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Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis.
Front Genet. 2019; 10:1321.FG

Abstract

The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the identification and development new drugs based on the anticoagulant effects of H. manillensis bites; however, data regarding its genome remain unclear. This study aimed to determine the genome sequence of an adult Asian Buffalo leech. We generated a draft assembly of 151.8 Mb and a N50 scaffold of 2.28 Mb. Predictions indicated that the assembled genome contained 21,005 protein-coding genes. Up to 17,865 genes were annotated in multiple databases including Gene Ontology. Sixteen anticoagulant proteins with a Hirudin or Antistasin domain were identified. This study is the first to report the whole-genome sequence of the Asian Buffalo leech, an important sanguivorous leech of clinical significance. The quality of the assembly is comparable to those of other annelids. These data will help further the current understanding of the biological mechanisms and genetic characteristics of leeches and serve as a valuable resource for future studies.

Authors+Show Affiliations

College of Life Sciences, Shaanxi Normal University, Xi'an, China.Center for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi'an, China.College of Biomedical Sciences & Department of Biological Sciences, Xuzhou Medical University, Xuzhou, China.Nextomics Biosciences Institute, Wuhan, China.Nextomics Biosciences Institute, Wuhan, China.College of Life Sciences, Shaanxi Normal University, Xi'an, China.College of Life Sciences, Shaanxi Normal University, Xi'an, China.College of Life Sciences, Shaanxi Normal University, Xi'an, China.Center for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi'an, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32010187

Citation

Guan, De-Long, et al. "Draft Genome of the Asian Buffalo Leech Hirudinaria Manillensis." Frontiers in Genetics, vol. 10, 2019, p. 1321.
Guan DL, Yang J, Liu YK, et al. Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis. Front Genet. 2019;10:1321.
Guan, D. L., Yang, J., Liu, Y. K., Li, Y., Mi, D., Ma, L. B., Wang, Z. Z., Xu, S. Q., & Qiu, Q. (2019). Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis. Frontiers in Genetics, 10, 1321. https://doi.org/10.3389/fgene.2019.01321
Guan DL, et al. Draft Genome of the Asian Buffalo Leech Hirudinaria Manillensis. Front Genet. 2019;10:1321. PubMed PMID: 32010187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Draft Genome of the Asian Buffalo Leech Hirudinaria manillensis. AU - Guan,De-Long, AU - Yang,Jie, AU - Liu,Ying-Kui, AU - Li,Yuan, AU - Mi,Da, AU - Ma,Li-Bin, AU - Wang,Zhe-Zhi, AU - Xu,Sheng-Quan, AU - Qiu,Qiang, Y1 - 2020/01/16/ PY - 2019/06/18/received PY - 2019/12/04/accepted PY - 2020/2/4/entrez PY - 2020/2/6/pubmed PY - 2020/2/6/medline KW - Asian buffalo leech KW - Hirudin/Antistasin gene family KW - Hirudinaria manillensis KW - genome assembly and annotation KW - sequencing SP - 1321 EP - 1321 JF - Frontiers in genetics JO - Front Genet VL - 10 N2 - The Asian Buffalo leech, Hirudinaria manillensis, is an aquatic sanguivorous species distributed widely in Southeast Asia. H. manillensis has long been used clinically for bloodletting and other medical purposes. Recent studies have focused on artificial culturing, strain optimization, and the identification and development new drugs based on the anticoagulant effects of H. manillensis bites; however, data regarding its genome remain unclear. This study aimed to determine the genome sequence of an adult Asian Buffalo leech. We generated a draft assembly of 151.8 Mb and a N50 scaffold of 2.28 Mb. Predictions indicated that the assembled genome contained 21,005 protein-coding genes. Up to 17,865 genes were annotated in multiple databases including Gene Ontology. Sixteen anticoagulant proteins with a Hirudin or Antistasin domain were identified. This study is the first to report the whole-genome sequence of the Asian Buffalo leech, an important sanguivorous leech of clinical significance. The quality of the assembly is comparable to those of other annelids. These data will help further the current understanding of the biological mechanisms and genetic characteristics of leeches and serve as a valuable resource for future studies. SN - 1664-8021 UR - https://www.unboundmedicine.com/medline/citation/32010187/Draft_Genome_of_the_Asian_Buffalo_Leech_Hirudinaria_manillensis L2 - https://doi.org/10.3389/fgene.2019.01321 DB - PRIME DP - Unbound Medicine ER -
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