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Use of eosin for green spectroscopic determination of mebendazole.
Luminescence. 2020 Aug; 35(5):788-796.L

Abstract

New, sensitive, and reliable spectroscopic methods were constructed for the fast determination of the anthelmintic drug mebendazole. The methods depended on the reaction of the amino group in mebendazole with eosin in acidic medium forming an ion pair complex. The first method, Method I, relied on quenching of the native fluorescence of eosin after reaction with mebendazole at pH 3.7 using acetate buffer. Fluorescence quenching was measured at 538 nm after excitation at 518 nm. This method showed a linear response over the concentration range 5.0-20.0 μg/ml. The second method, Method II, was based on measuring the absorbance of the formed complex at 554 nm; the method showed good linearity from 7.0 to 22.0 μg/ml. Different parameters that influenced the formation of the reaction product were carefully investigated to reach the optimized conditions. A comparison between the proposed methods and a previous spectrophotometric method was carried out and there was no significant difference between them. The methods could be applied successfully to determine mebendazole in its tablet form. Moreover, the methods used water as diluting solvent, which made them compatible with the 'green' analytical chemistry principles. No organic solvents were used throughout the study.

Authors+Show Affiliations

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32026588

Citation

Roshdy, Aya, et al. "Use of Eosin for Green Spectroscopic Determination of Mebendazole." Luminescence : the Journal of Biological and Chemical Luminescence, vol. 35, no. 5, 2020, pp. 788-796.
Roshdy A, Elmansi H, Shalan S, et al. Use of eosin for green spectroscopic determination of mebendazole. Luminescence. 2020;35(5):788-796.
Roshdy, A., Elmansi, H., Shalan, S., & Elbrashy, A. (2020). Use of eosin for green spectroscopic determination of mebendazole. Luminescence : the Journal of Biological and Chemical Luminescence, 35(5), 788-796. https://doi.org/10.1002/bio.3785
Roshdy A, et al. Use of Eosin for Green Spectroscopic Determination of Mebendazole. Luminescence. 2020;35(5):788-796. PubMed PMID: 32026588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of eosin for green spectroscopic determination of mebendazole. AU - Roshdy,Aya, AU - Elmansi,Heba, AU - Shalan,Shereen, AU - Elbrashy,Amina, Y1 - 2020/02/05/ PY - 2019/10/22/received PY - 2019/12/09/revised PY - 2020/01/20/accepted PY - 2020/2/7/pubmed PY - 2020/2/7/medline PY - 2020/2/7/entrez KW - eosin KW - mebendazole KW - quenching KW - tablets SP - 788 EP - 796 JF - Luminescence : the journal of biological and chemical luminescence JO - Luminescence VL - 35 IS - 5 N2 - New, sensitive, and reliable spectroscopic methods were constructed for the fast determination of the anthelmintic drug mebendazole. The methods depended on the reaction of the amino group in mebendazole with eosin in acidic medium forming an ion pair complex. The first method, Method I, relied on quenching of the native fluorescence of eosin after reaction with mebendazole at pH 3.7 using acetate buffer. Fluorescence quenching was measured at 538 nm after excitation at 518 nm. This method showed a linear response over the concentration range 5.0-20.0 μg/ml. The second method, Method II, was based on measuring the absorbance of the formed complex at 554 nm; the method showed good linearity from 7.0 to 22.0 μg/ml. Different parameters that influenced the formation of the reaction product were carefully investigated to reach the optimized conditions. A comparison between the proposed methods and a previous spectrophotometric method was carried out and there was no significant difference between them. The methods could be applied successfully to determine mebendazole in its tablet form. Moreover, the methods used water as diluting solvent, which made them compatible with the 'green' analytical chemistry principles. No organic solvents were used throughout the study. SN - 1522-7243 UR - https://www.unboundmedicine.com/medline/citation/32026588/Use_of_eosin_for_green_spectroscopic_determination_of_mebendazole_ L2 - https://doi.org/10.1002/bio.3785 DB - PRIME DP - Unbound Medicine ER -
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