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Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats.
J Pediatr Gastroenterol Nutr. 2020 04; 70(4):430-435.JP

Abstract

OBJECTIVES

Intestinal failure-associated liver disease (IFALD) is a feared and life-threatening complication in neonates with intestinal failure (IF) receiving long-term total parenteral nutrition (TPN). This study aims to investigate the effect of exogenous secretin on liver pathology and hepatic function in a rat model of PN-associated liver disease (PNALD).

METHODS

Male Sprague-Dawley rats underwent right jugular venous catheterization to receive 14-day continuous TPN therapy. All rats were allocated into 3 groups: the Control group (n = 8) did not have surgery or TPN and was fed standard rat chow ad libitum; the TPN group (n = 8) underwent catheter insertion and TPN treatment; and the TPN/S group (n = 8) also underwent catheter insertion, TPN treatment, and exogenous secretin treatment (2.5 nmol · kg · day) daily. Fourteen days after initial surgery, we collected the animals' liver and blood samples for further test.

RESULTS

The TPN/S group had diminished direct bilirubin (TPN, 2.1 ± 0.7 μmol/L; TPN/S, 1.5 ± 0.2 μmol/L) and liver total bile acid levels (TPN, 144.5 ± 21.2 μmol/L; TPN/S, 123.4 ± 10.4 μmol/L) and improved histological outcomes compared with those in the TPN group. Exogenous secretin also enhanced the canalicular transporter (BSEP, 0.5-fold, P = 0.011) and inhibited the basolateral transporter (OSTA, -0.48-fold, P = 0.002; OSTB, -0.6-fold, P = 0.013) of liver bile acid.

CONCLUSIONS

In this animal model of PNALD, secretin may improve cholestasis by enhancing canalicular transport, inhibiting the basolateral export of liver bile acid, and eventually decreasing the total bile acid level in the liver. Exogenous secretin treatment may potentially prevent and treat IFALD in IF patients relying on long-term TPN therapy.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Cao X
Pediatric Surgery Department.
Feng F
Hepatobiliary Department, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Liu X
Pediatric Surgery Department.
Sun C
Pediatric Surgery Department.
Yang X
Pediatric Surgery Department.
Fang Y
Pediatric Surgery Department.
Li S
Pediatric Surgery Department.

MeSH

AnimalsHumansLiverLiver DiseasesMaleParenteral NutritionParenteral Nutrition, TotalRatsRats, Sprague-DawleySecretin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32022775

Citation

Cao, Xuehui, et al. "Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats." Journal of Pediatric Gastroenterology and Nutrition, vol. 70, no. 4, 2020, pp. 430-435.
Cao X, Feng F, Liu X, et al. Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats. J Pediatr Gastroenterol Nutr. 2020;70(4):430-435.
Cao, X., Feng, F., Liu, X., Sun, C., Yang, X., Fang, Y., & Li, S. (2020). Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats. Journal of Pediatric Gastroenterology and Nutrition, 70(4), 430-435. https://doi.org/10.1097/MPG.0000000000002622
Cao X, et al. Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats. J Pediatr Gastroenterol Nutr. 2020;70(4):430-435. PubMed PMID: 32022775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous Secretin Improves Parenteral Nutrition-associated Liver Disease in Rats. AU - Cao,Xuehui, AU - Feng,Feng, AU - Liu,Xuelai, AU - Sun,Chi, AU - Yang,Xiaofeng, AU - Fang,Yanbin, AU - Li,Suolin, PY - 2020/2/6/pubmed PY - 2021/6/22/medline PY - 2020/2/6/entrez SP - 430 EP - 435 JF - Journal of pediatric gastroenterology and nutrition JO - J Pediatr Gastroenterol Nutr VL - 70 IS - 4 N2 - OBJECTIVES: Intestinal failure-associated liver disease (IFALD) is a feared and life-threatening complication in neonates with intestinal failure (IF) receiving long-term total parenteral nutrition (TPN). This study aims to investigate the effect of exogenous secretin on liver pathology and hepatic function in a rat model of PN-associated liver disease (PNALD). METHODS: Male Sprague-Dawley rats underwent right jugular venous catheterization to receive 14-day continuous TPN therapy. All rats were allocated into 3 groups: the Control group (n = 8) did not have surgery or TPN and was fed standard rat chow ad libitum; the TPN group (n = 8) underwent catheter insertion and TPN treatment; and the TPN/S group (n = 8) also underwent catheter insertion, TPN treatment, and exogenous secretin treatment (2.5 nmol · kg · day) daily. Fourteen days after initial surgery, we collected the animals' liver and blood samples for further test. RESULTS: The TPN/S group had diminished direct bilirubin (TPN, 2.1 ± 0.7 μmol/L; TPN/S, 1.5 ± 0.2 μmol/L) and liver total bile acid levels (TPN, 144.5 ± 21.2 μmol/L; TPN/S, 123.4 ± 10.4 μmol/L) and improved histological outcomes compared with those in the TPN group. Exogenous secretin also enhanced the canalicular transporter (BSEP, 0.5-fold, P = 0.011) and inhibited the basolateral transporter (OSTA, -0.48-fold, P = 0.002; OSTB, -0.6-fold, P = 0.013) of liver bile acid. CONCLUSIONS: In this animal model of PNALD, secretin may improve cholestasis by enhancing canalicular transport, inhibiting the basolateral export of liver bile acid, and eventually decreasing the total bile acid level in the liver. Exogenous secretin treatment may potentially prevent and treat IFALD in IF patients relying on long-term TPN therapy. SN - 1536-4801 UR - https://www.unboundmedicine.com/medline/citation/32022775/Exogenous_Secretin_Improves_Parenteral_Nutrition_associated_Liver_Disease_in_Rats_ DB - PRIME DP - Unbound Medicine ER -