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Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.).
Genes (Basel). 2020 Feb 07; 11(2)G

Abstract

Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars' resistant to FSP.

Authors+Show Affiliations

Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China.Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China.Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China.Plant Protection Station of Songyang County, Lishui 323400, China.Weihai Academy of Agricultural Sciences, No. 411, Tongyi Road, Weihai 311300, China.Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China.School of Agricultural and Food Science, Zhejiang A&F University, Hangzhou 311300, China.Integrated Plant Protection Center, Lishui Institute of Agricultural and Forestry Sciences, 827 Liyang Stress, Lishui 323000, China.College of Ecology, Lishui University, Lishui, Zhejiang 323000, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32046085

Citation

Chen, Limin, et al. "Transcriptomic and Metabolomic Changes Triggered By Fusarium Solani in Common Bean (Phaseolus Vulgaris L.)." Genes, vol. 11, no. 2, 2020.
Chen L, Wu Q, He T, et al. Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.). Genes (Basel). 2020;11(2).
Chen, L., Wu, Q., He, T., Lan, J., Ding, L., Liu, T., Wu, Q., Pan, Y., & Chen, T. (2020). Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.). Genes, 11(2). https://doi.org/10.3390/genes11020177
Chen L, et al. Transcriptomic and Metabolomic Changes Triggered By Fusarium Solani in Common Bean (Phaseolus Vulgaris L.). Genes (Basel). 2020 Feb 7;11(2) PubMed PMID: 32046085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.). AU - Chen,Limin, AU - Wu,Quancong, AU - He,Tianjun, AU - Lan,Jianjun, AU - Ding,Li, AU - Liu,Tingfu, AU - Wu,Qianqian, AU - Pan,Yiming, AU - Chen,Tingting, Y1 - 2020/02/07/ PY - 2019/12/31/received PY - 2020/01/25/revised PY - 2020/02/03/accepted PY - 2020/2/13/entrez PY - 2020/2/13/pubmed PY - 2020/2/13/medline KW - Fusarium sp KW - common bean KW - fungus–plant interactions KW - induced response KW - metabolome KW - transcriptome JF - Genes JO - Genes (Basel) VL - 11 IS - 2 N2 - Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars' resistant to FSP. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/32046085/Transcriptomic_and_Metabolomic_Changes_Triggered_by_Fusarium_solani_in_Common_Bean_(Phaseolus_vulgaris_L.) L2 - http://www.mdpi.com/resolver?pii=genes11020177 DB - PRIME DP - Unbound Medicine ER -
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