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The impact of carbohydrate-active enzymes on mediating cell wall polysaccharide-tannin interactions in a wine-like matrix.
Food Res Int. 2020 03; 129:108889.FR

Abstract

Tannins are present in grape skins and seeds from where they are transferred into the must-wine matrix during the maceration stages of winemaking. However, tannin transfer is often incomplete. This could be due, among other reasons, to tannins becoming bound to grape cell wall polysaccharides, including soluble polymers, which are released during vinification and are present in high concentrations in the must/wine. The use of cell wall deconstructing enzymes offers the possibility of reducing these interactions, releasing more tannins into the final wine. The main aim of this study was to evaluate the optimal addition (individually, in combination or sequentially) of hydrolytic enzymes that would prevent tight polysaccharide-tannin associations. The use of comprehensive microarray polymer profiling (CoMPP) methodology provided key insights into how the enzyme treatments impacted the grape cell wall matrix and tannin binding. The results demonstrated that polygalacturonase + pectin-lyase promoted the highest release of tannins into solution.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Osete-Alcaraz A
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.
Gómez-Plaza E
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain. Electronic address: encarnag@um.es.
Martínez-Pérez P
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.
Weiller F
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland, 7602, South Africa.
Schückel J
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, DK-1001, Denmark; Glycospot, Thorvaldsensvej 40, B102, DK-1871 Frederiksberg C, Denmark(1).
Willats WGT
School of Agriculture, Food and Rural Development, Newcastle University, Newcastle-upon-Tyne, United Kingdom; Glycospot, Thorvaldsensvej 40, B102, DK-1871 Frederiksberg C, Denmark(1).
Moore JP
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland, 7602, South Africa.
Ros-García JM
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.
Bautista-Ortín AB
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30071 Murcia, Spain.

MeSH

Carbohydrate MetabolismCarbohydratesCell WallEnzymesFood HandlingFruitPolysaccharidesTanninsVitisWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32036932

Citation

Osete-Alcaraz, Andrea, et al. "The Impact of Carbohydrate-active Enzymes On Mediating Cell Wall Polysaccharide-tannin Interactions in a Wine-like Matrix." Food Research International (Ottawa, Ont.), vol. 129, 2020, p. 108889.
Osete-Alcaraz A, Gómez-Plaza E, Martínez-Pérez P, et al. The impact of carbohydrate-active enzymes on mediating cell wall polysaccharide-tannin interactions in a wine-like matrix. Food Res Int. 2020;129:108889.
Osete-Alcaraz, A., Gómez-Plaza, E., Martínez-Pérez, P., Weiller, F., Schückel, J., Willats, W. G. T., Moore, J. P., Ros-García, J. M., & Bautista-Ortín, A. B. (2020). The impact of carbohydrate-active enzymes on mediating cell wall polysaccharide-tannin interactions in a wine-like matrix. Food Research International (Ottawa, Ont.), 129, 108889. https://doi.org/10.1016/j.foodres.2019.108889
Osete-Alcaraz A, et al. The Impact of Carbohydrate-active Enzymes On Mediating Cell Wall Polysaccharide-tannin Interactions in a Wine-like Matrix. Food Res Int. 2020;129:108889. PubMed PMID: 32036932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The impact of carbohydrate-active enzymes on mediating cell wall polysaccharide-tannin interactions in a wine-like matrix. AU - Osete-Alcaraz,Andrea, AU - Gómez-Plaza,Encarna, AU - Martínez-Pérez,Pilar, AU - Weiller,Florent, AU - Schückel,Julia, AU - Willats,William G T, AU - Moore,John P, AU - Ros-García,José M, AU - Bautista-Ortín,Ana B, Y1 - 2019/12/14/ PY - 2019/03/28/received PY - 2019/09/28/revised PY - 2019/12/12/accepted PY - 2020/2/11/entrez PY - 2020/2/11/pubmed PY - 2021/3/2/medline KW - Cell wall KW - Cellulase KW - Pectin lyase KW - Polygalacturonase KW - Polysaccharides KW - Tannins KW - Wine SP - 108889 EP - 108889 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 129 N2 - Tannins are present in grape skins and seeds from where they are transferred into the must-wine matrix during the maceration stages of winemaking. However, tannin transfer is often incomplete. This could be due, among other reasons, to tannins becoming bound to grape cell wall polysaccharides, including soluble polymers, which are released during vinification and are present in high concentrations in the must/wine. The use of cell wall deconstructing enzymes offers the possibility of reducing these interactions, releasing more tannins into the final wine. The main aim of this study was to evaluate the optimal addition (individually, in combination or sequentially) of hydrolytic enzymes that would prevent tight polysaccharide-tannin associations. The use of comprehensive microarray polymer profiling (CoMPP) methodology provided key insights into how the enzyme treatments impacted the grape cell wall matrix and tannin binding. The results demonstrated that polygalacturonase + pectin-lyase promoted the highest release of tannins into solution. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/32036932/The_impact_of_carbohydrate_active_enzymes_on_mediating_cell_wall_polysaccharide_tannin_interactions_in_a_wine_like_matrix_ DB - PRIME DP - Unbound Medicine ER -