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Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation.
Anal Chem. 2020 04 07; 92(7):4807-4813.AC

Abstract

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death, which is demanding powerful diagnosis tools. Although the traditionally used serological biomarker alpha-fetoprotein (AFP) cannot meet the requirement of accurate diagnosis, its isoform alpha-fetoprotein L3 (AFP-L3) ratio in total AFP is emerging as a highly specific alternative. The routine electrophoretic blotting methods for analyzing AFP-L3 isoform ratio are reliable but often lack speediness, sensitivity, or accuracy. Herein, an elemental mass spectrometric strategy was established to simultaneously detect total AFP and AFP-L3 for the accurate HCC diagnosis. The metal isotopes inside colloidal gold nanoparticle (AuNP) and colloidal silver nanoparticle (AgNP) reporters were used to sensitively detect total AFP and AFP-L3, respectively. AFP-L3 and total AFP were accurately and simultaneously detected with the limits of detection (LODs) of 0.1 ng mL-1 and 0.2 ng mL-1, respectively. The proposed method was successfully validated in a series of human serum samples. The assay procedure was greatly simplified and less time-consuming for the AFP-L3 isoform ratio evaluation, when compared to clinical routine chromatographic/electrophoretic methods. Thanks to the highly multiplex ability of mass spectrometry, the proposed method provides great potential for the analysis of multiple isoforms of various disease biomarkers.

Authors+Show Affiliations

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.Analytical & Testing Center, Sichuan University, Chengdu 610064, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32030974

Citation

Li, Ziyan, et al. "Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation." Analytical Chemistry, vol. 92, no. 7, 2020, pp. 4807-4813.
Li Z, Li H, Deng D, et al. Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation. Anal Chem. 2020;92(7):4807-4813.
Li, Z., Li, H., Deng, D., Liu, R., & Lv, Y. (2020). Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation. Analytical Chemistry, 92(7), 4807-4813. https://doi.org/10.1021/acs.analchem.9b03995
Li Z, et al. Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation. Anal Chem. 2020 04 7;92(7):4807-4813. PubMed PMID: 32030974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mass Spectrometric Assay of Alpha-Fetoprotein Isoforms for Accurate Serological Evaluation. AU - Li,Ziyan, AU - Li,Hongmei, AU - Deng,Dongyan, AU - Liu,Rui, AU - Lv,Yi, Y1 - 2020/02/20/ PY - 2020/2/8/pubmed PY - 2021/1/26/medline PY - 2020/2/8/entrez SP - 4807 EP - 4813 JF - Analytical chemistry JO - Anal Chem VL - 92 IS - 7 N2 - Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death, which is demanding powerful diagnosis tools. Although the traditionally used serological biomarker alpha-fetoprotein (AFP) cannot meet the requirement of accurate diagnosis, its isoform alpha-fetoprotein L3 (AFP-L3) ratio in total AFP is emerging as a highly specific alternative. The routine electrophoretic blotting methods for analyzing AFP-L3 isoform ratio are reliable but often lack speediness, sensitivity, or accuracy. Herein, an elemental mass spectrometric strategy was established to simultaneously detect total AFP and AFP-L3 for the accurate HCC diagnosis. The metal isotopes inside colloidal gold nanoparticle (AuNP) and colloidal silver nanoparticle (AgNP) reporters were used to sensitively detect total AFP and AFP-L3, respectively. AFP-L3 and total AFP were accurately and simultaneously detected with the limits of detection (LODs) of 0.1 ng mL-1 and 0.2 ng mL-1, respectively. The proposed method was successfully validated in a series of human serum samples. The assay procedure was greatly simplified and less time-consuming for the AFP-L3 isoform ratio evaluation, when compared to clinical routine chromatographic/electrophoretic methods. Thanks to the highly multiplex ability of mass spectrometry, the proposed method provides great potential for the analysis of multiple isoforms of various disease biomarkers. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/32030974/Mass_Spectrometric_Assay_of_Alpha-Fetoprotein_Isoforms_for_Accurate_Serological_Evaluation L2 - https://doi.org/10.1021/acs.analchem.9b03995 DB - PRIME DP - Unbound Medicine ER -