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Isolation of swine-derived Lactobacillus plantarum and its synergistic antimicrobial and health-promoting properties with ZnO nanoparticles.
J Appl Microbiol. 2020 Jun; 128(6):1764-1775.JA

Abstract

AIMS

The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO).

METHODS AND RESULTS

Of the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to β-lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food-borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19-fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg-1) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96- and 3·15-fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae.

CONCLUSIONS

A combination of nZnO and LFL exhibits potential health-benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects.

SIGNIFICANCE AND IMPACT OF THE STUDY

This study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on.

Authors+Show Affiliations

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.The 908th Hospital of Chinese People's Liberation Army Joint Logistic Support Force, Nanchang, People's Republic of China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32027448

Citation

Zheng, L, et al. "Isolation of Swine-derived Lactobacillus Plantarum and Its Synergistic Antimicrobial and Health-promoting Properties With ZnO Nanoparticles." Journal of Applied Microbiology, vol. 128, no. 6, 2020, pp. 1764-1775.
Zheng L, Hu Y, He X, et al. Isolation of swine-derived Lactobacillus plantarum and its synergistic antimicrobial and health-promoting properties with ZnO nanoparticles. J Appl Microbiol. 2020;128(6):1764-1775.
Zheng, L., Hu, Y., He, X., Zhao, Y., & Xu, H. (2020). Isolation of swine-derived Lactobacillus plantarum and its synergistic antimicrobial and health-promoting properties with ZnO nanoparticles. Journal of Applied Microbiology, 128(6), 1764-1775. https://doi.org/10.1111/jam.14605
Zheng L, et al. Isolation of Swine-derived Lactobacillus Plantarum and Its Synergistic Antimicrobial and Health-promoting Properties With ZnO Nanoparticles. J Appl Microbiol. 2020;128(6):1764-1775. PubMed PMID: 32027448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of swine-derived Lactobacillus plantarum and its synergistic antimicrobial and health-promoting properties with ZnO nanoparticles. AU - Zheng,L, AU - Hu,Y, AU - He,X, AU - Zhao,Y, AU - Xu,H, Y1 - 2020/02/20/ PY - 2019/10/18/received PY - 2020/01/06/revised PY - 2020/01/13/accepted PY - 2020/2/7/pubmed PY - 2020/8/22/medline PY - 2020/2/7/entrez KW - Lactobacillus plantarum KW - ZnO nanoparticles KW - antibacterial activity KW - diarrhoea KW - probiotic activity KW - weaned piglets SP - 1764 EP - 1775 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 128 IS - 6 N2 - AIMS: The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). METHODS AND RESULTS: Of the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to β-lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food-borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19-fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg-1) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96- and 3·15-fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae. CONCLUSIONS: A combination of nZnO and LFL exhibits potential health-benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects. SIGNIFICANCE AND IMPACT OF THE STUDY: This study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/32027448/Isolation_of_swine_derived_Lactobacillus_plantarum_and_its_synergistic_antimicrobial_and_health_promoting_properties_with_ZnO_nanoparticles_ L2 - https://doi.org/10.1111/jam.14605 DB - PRIME DP - Unbound Medicine ER -