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Synergistic improvement effect of nicotine-ghrelin co-injection into the anterior ventral tegmental area on morphine-induced amnesia.
Neuropeptides. 2020 Apr; 80:102025.N

Abstract

In the present study the effect of ghrelin or ghrelin/nicotine injection into the anterior ventral tegmental area (aVTA) on morphine-induced amnesia in passive avoidance learning have been evaluated. Also, the role of the aVTA nicotinic receptors in possible ghrelin-induced effects has been investigated. All animals were bilaterally implanted with chronic cannulas in the aVTA. A step-through type passive avoidance task was used for measurement of memory. We found that post-training subcutaneous (s.c.) injection of morphine (0.5-7.5 mg/kg) dose-dependently reduced the step-through latency, indicating morphine-induced amnesia. Post-training bilateral infusion of ghrelin (0.3, 1.5 and 3 nmol/μl) in a dose-dependent manner reversed amnesia induced by morphine (7.5 mg/kg, s.c.). Furthermore, reversal effect of ghrelin (3 nmol/μl) was blocked by pre-treatment of intra-aVTA administration of mecamylamine (1-3 μg/rat), a nicotinic acetylcholine receptor antagonist. Intra-aVTA administration of the higher dose of mecamylamine (3 μg/rat) into the aVTA by itself decreased the step-through latency and induced amnesia. In addition, post-training intra-aVTA administration of nicotine (0.25, 0.5, 1 μg/rat) which alone cannot affect memory consolidation, decreased significantly the amnesia induced by morphine (7.5 mg/kg, s.c.). Co-treatment of an ineffective dose of ghrelin (0.3 nmol/μl) with an ineffective dose of nicotine (0.25 μg/rat) significantly increased step-through latency of morphine (7.5 mg/kg, s.c.) treated animals, indicating the synergistic effect of the drugs. Taken together, our results suggest that intra-aVTA administration of ghrelin reversed morphine-induced amnesia and that ghrelin interacts synergistically with nicotine to mitigate morphine-induced amnesia.

Authors+Show Affiliations

Department of Biology, Faculty of Science, Arak University, Arak, Iran.Department of Biology, Payame Noor University (PNU), Tehran, Iran. Electronic address: nazari_farzaneh@yahoo.com.Department of Biology, Faculty of Science, Arak University, Arak, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32029269

Citation

Yadegary, Atena, et al. "Synergistic Improvement Effect of Nicotine-ghrelin Co-injection Into the Anterior Ventral Tegmental Area On Morphine-induced Amnesia." Neuropeptides, vol. 80, 2020, p. 102025.
Yadegary A, Nazari-Serenjeh F, Darbandi N. Synergistic improvement effect of nicotine-ghrelin co-injection into the anterior ventral tegmental area on morphine-induced amnesia. Neuropeptides. 2020;80:102025.
Yadegary, A., Nazari-Serenjeh, F., & Darbandi, N. (2020). Synergistic improvement effect of nicotine-ghrelin co-injection into the anterior ventral tegmental area on morphine-induced amnesia. Neuropeptides, 80, 102025. https://doi.org/10.1016/j.npep.2020.102025
Yadegary A, Nazari-Serenjeh F, Darbandi N. Synergistic Improvement Effect of Nicotine-ghrelin Co-injection Into the Anterior Ventral Tegmental Area On Morphine-induced Amnesia. Neuropeptides. 2020;80:102025. PubMed PMID: 32029269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic improvement effect of nicotine-ghrelin co-injection into the anterior ventral tegmental area on morphine-induced amnesia. AU - Yadegary,Atena, AU - Nazari-Serenjeh,Farzaneh, AU - Darbandi,Niloufar, Y1 - 2020/02/04/ PY - 2019/06/06/received PY - 2020/01/15/revised PY - 2020/01/29/accepted PY - 2020/2/8/pubmed PY - 2020/2/8/medline PY - 2020/2/8/entrez KW - Anterior ventral tegmental area KW - Avoidance learning KW - Ghrelin KW - Morphine KW - Nicotine SP - 102025 EP - 102025 JF - Neuropeptides JO - Neuropeptides VL - 80 N2 - In the present study the effect of ghrelin or ghrelin/nicotine injection into the anterior ventral tegmental area (aVTA) on morphine-induced amnesia in passive avoidance learning have been evaluated. Also, the role of the aVTA nicotinic receptors in possible ghrelin-induced effects has been investigated. All animals were bilaterally implanted with chronic cannulas in the aVTA. A step-through type passive avoidance task was used for measurement of memory. We found that post-training subcutaneous (s.c.) injection of morphine (0.5-7.5 mg/kg) dose-dependently reduced the step-through latency, indicating morphine-induced amnesia. Post-training bilateral infusion of ghrelin (0.3, 1.5 and 3 nmol/μl) in a dose-dependent manner reversed amnesia induced by morphine (7.5 mg/kg, s.c.). Furthermore, reversal effect of ghrelin (3 nmol/μl) was blocked by pre-treatment of intra-aVTA administration of mecamylamine (1-3 μg/rat), a nicotinic acetylcholine receptor antagonist. Intra-aVTA administration of the higher dose of mecamylamine (3 μg/rat) into the aVTA by itself decreased the step-through latency and induced amnesia. In addition, post-training intra-aVTA administration of nicotine (0.25, 0.5, 1 μg/rat) which alone cannot affect memory consolidation, decreased significantly the amnesia induced by morphine (7.5 mg/kg, s.c.). Co-treatment of an ineffective dose of ghrelin (0.3 nmol/μl) with an ineffective dose of nicotine (0.25 μg/rat) significantly increased step-through latency of morphine (7.5 mg/kg, s.c.) treated animals, indicating the synergistic effect of the drugs. Taken together, our results suggest that intra-aVTA administration of ghrelin reversed morphine-induced amnesia and that ghrelin interacts synergistically with nicotine to mitigate morphine-induced amnesia. SN - 1532-2785 UR - https://www.unboundmedicine.com/medline/citation/32029269/Synergistic_improvement_effect_of_nicotine_ghrelin_co_injection_into_the_anterior_ventral_tegmental_area_on_morphine_induced_amnesia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4179(19)30089-7 DB - PRIME DP - Unbound Medicine ER -