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TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae.
Sci Rep. 2020 02 06; 10(1):2036.SR

Abstract

The xa5 gene encodes a basal transcription factor (TFIIAγ) protein with wide spectrum resistance to bacterial blight caused by Xanthomonas oryzae pv. Oryzae (Xoo) in rice. It was only found in a few rice ecotypes, and the recessive characteristics limited its application in breeding. Here, we employed a TALEN-based technique to edit its dominant allelic TFIIAγ5 and obtained many mutant TFIIAγ5 genes. Most of them reduced rice susceptibility to varying degrees when the plants were challenged with the Xoo. In particular, the knocked-out TFIIAγ5 can reduce the rice susceptibility significantly, although it cannot reach the xa5-mediated resistance level, indicating TFIIAγ5 is a major component involved in disease susceptibility. In addition, the mutant encoding the protein with deletion of the 32nd amino acid or amino acid insertion between 32nd and 33rd site confers rice with the similar resistance to that of the knocked-out TFIIAγ5. Thus, the amino acids around 32nd site are also the important action sites of TFIIAγ5 besides the 39th amino acid previously reported. Moreover, the integration of xa5 into TFIIAγ5-knockout plants conferred them with a similar resistance as IRBB5, the rice variety containing the homozygous xa5 gene. Thus, TFIIAγ5 was not simply regarded as a resistant or a susceptible locus, as the substitution of amino acids might shift its functions.

Authors+Show Affiliations

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. ghjiang@genetics.ac.cn.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. wxzhai@genetics.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32029874

Citation

Han, Jin, et al. "TALEN-based Editing of TFIIAy5 Changes Rice Response to Xanthomonas Oryzae Pv. Oryzae." Scientific Reports, vol. 10, no. 1, 2020, p. 2036.
Han J, Xia Z, Liu P, et al. TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae. Sci Rep. 2020;10(1):2036.
Han, J., Xia, Z., Liu, P., Li, C., Wang, Y., Guo, L., Jiang, G., & Zhai, W. (2020). TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae. Scientific Reports, 10(1), 2036. https://doi.org/10.1038/s41598-020-59052-w
Han J, et al. TALEN-based Editing of TFIIAy5 Changes Rice Response to Xanthomonas Oryzae Pv. Oryzae. Sci Rep. 2020 02 6;10(1):2036. PubMed PMID: 32029874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TALEN-based editing of TFIIAy5 changes rice response to Xanthomonas oryzae pv. Oryzae. AU - Han,Jin, AU - Xia,Zhihui, AU - Liu,Pengcheng, AU - Li,Chunrong, AU - Wang,Yanyan, AU - Guo,Lequn, AU - Jiang,Guanghuai, AU - Zhai,Wenxue, Y1 - 2020/02/06/ PY - 2019/04/17/received PY - 2020/01/21/accepted PY - 2020/2/8/entrez PY - 2020/2/8/pubmed PY - 2020/11/20/medline SP - 2036 EP - 2036 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - The xa5 gene encodes a basal transcription factor (TFIIAγ) protein with wide spectrum resistance to bacterial blight caused by Xanthomonas oryzae pv. Oryzae (Xoo) in rice. It was only found in a few rice ecotypes, and the recessive characteristics limited its application in breeding. Here, we employed a TALEN-based technique to edit its dominant allelic TFIIAγ5 and obtained many mutant TFIIAγ5 genes. Most of them reduced rice susceptibility to varying degrees when the plants were challenged with the Xoo. In particular, the knocked-out TFIIAγ5 can reduce the rice susceptibility significantly, although it cannot reach the xa5-mediated resistance level, indicating TFIIAγ5 is a major component involved in disease susceptibility. In addition, the mutant encoding the protein with deletion of the 32nd amino acid or amino acid insertion between 32nd and 33rd site confers rice with the similar resistance to that of the knocked-out TFIIAγ5. Thus, the amino acids around 32nd site are also the important action sites of TFIIAγ5 besides the 39th amino acid previously reported. Moreover, the integration of xa5 into TFIIAγ5-knockout plants conferred them with a similar resistance as IRBB5, the rice variety containing the homozygous xa5 gene. Thus, TFIIAγ5 was not simply regarded as a resistant or a susceptible locus, as the substitution of amino acids might shift its functions. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32029874/TALEN_based_editing_of_TFIIAy5_changes_rice_response_to_Xanthomonas_oryzae_pv__Oryzae_ L2 - https://doi.org/10.1038/s41598-020-59052-w DB - PRIME DP - Unbound Medicine ER -