrance of Zn2+ into the roots is not necessarily accompanied by SO42-(aq). Regardless the source, the Zn distribution and its transport in the stem were spatially correlated to the bundles and cortex nearby the epidermal cells. Its chemical speciation showed that Zn is neither transported as ZnSO4(aq) nor as Zn-EDTA(aq), indicating that these compounds are retained in the roots or biotransformed on in the root-solution interface. Zn2+ was long-distance transported complexed by organic molecules such as histidine, malate, and citrate, and the proportion of ligands was affected by the concentration of Zn2+ in the stem rather than by the type of Zn source.

Authors+Show Affiliations

University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: gabriel.montanha@usp.br.University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: eduardosr07@gmail.com.University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: sara.zachi97@gmail.com.University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: edualm@cena.usp.br.São Paulo State University (UNESP), Rua Domingos da Costa Lopes 780, 17602-496, Tupã, São Paulo, Brazil. Electronic address: andre.reis@unesp.br.University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: jlavres@cena.usp.br.University of São Paulo (USP), Center of Nuclear Energy in Agriculture (CENA), Avenida Centenário 303, 13416-000, Piracicaba, São Paulo, Brazil. Electronic address: hudson@cena.usp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32005376

Citation

Montanha, Gabriel S., et al. "Zinc Uptake From ZnSO4 (aq) and Zn-EDTA (aq) and Its Root-to-shoot Transport in Soybean Plants (Glycine Max) Probed By Time-resolved in Vivo X-ray Spectroscopy." Plant Science : an International Journal of Experimental Plant Biology, vol. 292, 2020, p. 110370.
Montanha GS, Rodrigues ES, Romeu SLZ, et al. Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy. Plant Sci. 2020;292:110370.
Montanha, G. S., Rodrigues, E. S., Romeu, S. L. Z., de Almeida, E., Reis, A. R., Lavres, J., & Pereira de Carvalho, H. W. (2020). Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy. Plant Science : an International Journal of Experimental Plant Biology, 292, 110370. https://doi.org/10.1016/j.plantsci.2019.110370
Montanha GS, et al. Zinc Uptake From ZnSO4 (aq) and Zn-EDTA (aq) and Its Root-to-shoot Transport in Soybean Plants (Glycine Max) Probed By Time-resolved in Vivo X-ray Spectroscopy. Plant Sci. 2020;292:110370. PubMed PMID: 32005376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zinc uptake from ZnSO4 (aq) and Zn-EDTA (aq) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy. AU - Montanha,Gabriel S, AU - Rodrigues,Eduardo S, AU - Romeu,Sara L Z, AU - de Almeida,Eduardo, AU - Reis,André R, AU - Lavres,José,Jr AU - Pereira de Carvalho,Hudson W, Y1 - 2019/12/12/ PY - 2019/07/23/received PY - 2019/12/02/revised PY - 2019/12/04/accepted PY - 2020/2/2/entrez PY - 2020/2/2/pubmed PY - 2020/9/1/medline KW - Chemical speciation KW - Micronutrient transportationin vivo kinetics KW - XANES KW - XRF KW - Zinc uptake SP - 110370 EP - 110370 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 292 N2 - This study investigated the dynamic of zinc (Zn) uptake and the root-to-shoot Zn-transport when supplied as ZnSO4 (aq) or Zn-EDTA (aq) in soybean seedlings using in vivo X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XANES). The time-resolved X-ray fluorescence showed that plants absorbed ca. 10-fold more Zn from ZnSO4 (aq) than from Zn-EDTA (aq). However, the uptake velocity did not influence the amount of Zn in the stem. It let furthermore appear that the plants were able to reduce the absorption of Zn from Zn-EDTA (aq) earlier than ZnSO4 (aq). Thus, the entrance of Zn2+ into the roots is not necessarily accompanied by SO42-(aq). Regardless the source, the Zn distribution and its transport in the stem were spatially correlated to the bundles and cortex nearby the epidermal cells. Its chemical speciation showed that Zn is neither transported as ZnSO4(aq) nor as Zn-EDTA(aq), indicating that these compounds are retained in the roots or biotransformed on in the root-solution interface. Zn2+ was long-distance transported complexed by organic molecules such as histidine, malate, and citrate, and the proportion of ligands was affected by the concentration of Zn2+ in the stem rather than by the type of Zn source. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/32005376/Zinc_uptake_from_ZnSO4__aq__and_Zn_EDTA__aq__and_its_root_to_shoot_transport_in_soybean_plants__Glycine_max__probed_by_time_resolved_in_vivo_X_ray_spectroscopy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-9452(19)31543-2 DB - PRIME DP - Unbound Medicine ER -