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Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis.
Poult Sci. 2020 Feb; 99(2):1088-1095.PS

Abstract

Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. The aim of this study was to investigate the phenotypic and genotypic characteristics and antimicrobial resistance of recent APEC isolates. Of the 79 APEC isolates, the most predominant serogroup was O78 (16 isolates, 20.3%), followed by O2 (7 isolates, 8.9%) and O53 (7 isolates, 8.9%). Thirty-seven (46.8%) and six (7.6%) of the isolates belonged to phylogenetic groups D and B2, respectively, and presented as virulent extraintestinal E. coli. Among 5 analyzed virulence genes, the highest frequency was observed in hlyF (74 isolates, 93.7%), followed by iutA (72 isolates, 91.9%) gene. The distribution of the iss gene was significantly different between groups A/B1 and B2/D (P < 0.05). All group B2 isolates carried all 5 virulence genes. APEC isolates showed high resistance to ampicillin (83.5%), nalidixic acid (65.8%), tetracycline (64.6%), cephalothin (46.8%), and ciprofloxacin (46.8%). The β-lactamases-encoding genes blaTEM-1 (23 isolates, 29.1%), blaCTX-M-1 (4 isolates, 5.1%), and blaCTX-M-15 (3 isolates, 3.8%); the aminoglycoside-modifying enzyme gene aac(3)-II (4 isolates, 5.1%); and the plasmid-mediated quinolone genes qnrA (10 isolates, 12.7%) and qnrS (2 isolates, 2.5%) were identified in APEC isolates. The tetA (37 isolates, 46.8%) and sul2 (20 isolates, 25.3%) were the most prevalent among tetracycline and sulfonamide resistant isolates, respectively. This study indicates that APEC isolates harbor a variety of virulence and resistance genes; such genes are often associated with plasmids that facilitate their transmission between bacteria and should be continuously monitored to track APEC transmission in poultry farms.

Authors+Show Affiliations

College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.Samhwa GPS Breeding Agri. Inc., Hongseong 32291, Republic of Korea.Samhwa GPS Breeding Agri. Inc., Hongseong 32291, Republic of Korea.Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, 39762, USA.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: youngju@knu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32029145

Citation

Kim, Yeong Bin, et al. "Molecular Characterization of Avian Pathogenic Escherichia Coli From Broiler Chickens With Colibacillosis." Poultry Science, vol. 99, no. 2, 2020, pp. 1088-1095.
Kim YB, Yoon MY, Ha JS, et al. Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis. Poult Sci. 2020;99(2):1088-1095.
Kim, Y. B., Yoon, M. Y., Ha, J. S., Seo, K. W., Noh, E. B., Son, S. H., & Lee, Y. J. (2020). Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis. Poultry Science, 99(2), 1088-1095. https://doi.org/10.1016/j.psj.2019.10.047
Kim YB, et al. Molecular Characterization of Avian Pathogenic Escherichia Coli From Broiler Chickens With Colibacillosis. Poult Sci. 2020;99(2):1088-1095. PubMed PMID: 32029145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of avian pathogenic Escherichia coli from broiler chickens with colibacillosis. AU - Kim,Yeong Bin, AU - Yoon,Mi Young, AU - Ha,Jong Su, AU - Seo,Kwang Won, AU - Noh,Eun Bi, AU - Son,Se Hyun, AU - Lee,Young Ju, Y1 - 2019/11/22/ PY - 2019/08/03/received PY - 2019/10/19/revised PY - 2019/10/19/accepted PY - 2020/2/8/entrez PY - 2020/2/8/pubmed PY - 2020/6/2/medline KW - antimicrobial resistance KW - avian pathogenic Escherichia coli KW - broilers KW - phylogenetic group SP - 1088 EP - 1095 JF - Poultry science JO - Poult. Sci. VL - 99 IS - 2 N2 - Avian pathogenic Escherichia coli (APEC) causes extensive mortality in poultry flocks, leading to extensive economic losses. The aim of this study was to investigate the phenotypic and genotypic characteristics and antimicrobial resistance of recent APEC isolates. Of the 79 APEC isolates, the most predominant serogroup was O78 (16 isolates, 20.3%), followed by O2 (7 isolates, 8.9%) and O53 (7 isolates, 8.9%). Thirty-seven (46.8%) and six (7.6%) of the isolates belonged to phylogenetic groups D and B2, respectively, and presented as virulent extraintestinal E. coli. Among 5 analyzed virulence genes, the highest frequency was observed in hlyF (74 isolates, 93.7%), followed by iutA (72 isolates, 91.9%) gene. The distribution of the iss gene was significantly different between groups A/B1 and B2/D (P < 0.05). All group B2 isolates carried all 5 virulence genes. APEC isolates showed high resistance to ampicillin (83.5%), nalidixic acid (65.8%), tetracycline (64.6%), cephalothin (46.8%), and ciprofloxacin (46.8%). The β-lactamases-encoding genes blaTEM-1 (23 isolates, 29.1%), blaCTX-M-1 (4 isolates, 5.1%), and blaCTX-M-15 (3 isolates, 3.8%); the aminoglycoside-modifying enzyme gene aac(3)-II (4 isolates, 5.1%); and the plasmid-mediated quinolone genes qnrA (10 isolates, 12.7%) and qnrS (2 isolates, 2.5%) were identified in APEC isolates. The tetA (37 isolates, 46.8%) and sul2 (20 isolates, 25.3%) were the most prevalent among tetracycline and sulfonamide resistant isolates, respectively. This study indicates that APEC isolates harbor a variety of virulence and resistance genes; such genes are often associated with plasmids that facilitate their transmission between bacteria and should be continuously monitored to track APEC transmission in poultry farms. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/32029145/Molecular_characterization_of_avian_pathogenic_Escherichia_coli_from_broiler_chickens_with_colibacillosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0032-5791(19)49167-0 DB - PRIME DP - Unbound Medicine ER -