Tags

Type your tag names separated by a space and hit enter

Ficus deltoidea extract down-regulates protein tyrosine phosphatase 1B expression in a rat model of type 2 diabetes mellitus: a new insight into its antidiabetic mechanism.
J Nutr Sci. 2020 01 20; 9:e2.JN

Abstract

Ficus deltoidea var. deltoidea Jack (FD) is a well-known plant used in Malay folklore medicine to lower blood glucose in diabetic patients. For further research of the antihyperglycemic mechanisms, the protein tyrosine phosphatase 1B (PTP1B)-inhibitory effect of FD was analysed both in vitro and in vivo. To optimise a method for FD extraction, water, 50, 70, 80, 90 and 95 % ethanol extracts were prepared and determined for their total phenolic and triterpene contents, and PTP1B-inhibition capacity. Among the tested extracts, 70 % ethanol FD extract showed a significant PTP1B inhibition (92·0 % inhibition at 200 µg/ml) and high phenolic and triterpene contents. A bioassay-guided fractionation of the 70 % ethanol extract led to the isolation of a new triterpene (3β,11β-dihydroxyolean-12-en-23-oic acid; F3) along with six known compounds. In vivo, 4 weeks' administration of 70 % ethanol FD extract (125, 250 and 500 mg/kg/d) to streptozotocin-nicotinamide-induced type 2 diabetic rats reversed the abnormal changes of blood glucose, insulin, total Hb, GLUT2, lipid profile, and oxidative stress in liver and pancreas. Moreover, FD reduced the mRNA expression of the key gluconeogenic enzymes (phosphoenolpyruvate carboxykinase and glucose 6-phosphatase) and restored insulin receptor and GLUT2 encoding gene (Slc2a2) expression. In addition, FD significantly down-regulated the hepatic PTP1B gene expression. These results revealed that FD could potentially improve insulin sensitivity, suppress hepatic glucose output and enhance glucose uptake in type 2 diabetes mellitus through down-regulation of PTP1B. Together, our findings give scientific evidence for the traditional use of FD as an antidiabetic agent.

Authors+Show Affiliations

Pharmacology Department, National Research Centre, Giza, Egypt.Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El-Einy Street, Cairo 11562, Egypt. Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, 6th October Campus, 12566, Egypt.Chemistry Department, College of Science, King Khalid University, Abha, Saudi Arabia. Biochemistry Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Pathology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.Pharmacology Department, National Research Centre, Giza, Egypt. Microbiology and Immunology Department, College of Medicine, University of Illinois, Chicago, IL, USA.Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr El-Einy Street, Cairo 11562, Egypt.Pharmacology Department, National Research Centre, Giza, Egypt.School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32042410

Citation

Abdel-Rahman, Rehab F., et al. "Ficus Deltoidea Extract Down-regulates Protein Tyrosine Phosphatase 1B Expression in a Rat Model of Type 2 Diabetes Mellitus: a New Insight Into Its Antidiabetic Mechanism." Journal of Nutritional Science, vol. 9, 2020, pp. e2.
Abdel-Rahman RF, Ezzat SM, Ogaly HA, et al. Ficus deltoidea extract down-regulates protein tyrosine phosphatase 1B expression in a rat model of type 2 diabetes mellitus: a new insight into its antidiabetic mechanism. J Nutr Sci. 2020;9:e2.
Abdel-Rahman, R. F., Ezzat, S. M., Ogaly, H. A., Abd-Elsalam, R. M., Hessin, A. F., Fekry, M. I., Mansour, D. F., & Mohamed, S. O. (2020). Ficus deltoidea extract down-regulates protein tyrosine phosphatase 1B expression in a rat model of type 2 diabetes mellitus: a new insight into its antidiabetic mechanism. Journal of Nutritional Science, 9, e2. https://doi.org/10.1017/jns.2019.40
Abdel-Rahman RF, et al. Ficus Deltoidea Extract Down-regulates Protein Tyrosine Phosphatase 1B Expression in a Rat Model of Type 2 Diabetes Mellitus: a New Insight Into Its Antidiabetic Mechanism. J Nutr Sci. 2020 01 20;9:e2. PubMed PMID: 32042410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ficus deltoidea extract down-regulates protein tyrosine phosphatase 1B expression in a rat model of type 2 diabetes mellitus: a new insight into its antidiabetic mechanism. AU - Abdel-Rahman,Rehab F, AU - Ezzat,Shahira M, AU - Ogaly,Hanan A, AU - Abd-Elsalam,Reham M, AU - Hessin,Alyaa F, AU - Fekry,Mostafa I, AU - Mansour,Dina F, AU - Mohamed,Shanaz O, Y1 - 2020/01/20/ PY - 2020/2/12/entrez PY - 2020/2/12/pubmed PY - 2021/6/5/medline KW - CAT, catalase KW - Dihydroxyolean-12-en-23-oic acid KW - FBG, fasting blood glucose KW - FD, Ficus deltoidea var. deltoidea Jack KW - Ficus deltoidea KW - G6Pase, glucose 6-phosphatase KW - GPx, glutathione peroxidase KW - GSH, reduced glutathione KW - Glucose 6-phosphatase KW - Glucose transporter-2 KW - MDA, malondialdehyde KW - MET, metformin KW - NA, nicotinamide KW - PEPCK, phosphoenolpyruvate carboxykinase KW - PTP, protein tyrosine phosphatase KW - Phosphoenolpyruvate carboxykinase KW - Protein tyrosine phosphatase 1B KW - SOD, superoxide dismutase KW - STZ, streptozotocin KW - Slc2a2, GLUT2 gene KW - T2DM, type 2 diabetes mellitus SP - e2 EP - e2 JF - Journal of nutritional science JO - J Nutr Sci VL - 9 N2 - Ficus deltoidea var. deltoidea Jack (FD) is a well-known plant used in Malay folklore medicine to lower blood glucose in diabetic patients. For further research of the antihyperglycemic mechanisms, the protein tyrosine phosphatase 1B (PTP1B)-inhibitory effect of FD was analysed both in vitro and in vivo. To optimise a method for FD extraction, water, 50, 70, 80, 90 and 95 % ethanol extracts were prepared and determined for their total phenolic and triterpene contents, and PTP1B-inhibition capacity. Among the tested extracts, 70 % ethanol FD extract showed a significant PTP1B inhibition (92·0 % inhibition at 200 µg/ml) and high phenolic and triterpene contents. A bioassay-guided fractionation of the 70 % ethanol extract led to the isolation of a new triterpene (3β,11β-dihydroxyolean-12-en-23-oic acid; F3) along with six known compounds. In vivo, 4 weeks' administration of 70 % ethanol FD extract (125, 250 and 500 mg/kg/d) to streptozotocin-nicotinamide-induced type 2 diabetic rats reversed the abnormal changes of blood glucose, insulin, total Hb, GLUT2, lipid profile, and oxidative stress in liver and pancreas. Moreover, FD reduced the mRNA expression of the key gluconeogenic enzymes (phosphoenolpyruvate carboxykinase and glucose 6-phosphatase) and restored insulin receptor and GLUT2 encoding gene (Slc2a2) expression. In addition, FD significantly down-regulated the hepatic PTP1B gene expression. These results revealed that FD could potentially improve insulin sensitivity, suppress hepatic glucose output and enhance glucose uptake in type 2 diabetes mellitus through down-regulation of PTP1B. Together, our findings give scientific evidence for the traditional use of FD as an antidiabetic agent. SN - 2048-6790 UR - https://www.unboundmedicine.com/medline/citation/32042410/Ficus_deltoidea_extract_down_regulates_protein_tyrosine_phosphatase_1B_expression_in_a_rat_model_of_type_2_diabetes_mellitus:_a_new_insight_into_its_antidiabetic_mechanism_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32042410/ DB - PRIME DP - Unbound Medicine ER -