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Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus.
Sci Total Environ. 2020 May 01; 715:136797.ST

Abstract

We previously demonstrated that N-acetylcysteine (NAC) could reduce the toxicity of silver (Ag) materials (nanoparticles (NPs) and Ag nitrate) to the soil invertebrate Enchytraeus crypticus (Oligochaeta). It remains however, unclear whether the antitoxic mechanism of NAC was caused by NAC-Ag binding in the soil or inside the organisms. This study aimed at determining the bioavailability of Ag in the soil in a 21-day toxicity test as well as the Ag uptake and elimination kinetics in E. crypticus exposed to AgNPs in LUFA 2.2 standard soil amended with low (100 mg/kg dry soil) and high (600 mg/kg dry soil) NAC concentrations. The addition of NAC to the soil alleviated the toxicity of AgNPs by decreasing the internal Ag concentration of E. crypticus in a dose-dependent manner. Indeed, NAC reduced the binding of Ag to the soil, which probably was due to the formation of soluble but biologically unavailable Ag-cysteine complexes. The reduced Ag uptake in the enchytraeids was explained from an increased elimination at high NAC levels. These findings reinforce the view that metal complexing-compounds like NAC play a key role in the modulation of AgNP toxicity and bioavailability in terrestrial environments. Further, it may inform on the potential of NAC as a remediation solution for Ag or other metal-contaminated soils.

Authors+Show Affiliations

Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, São Paulo 13083-970, Brazil; Department of Ecological Science, Faculty of Science, Vrije Universiteit, Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands. Electronic address: m115623@dac.unicamp.br.Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, São Paulo 13083-970, Brazil.Department of Ecological Science, Faculty of Science, Vrije Universiteit, Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32014764

Citation

Mendonça, Monique Culturato Padilha, et al. "Protective Effect of N-acetylcysteine On the Toxicity of Silver Nanoparticles: Bioavailability and Toxicokinetics in Enchytraeus Crypticus." The Science of the Total Environment, vol. 715, 2020, p. 136797.
Mendonça MCP, de Jesus MB, van Gestel CAM. Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus. Sci Total Environ. 2020;715:136797.
Mendonça, M. C. P., de Jesus, M. B., & van Gestel, C. A. M. (2020). Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus. The Science of the Total Environment, 715, 136797. https://doi.org/10.1016/j.scitotenv.2020.136797
Mendonça MCP, de Jesus MB, van Gestel CAM. Protective Effect of N-acetylcysteine On the Toxicity of Silver Nanoparticles: Bioavailability and Toxicokinetics in Enchytraeus Crypticus. Sci Total Environ. 2020 May 1;715:136797. PubMed PMID: 32014764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus. AU - Mendonça,Monique Culturato Padilha, AU - de Jesus,Marcelo Bispo, AU - van Gestel,Cornelis A M, Y1 - 2020/01/18/ PY - 2019/10/14/received PY - 2020/01/14/revised PY - 2020/01/17/accepted PY - 2020/2/6/pubmed PY - 2020/4/2/medline PY - 2020/2/5/entrez KW - Bioavailability KW - Metallic nanoparticles KW - Nanoecotoxicology KW - Thiol compounds SP - 136797 EP - 136797 JF - The Science of the total environment JO - Sci Total Environ VL - 715 N2 - We previously demonstrated that N-acetylcysteine (NAC) could reduce the toxicity of silver (Ag) materials (nanoparticles (NPs) and Ag nitrate) to the soil invertebrate Enchytraeus crypticus (Oligochaeta). It remains however, unclear whether the antitoxic mechanism of NAC was caused by NAC-Ag binding in the soil or inside the organisms. This study aimed at determining the bioavailability of Ag in the soil in a 21-day toxicity test as well as the Ag uptake and elimination kinetics in E. crypticus exposed to AgNPs in LUFA 2.2 standard soil amended with low (100 mg/kg dry soil) and high (600 mg/kg dry soil) NAC concentrations. The addition of NAC to the soil alleviated the toxicity of AgNPs by decreasing the internal Ag concentration of E. crypticus in a dose-dependent manner. Indeed, NAC reduced the binding of Ag to the soil, which probably was due to the formation of soluble but biologically unavailable Ag-cysteine complexes. The reduced Ag uptake in the enchytraeids was explained from an increased elimination at high NAC levels. These findings reinforce the view that metal complexing-compounds like NAC play a key role in the modulation of AgNP toxicity and bioavailability in terrestrial environments. Further, it may inform on the potential of NAC as a remediation solution for Ag or other metal-contaminated soils. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/32014764/Protective_effect_of_N_acetylcysteine_on_the_toxicity_of_silver_nanoparticles:_Bioavailability_and_toxicokinetics_in_Enchytraeus_crypticus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)30307-7 DB - PRIME DP - Unbound Medicine ER -