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Effects of tannase-converted green tea extract on skeletal muscle development.
BMC Complement Med Ther. 2020 Feb 11; 20(1):47.BC

Abstract

BACKGROUND

The aim of this study was to investigate the effect of tannase-converted green tea extract with a high (-)-epicatechin (EC), (-)-epigallocatechin (EGC), and gallic acid (GA) content on myotube density and fusion in normal and oxidative stress-induced C2C12 skeletal muscle cells. Although the use of green tea extract is considered beneficial, cellular and molecular mechanisms of action of tannase-converted green tea extracts that are used as potential muscle growth materials have not been thoroughly studied.

METHODS

This study used histological analysis and molecular biology techniques, and compared the results with those for AMPK activator 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside (AICAR) and green tea extracts.

RESULTS

The myotube density of normal and oxidative stress-induced C2C12 cells was significantly higher in the tannase-converted green tea extract-treated group than that observed in the other groups (normal cells: P < 0.01; oxidative stress-induced cells: P < 0.05). In addition, tannase-converted green tea extract and green tea extract treatments significantly upregulated the genetic expression of myogenin, Myf5, and MyoD (P < 0.05). The levels of AMP-activated protein kinase-α (AMPKα) and muscle RING-finger protein-1 (MuRF-1) in the tannase-converted green tea extract group were higher than those in the AICAR and green tea extract groups (P < 0.05).

CONCLUSIONS

Taken together, our findings describe that the high levels of EC, EGC, and GA in the tannase-converted green tea extract are attributable to the morphological changes in C2C12 cells and intercellular signaling pathways. Therefore, tannase-converted green tea extract can be used in the treatment of sarcopenia.

Authors+Show Affiliations

BK21 Plus, College of Health Science, Korea University, Seoul, 02841, Republic of Korea.Department of Food Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.Animal Center and Preclinical Evaluation Research Institute, Yonam College, Cheonan, 31005, Republic of Korea.BTC Corporation, #703, Technology Development Center, 705 Haean-ro, Sangnok-gu, Ansan-si, Gyeonggi-do, Republic of Korea.BTC Corporation, #703, Technology Development Center, 705 Haean-ro, Sangnok-gu, Ansan-si, Gyeonggi-do, Republic of Korea.Department of Food Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea. ypark@dongguk.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32046706

Citation

Hong, Ki-Bae, et al. "Effects of Tannase-converted Green Tea Extract On Skeletal Muscle Development." BMC Complementary Medicine and Therapies, vol. 20, no. 1, 2020, p. 47.
Hong KB, Lee HS, Hong JS, et al. Effects of tannase-converted green tea extract on skeletal muscle development. BMC Complement Med Ther. 2020;20(1):47.
Hong, K. B., Lee, H. S., Hong, J. S., Kim, D. H., Moon, J. M., & Park, Y. (2020). Effects of tannase-converted green tea extract on skeletal muscle development. BMC Complementary Medicine and Therapies, 20(1), 47. https://doi.org/10.1186/s12906-020-2827-7
Hong KB, et al. Effects of Tannase-converted Green Tea Extract On Skeletal Muscle Development. BMC Complement Med Ther. 2020 Feb 11;20(1):47. PubMed PMID: 32046706.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of tannase-converted green tea extract on skeletal muscle development. AU - Hong,Ki-Bae, AU - Lee,Hee-Seok, AU - Hong,Jeong Sup, AU - Kim,Dong Hyeon, AU - Moon,Joo Myung, AU - Park,Yooheon, Y1 - 2020/02/11/ PY - 2019/04/30/received PY - 2020/01/23/accepted PY - 2020/2/13/entrez PY - 2020/2/13/pubmed PY - 2020/8/18/medline KW - (−)-epicatechin KW - (−)-epigallocatechin KW - Sarcopenia KW - Skeletal muscle mass KW - Tannase-converted green tea extract SP - 47 EP - 47 JF - BMC complementary medicine and therapies JO - BMC Complement Med Ther VL - 20 IS - 1 N2 - BACKGROUND: The aim of this study was to investigate the effect of tannase-converted green tea extract with a high (-)-epicatechin (EC), (-)-epigallocatechin (EGC), and gallic acid (GA) content on myotube density and fusion in normal and oxidative stress-induced C2C12 skeletal muscle cells. Although the use of green tea extract is considered beneficial, cellular and molecular mechanisms of action of tannase-converted green tea extracts that are used as potential muscle growth materials have not been thoroughly studied. METHODS: This study used histological analysis and molecular biology techniques, and compared the results with those for AMPK activator 5-aminoimidazole-4-carboxamide-1-β-D-ribonucleoside (AICAR) and green tea extracts. RESULTS: The myotube density of normal and oxidative stress-induced C2C12 cells was significantly higher in the tannase-converted green tea extract-treated group than that observed in the other groups (normal cells: P < 0.01; oxidative stress-induced cells: P < 0.05). In addition, tannase-converted green tea extract and green tea extract treatments significantly upregulated the genetic expression of myogenin, Myf5, and MyoD (P < 0.05). The levels of AMP-activated protein kinase-α (AMPKα) and muscle RING-finger protein-1 (MuRF-1) in the tannase-converted green tea extract group were higher than those in the AICAR and green tea extract groups (P < 0.05). CONCLUSIONS: Taken together, our findings describe that the high levels of EC, EGC, and GA in the tannase-converted green tea extract are attributable to the morphological changes in C2C12 cells and intercellular signaling pathways. Therefore, tannase-converted green tea extract can be used in the treatment of sarcopenia. SN - 2662-7671 UR - https://www.unboundmedicine.com/medline/citation/32046706/Effects_of_tannase_converted_green_tea_extract_on_skeletal_muscle_development_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32046706/ DB - PRIME DP - Unbound Medicine ER -