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Research Note: Effect of selection for body weight on the adipogenic conversion of turkey myogenic satellite cells by Syndecan-4 and its covalently attached N-glycosylation chains.
Poult Sci. 2020 Feb; 99(2):1209-1215.PS

Abstract

Adult myoblasts, satellite cells, will proliferate, and differentiate into myotubes in vitro. However, changes in environmental and nutritional conditions will result in the satellite cells differentiating into adipocyte-like cells synthesizing lipids. Prior research has shown that levels of N-glycosylation and heparan sulfate can promote or prevent the adipogenic conversion of myogenic satellite cells. Syndecan-4, an N-glycosylated heparan sulfate proteoglycan, has been shown to play key roles in satellite cell proliferation and migration. The objective of the current study was to determine if syndecan-4, and syndecan-4 N-glycosylation and heparan sulfate chain levels altered the conversion of satellite cells to an adipogenic cell fate and if growth selection affected the response of the satellite cells. Over-expression of syndecan-4, syndecan-4 without N-glycosylated chains but with its heparan sulfate chains attached, syndecan-4 without heparan sulfate chains with its N-glycosylation chains, and syndecan-4 without N-glycosylation and heparan sulfate chains was measured for lipid accumulation in pectoralis major muscle satellite cells isolated from the Randombred Control line 2 (RBC2) and 16 wk body weight (F line) turkeys. The F line was selected from the RBC2 line for only 16 wk body weight. Results from this study demonstrated that wild type levels of syndecan-4 and its covalently attached N-glycosylation chains play a key role in regulating the conversion of pectoralis major muscle satellite cells to an adipogenic lineage while selection for body weight was not a major contributing factor in this conversion.

Authors+Show Affiliations

The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster OH 44691, USA. Electronic address: Velleman.1@osu.edu.The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster OH 44691, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32029150

Citation

Velleman, Sandra G., and Cynthia S. Coy. "Research Note: Effect of Selection for Body Weight On the Adipogenic Conversion of Turkey Myogenic Satellite Cells By Syndecan-4 and Its Covalently Attached N-glycosylation Chains." Poultry Science, vol. 99, no. 2, 2020, pp. 1209-1215.
Velleman SG, Coy CS. Research Note: Effect of selection for body weight on the adipogenic conversion of turkey myogenic satellite cells by Syndecan-4 and its covalently attached N-glycosylation chains. Poult Sci. 2020;99(2):1209-1215.
Velleman, S. G., & Coy, C. S. (2020). Research Note: Effect of selection for body weight on the adipogenic conversion of turkey myogenic satellite cells by Syndecan-4 and its covalently attached N-glycosylation chains. Poultry Science, 99(2), 1209-1215. https://doi.org/10.1016/j.psj.2019.12.029
Velleman SG, Coy CS. Research Note: Effect of Selection for Body Weight On the Adipogenic Conversion of Turkey Myogenic Satellite Cells By Syndecan-4 and Its Covalently Attached N-glycosylation Chains. Poult Sci. 2020;99(2):1209-1215. PubMed PMID: 32029150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Research Note: Effect of selection for body weight on the adipogenic conversion of turkey myogenic satellite cells by Syndecan-4 and its covalently attached N-glycosylation chains. AU - Velleman,Sandra G, AU - Coy,Cynthia S, Y1 - 2020/01/24/ PY - 2019/06/26/received PY - 2019/10/01/accepted PY - 2020/2/8/entrez PY - 2020/2/8/pubmed PY - 2020/6/2/medline KW - N-glycosylation KW - adipogenesis KW - glycosaminoglycan KW - heparan sulfate KW - syndecan-4 SP - 1209 EP - 1215 JF - Poultry science JO - Poult Sci VL - 99 IS - 2 N2 - Adult myoblasts, satellite cells, will proliferate, and differentiate into myotubes in vitro. However, changes in environmental and nutritional conditions will result in the satellite cells differentiating into adipocyte-like cells synthesizing lipids. Prior research has shown that levels of N-glycosylation and heparan sulfate can promote or prevent the adipogenic conversion of myogenic satellite cells. Syndecan-4, an N-glycosylated heparan sulfate proteoglycan, has been shown to play key roles in satellite cell proliferation and migration. The objective of the current study was to determine if syndecan-4, and syndecan-4 N-glycosylation and heparan sulfate chain levels altered the conversion of satellite cells to an adipogenic cell fate and if growth selection affected the response of the satellite cells. Over-expression of syndecan-4, syndecan-4 without N-glycosylated chains but with its heparan sulfate chains attached, syndecan-4 without heparan sulfate chains with its N-glycosylation chains, and syndecan-4 without N-glycosylation and heparan sulfate chains was measured for lipid accumulation in pectoralis major muscle satellite cells isolated from the Randombred Control line 2 (RBC2) and 16 wk body weight (F line) turkeys. The F line was selected from the RBC2 line for only 16 wk body weight. Results from this study demonstrated that wild type levels of syndecan-4 and its covalently attached N-glycosylation chains play a key role in regulating the conversion of pectoralis major muscle satellite cells to an adipogenic lineage while selection for body weight was not a major contributing factor in this conversion. SN - 1525-3171 UR - https://www.unboundmedicine.com/medline/citation/32029150/Research_Note:_Effect_of_selection_for_body_weight_on_the_adipogenic_conversion_of_turkey_myogenic_satellite_cells_by_Syndecan_4_and_its_covalently_attached_N_glycosylation_chains_ DB - PRIME DP - Unbound Medicine ER -