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Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent with Immunohistochemical Staining Intensity in Tenocytes.
Int J Mol Sci. 2020 Feb 06; 21(3)IJ

Abstract

Tenocytes are mechanosensitive cells intimately adapting their expression profile and hence, their phenotype to their respective mechanomilieu. The immunolocalization and expression intensity of tenogenic, anabolic and catabolic markers in tenocytes in response to in vitro mechanical loading have not been monitored by immunohistochemical staining (IHC). Thus, we investigated the association between IHC intensities, different stimulation frequencies, and tenogenic metabolism using a versatile mechanical stretcher. Primary tenocytes obtained from murine Achilles tendons were transferred to poly(dimethylsiloxane) (PDMS) elastomeric chamber. Chambers were cyclically stretched by 5% in uniaxial direction at a variation of tensile frequency (1 or 2 Hz) for 3 h. After stretching, cell physiology, IHC intensities of tendon-related markers, and protein level of the angiogenesis marker vascular endothelial growth factor (VEGF) were evaluated. Cell proliferation in tenocytes stimulated with 1 Hz stretch was significantly higher than with 2 Hz or without stretch, while 2 Hz stretch induced significantly reduced cell viability and proliferation with microscopically detectable apoptotic cell changes. The amount of scleraxis translocated into the nuclei and tenomodulin immunoreactivity of tenocytes treated with stretch were significantly higher than of non-stretched cells. The collagen type-1 expression level in tenocytes stretched at 1 Hz was significantly higher than in those cultivated with 2 Hz or without stretching, whereas the matrix metalloproteinase (MMP)-1 and MMP-13 immunoreactivities of cells stretched at 2 Hz were significantly higher than in those stimulated with 1 Hz or without stretching. The secreted VEGF-protein level of tenocytes stretched at 2 Hz was significantly higher than without stretching. Our IHC findings consistent with cell physiology suggest that appropriate stretching can reproduce in vitro short-term tenogenic anabolic/catabolic conditions and allow us to identify an anabolic stretching profile.

Authors+Show Affiliations

Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.Institute of Biological Information Processing: IBI-2, Forschungszentrum Jülich, 52425 Jülich, Germany.Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.Institute of Materials in Electrical Engineering 1 (IWE1) RWTH Aachen University, 52074 Aachen, Germany.Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.Institute of Biological Information Processing: IBI-2, Forschungszentrum Jülich, 52425 Jülich, Germany.Department of Anatomy and Cell Biology, Paracelsus Medical Private University, Nuremberg and Salzburg, 90419 Nuremberg, Germany.Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32041254

Citation

Kubo, Yusuke, et al. "Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent With Immunohistochemical Staining Intensity in Tenocytes." International Journal of Molecular Sciences, vol. 21, no. 3, 2020.
Kubo Y, Hoffmann B, Goltz K, et al. Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent with Immunohistochemical Staining Intensity in Tenocytes. Int J Mol Sci. 2020;21(3).
Kubo, Y., Hoffmann, B., Goltz, K., Schnakenberg, U., Jahr, H., Merkel, R., Schulze-Tanzil, G., Pufe, T., & Tohidnezhad, M. (2020). Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent with Immunohistochemical Staining Intensity in Tenocytes. International Journal of Molecular Sciences, 21(3). https://doi.org/10.3390/ijms21031082
Kubo Y, et al. Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent With Immunohistochemical Staining Intensity in Tenocytes. Int J Mol Sci. 2020 Feb 6;21(3) PubMed PMID: 32041254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent with Immunohistochemical Staining Intensity in Tenocytes. AU - Kubo,Yusuke, AU - Hoffmann,Bernd, AU - Goltz,Katja, AU - Schnakenberg,Uwe, AU - Jahr,Holger, AU - Merkel,Rudolf, AU - Schulze-Tanzil,Gundula, AU - Pufe,Thomas, AU - Tohidnezhad,Mersedeh, Y1 - 2020/02/06/ PY - 2019/12/30/received PY - 2020/01/24/revised PY - 2020/02/02/accepted PY - 2020/2/12/entrez PY - 2020/2/12/pubmed PY - 2020/2/12/medline KW - anabolism KW - catabolism KW - mechanical stretch KW - tenocyte JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 3 N2 - Tenocytes are mechanosensitive cells intimately adapting their expression profile and hence, their phenotype to their respective mechanomilieu. The immunolocalization and expression intensity of tenogenic, anabolic and catabolic markers in tenocytes in response to in vitro mechanical loading have not been monitored by immunohistochemical staining (IHC). Thus, we investigated the association between IHC intensities, different stimulation frequencies, and tenogenic metabolism using a versatile mechanical stretcher. Primary tenocytes obtained from murine Achilles tendons were transferred to poly(dimethylsiloxane) (PDMS) elastomeric chamber. Chambers were cyclically stretched by 5% in uniaxial direction at a variation of tensile frequency (1 or 2 Hz) for 3 h. After stretching, cell physiology, IHC intensities of tendon-related markers, and protein level of the angiogenesis marker vascular endothelial growth factor (VEGF) were evaluated. Cell proliferation in tenocytes stimulated with 1 Hz stretch was significantly higher than with 2 Hz or without stretch, while 2 Hz stretch induced significantly reduced cell viability and proliferation with microscopically detectable apoptotic cell changes. The amount of scleraxis translocated into the nuclei and tenomodulin immunoreactivity of tenocytes treated with stretch were significantly higher than of non-stretched cells. The collagen type-1 expression level in tenocytes stretched at 1 Hz was significantly higher than in those cultivated with 2 Hz or without stretching, whereas the matrix metalloproteinase (MMP)-1 and MMP-13 immunoreactivities of cells stretched at 2 Hz were significantly higher than in those stimulated with 1 Hz or without stretching. The secreted VEGF-protein level of tenocytes stretched at 2 Hz was significantly higher than without stretching. Our IHC findings consistent with cell physiology suggest that appropriate stretching can reproduce in vitro short-term tenogenic anabolic/catabolic conditions and allow us to identify an anabolic stretching profile. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32041254/Different_Frequency_of_Cyclic_Tensile_Strain_Relates_to_Anabolic/Catabolic_Conditions_Consistent_with_Immunohistochemical_Staining_Intensity_in_Tenocytes L2 - https://www.mdpi.com/resolver?pii=ijms21031082 DB - PRIME DP - Unbound Medicine ER -
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