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Bisphenol A induces a shift in sex differentiation gene expression with testis-ova or sex reversal in Japanese medaka (Oryzias latipes).
J Appl Toxicol. 2020 06; 40(6):804-814.JA

Abstract

Bisphenol A (BPA), a very important raw material in the plastics industry, is an endocrine-disrupting chemical in teleost fish. Although BPA induces testis-ova and sex reversal in teleost fish species, the molecular mechanism remains unclear. We evaluated the effects of BPA (measured concentrations: 45, 92, 326, 1030 and 3406 μg/L) on Japanese medaka (Oryzias latipes) using OECD TG234 (2011, Fish Sexual Development Test, OECD Guidelines for the Testing of Chemicals, Section 2). BPA at 1030 and 3406 μg/L induced testis-ova and sex reversal with female-type secondary sexual characteristics in XY males at 30 and 60 days posthatching (dph). Then we examined the BPA effect on the expression of sex differentiation genes related to the testis-ova and sex reversal in XY medaka. BPA exposure (1030 and 3406 μg/L) suppressed gsdf mRNA expression and increased cyp19a1a mRNA expression in XY individuals at stage 38 and 30 dph, although foxl2 mRNA expression showed no change. Interestingly, the concentration of BPA that suppressed gsdf mRNA expression at the larval stage was consistent with that needed to induce testis-ova and sex reversal. These results suggest that the gsdf gene at the embryonic stage can be used as a useful biomarker for predicting the impact of estrogenic endocrine-disrupting chemicals on sexual differentiation in Japanese medaka.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Horie Y
Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Kanazawa N
Faculty of Systems Science and Technology, Akita Prefectural University, Akita, Japan.
Takahashi C
Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Tatarazako N
Department of Science and Technology for Biological Resources and Environment, Graduate School of Agriculture, Matsuyama, Japan.
Iguchi T
Nanobioscience, Yokohama City University, Yokohama, Japan.

MeSH

AnimalsAromataseBenzhydryl CompoundsEndocrine DisruptorsFemaleFish ProteinsForkhead Box Protein L2Gene Expression Regulation, DevelopmentalMaleOryziasOvumPhenolsSex DifferentiationTestisTransforming Growth Factor betaWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32020657

Citation

Horie, Yoshifumi, et al. "Bisphenol a Induces a Shift in Sex Differentiation Gene Expression With Testis-ova or Sex Reversal in Japanese Medaka (Oryzias Latipes)." Journal of Applied Toxicology : JAT, vol. 40, no. 6, 2020, pp. 804-814.
Horie Y, Kanazawa N, Takahashi C, et al. Bisphenol A induces a shift in sex differentiation gene expression with testis-ova or sex reversal in Japanese medaka (Oryzias latipes). J Appl Toxicol. 2020;40(6):804-814.
Horie, Y., Kanazawa, N., Takahashi, C., Tatarazako, N., & Iguchi, T. (2020). Bisphenol A induces a shift in sex differentiation gene expression with testis-ova or sex reversal in Japanese medaka (Oryzias latipes). Journal of Applied Toxicology : JAT, 40(6), 804-814. https://doi.org/10.1002/jat.3945
Horie Y, et al. Bisphenol a Induces a Shift in Sex Differentiation Gene Expression With Testis-ova or Sex Reversal in Japanese Medaka (Oryzias Latipes). J Appl Toxicol. 2020;40(6):804-814. PubMed PMID: 32020657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bisphenol A induces a shift in sex differentiation gene expression with testis-ova or sex reversal in Japanese medaka (Oryzias latipes). AU - Horie,Yoshifumi, AU - Kanazawa,Nobuhiko, AU - Takahashi,Chiho, AU - Tatarazako,Norihisa, AU - Iguchi,Taisen, Y1 - 2020/02/04/ PY - 2019/11/07/received PY - 2019/12/10/revised PY - 2019/12/24/accepted PY - 2020/2/6/pubmed PY - 2021/7/13/medline PY - 2020/2/6/entrez KW - Oryzias latipes KW - cyp19a1a KW - ecotoxicity KW - foxl2 KW - gsdf KW - sex reversal SP - 804 EP - 814 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 40 IS - 6 N2 - Bisphenol A (BPA), a very important raw material in the plastics industry, is an endocrine-disrupting chemical in teleost fish. Although BPA induces testis-ova and sex reversal in teleost fish species, the molecular mechanism remains unclear. We evaluated the effects of BPA (measured concentrations: 45, 92, 326, 1030 and 3406 μg/L) on Japanese medaka (Oryzias latipes) using OECD TG234 (2011, Fish Sexual Development Test, OECD Guidelines for the Testing of Chemicals, Section 2). BPA at 1030 and 3406 μg/L induced testis-ova and sex reversal with female-type secondary sexual characteristics in XY males at 30 and 60 days posthatching (dph). Then we examined the BPA effect on the expression of sex differentiation genes related to the testis-ova and sex reversal in XY medaka. BPA exposure (1030 and 3406 μg/L) suppressed gsdf mRNA expression and increased cyp19a1a mRNA expression in XY individuals at stage 38 and 30 dph, although foxl2 mRNA expression showed no change. Interestingly, the concentration of BPA that suppressed gsdf mRNA expression at the larval stage was consistent with that needed to induce testis-ova and sex reversal. These results suggest that the gsdf gene at the embryonic stage can be used as a useful biomarker for predicting the impact of estrogenic endocrine-disrupting chemicals on sexual differentiation in Japanese medaka. SN - 1099-1263 UR - https://www.unboundmedicine.com/medline/citation/32020657/Bisphenol_A_induces_a_shift_in_sex_differentiation_gene_expression_with_testis_ova_or_sex_reversal_in_Japanese_medaka__Oryzias_latipes__ DB - PRIME DP - Unbound Medicine ER -