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Auxin treatment of grapevine (Vitis vinifera L.) berries delays ripening onset by inhibiting cell expansion.
Plant Mol Biol. 2020 May; 103(1-2):91-111.PM

Abstract

KEY MESSAGE

Auxin treatment of grape (Vitis vinifera L.) berries delays ripening by inducing changes in gene expression and cell wall metabolism and could combat some deleterious climate change effects. Auxins are inhibitors of grape berry ripening and their application may be useful to delay harvest to counter effects of climate change. However, little is known about how this delay occurs. The expression of 1892 genes was significantly changed compared to the control during a 48 h time-course where the auxin 1-naphthaleneacetic acid (NAA) was applied to pre-veraison grape berries. Principal component analysis showed that the control and auxin-treated samples were most different at 3 h post-treatment when approximately three times more genes were induced than repressed by NAA. There was considerable cross-talk between hormone pathways, particularly between those of auxin and ethylene. Decreased expression of genes encoding putative cell wall catabolic enzymes (including those involved with pectin) and increased expression of putative cellulose synthases indicated that auxins may preserve cell wall structure. This was confirmed by immunochemical labelling of berry sections using antibodies that detect homogalacturonan (LM19) and methyl-esterified homogalacturonan (LM20) and by labelling with the CMB3a cellulose-binding module. Comparison of the auxin-induced changes in gene expression with the pattern of these genes during berry ripening showed that the effect on transcription is a mix of changes that may specifically alter the progress of berry development in a targeted manner and others that could be considered as non-specific changes. Several lines of evidence suggest that cell wall changes and associated berry softening are the first steps in ripening and that delaying cell expansion can delay ripening providing a possible mechanism for the observed auxin effects.

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Authors+Show Affiliations

Dal Santo S
Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
Tucker MR
School of Agriculture, Food and Wine, Level 4, Main WIC Building, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
Tan HT
School of Agriculture, Food and Wine, Level 4, Main WIC Building, University of Adelaide, Waite Campus, Urrbrae, SA, 5064, Australia.
Burbidge CA
CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA, 5064, Australia.
Fasoli M
E. & J. Gallo Winery, 600 Yosemite Blvd, Modesto, CA, 95354, USA.
Böttcher C
CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA, 5064, Australia.
Boss PK
CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA, 5064, Australia.
Pezzotti M
Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
Davies C
CSIRO Agriculture and Food, Locked Bag 2, Glen Osmond, SA, 5064, Australia. christopher.davies@csiro.au.

MeSH

Cell EnlargementCell WallFruitGene Expression Regulation, PlantIndoleacetic AcidsNaphthaleneacetic AcidsPlant CellsPlant Growth RegulatorsTimeVitis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32043226

Citation

Dal Santo, Silvia, et al. "Auxin Treatment of Grapevine (Vitis Vinifera L.) Berries Delays Ripening Onset By Inhibiting Cell Expansion." Plant Molecular Biology, vol. 103, no. 1-2, 2020, pp. 91-111.
Dal Santo S, Tucker MR, Tan HT, et al. Auxin treatment of grapevine (Vitis vinifera L.) berries delays ripening onset by inhibiting cell expansion. Plant Mol Biol. 2020;103(1-2):91-111.
Dal Santo, S., Tucker, M. R., Tan, H. T., Burbidge, C. A., Fasoli, M., Böttcher, C., Boss, P. K., Pezzotti, M., & Davies, C. (2020). Auxin treatment of grapevine (Vitis vinifera L.) berries delays ripening onset by inhibiting cell expansion. Plant Molecular Biology, 103(1-2), 91-111. https://doi.org/10.1007/s11103-020-00977-1
Dal Santo S, et al. Auxin Treatment of Grapevine (Vitis Vinifera L.) Berries Delays Ripening Onset By Inhibiting Cell Expansion. Plant Mol Biol. 2020;103(1-2):91-111. PubMed PMID: 32043226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Auxin treatment of grapevine (Vitis vinifera L.) berries delays ripening onset by inhibiting cell expansion. AU - Dal Santo,Silvia, AU - Tucker,Matthew R, AU - Tan,Hwei-Ting, AU - Burbidge,Crista A, AU - Fasoli,Marianna, AU - Böttcher,Christine, AU - Boss,Paul K, AU - Pezzotti,Mario, AU - Davies,Christopher, Y1 - 2020/02/10/ PY - 2019/11/05/received PY - 2020/02/04/accepted PY - 2020/2/12/pubmed PY - 2020/5/19/medline PY - 2020/2/12/entrez KW - Auxin KW - Cell walls KW - Gene expression KW - Grape ripening KW - Ripening KW - Transcriptome SP - 91 EP - 111 JF - Plant molecular biology JO - Plant Mol Biol VL - 103 IS - 1-2 N2 - KEY MESSAGE: Auxin treatment of grape (Vitis vinifera L.) berries delays ripening by inducing changes in gene expression and cell wall metabolism and could combat some deleterious climate change effects. Auxins are inhibitors of grape berry ripening and their application may be useful to delay harvest to counter effects of climate change. However, little is known about how this delay occurs. The expression of 1892 genes was significantly changed compared to the control during a 48 h time-course where the auxin 1-naphthaleneacetic acid (NAA) was applied to pre-veraison grape berries. Principal component analysis showed that the control and auxin-treated samples were most different at 3 h post-treatment when approximately three times more genes were induced than repressed by NAA. There was considerable cross-talk between hormone pathways, particularly between those of auxin and ethylene. Decreased expression of genes encoding putative cell wall catabolic enzymes (including those involved with pectin) and increased expression of putative cellulose synthases indicated that auxins may preserve cell wall structure. This was confirmed by immunochemical labelling of berry sections using antibodies that detect homogalacturonan (LM19) and methyl-esterified homogalacturonan (LM20) and by labelling with the CMB3a cellulose-binding module. Comparison of the auxin-induced changes in gene expression with the pattern of these genes during berry ripening showed that the effect on transcription is a mix of changes that may specifically alter the progress of berry development in a targeted manner and others that could be considered as non-specific changes. Several lines of evidence suggest that cell wall changes and associated berry softening are the first steps in ripening and that delaying cell expansion can delay ripening providing a possible mechanism for the observed auxin effects. SN - 1573-5028 UR - https://www.unboundmedicine.com/medline/citation/32043226/Auxin_treatment_of_grapevine__Vitis_vinifera_L___berries_delays_ripening_onset_by_inhibiting_cell_expansion_ L2 - https://doi.org/10.1007/s11103-020-00977-1 DB - PRIME DP - Unbound Medicine ER -