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Diversity and Bioactive Potential of Actinobacteria from Unexplored Regions of Western Ghats, India.
Microorganisms. 2020 Feb 07; 8(2)M

Abstract

The search for novel bioactive metabolites continues to be of much importance around the world for pharmaceutical, agricultural, and industrial applications. Actinobacteria constitute one of the extremely interesting groups of microorganisms widely used as important biological contributors for a wide range of novel secondary metabolites. This study focused on the assessment of antimicrobial and antioxidant activity of crude extracts of actinobacterial strains. Western Ghats of India represents unique regions of biologically diverse areas called "hot spots". A total of 32 isolates were obtained from soil samples of different forest locations of Bisle Ghat and Virjapet situated in Western Ghats of Karnataka, India. The isolates were identified as species of Streptomyces, Nocardiopsis, and Nocardioides by cultural, morphological, and molecular studies. Based on preliminary screening, seven isolates were chosen for metabolites extraction and to determine antimicrobial activity qualitatively (disc diffusion method) and quantitatively (micro dilution method) and scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals. Crude extracts of all seven isolates exhibited fairly strong antibacterial activity towards MRSA strains (MRSA ATCC 33591, MRSA ATCC NR-46071, and MRSA ATCC 46171) with MIC varying from 15.62 to 125 μg/mL, whereas showed less inhibition potential towards Gram-negative bacteria Salmonella typhi (ATCC 25241) and Escherichia coli (ATCC 11775) with MIC of 125-500 μg/mL. The isolates namely S1A, SS5, SCA35, and SCA 11 inhibited Fusarium moniliforme (MTCC 6576) to a maximum extent with MIC ranging from 62.5 to 250 μg/mL. Crude extract of SCA 11 and SCA 13 exhibited potent scavenging activities against DPPH and ABTS radicals. The results from this study suggest that actinobacterial strains of Western Ghats are an excellent source of natural antimicrobial and antioxidant compounds. Further research investigations on purification, recovery, and structural characterization of the active compounds are to be carried out.

Authors+Show Affiliations

Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, India.Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, India.Microbial Strain Collection, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany.Institute of Microbiology, Technische Universität Braunschweig, 38106 Braunschweig, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32046111

Citation

Siddharth, Saket, et al. "Diversity and Bioactive Potential of Actinobacteria From Unexplored Regions of Western Ghats, India." Microorganisms, vol. 8, no. 2, 2020.
Siddharth S, Vittal RR, Wink J, et al. Diversity and Bioactive Potential of Actinobacteria from Unexplored Regions of Western Ghats, India. Microorganisms. 2020;8(2).
Siddharth, S., Vittal, R. R., Wink, J., & Steinert, M. (2020). Diversity and Bioactive Potential of Actinobacteria from Unexplored Regions of Western Ghats, India. Microorganisms, 8(2). https://doi.org/10.3390/microorganisms8020225
Siddharth S, et al. Diversity and Bioactive Potential of Actinobacteria From Unexplored Regions of Western Ghats, India. Microorganisms. 2020 Feb 7;8(2) PubMed PMID: 32046111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and Bioactive Potential of Actinobacteria from Unexplored Regions of Western Ghats, India. AU - Siddharth,Saket, AU - Vittal,Ravishankar Rai, AU - Wink,Joachim, AU - Steinert,Michael, Y1 - 2020/02/07/ PY - 2020/02/03/received PY - 2020/02/05/revised PY - 2020/02/06/accepted PY - 2020/2/13/entrez PY - 2020/2/13/pubmed PY - 2020/2/13/medline KW - MRSA KW - Western Ghats KW - actinobacteria KW - antimicrobial KW - antioxidants KW - diversity JF - Microorganisms JO - Microorganisms VL - 8 IS - 2 N2 - The search for novel bioactive metabolites continues to be of much importance around the world for pharmaceutical, agricultural, and industrial applications. Actinobacteria constitute one of the extremely interesting groups of microorganisms widely used as important biological contributors for a wide range of novel secondary metabolites. This study focused on the assessment of antimicrobial and antioxidant activity of crude extracts of actinobacterial strains. Western Ghats of India represents unique regions of biologically diverse areas called "hot spots". A total of 32 isolates were obtained from soil samples of different forest locations of Bisle Ghat and Virjapet situated in Western Ghats of Karnataka, India. The isolates were identified as species of Streptomyces, Nocardiopsis, and Nocardioides by cultural, morphological, and molecular studies. Based on preliminary screening, seven isolates were chosen for metabolites extraction and to determine antimicrobial activity qualitatively (disc diffusion method) and quantitatively (micro dilution method) and scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals. Crude extracts of all seven isolates exhibited fairly strong antibacterial activity towards MRSA strains (MRSA ATCC 33591, MRSA ATCC NR-46071, and MRSA ATCC 46171) with MIC varying from 15.62 to 125 μg/mL, whereas showed less inhibition potential towards Gram-negative bacteria Salmonella typhi (ATCC 25241) and Escherichia coli (ATCC 11775) with MIC of 125-500 μg/mL. The isolates namely S1A, SS5, SCA35, and SCA 11 inhibited Fusarium moniliforme (MTCC 6576) to a maximum extent with MIC ranging from 62.5 to 250 μg/mL. Crude extract of SCA 11 and SCA 13 exhibited potent scavenging activities against DPPH and ABTS radicals. The results from this study suggest that actinobacterial strains of Western Ghats are an excellent source of natural antimicrobial and antioxidant compounds. Further research investigations on purification, recovery, and structural characterization of the active compounds are to be carried out. SN - 2076-2607 UR - https://www.unboundmedicine.com/medline/citation/32046111/Diversity_and_Bioactive_Potential_of_Actinobacteria_from_Unexplored_Regions_of_Western_Ghats_India_ DB - PRIME DP - Unbound Medicine ER -
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