Tags

Type your tag names separated by a space and hit enter

Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river.
Arch Virol. 2020 Apr; 165(4):853-863.AV

Abstract

Since its discovery, the first identified giant virus associated with amoebae, Acanthamoeba polyphaga mimivirus (APMV), has been rigorously studied to understand the structural and genomic complexity of this virus. In this work, we report the isolation and genomic characterization of a new mimivirus of lineage B, named "Borely moumouvirus". This new virus exhibits a structure and replicative cycle similar to those of other members of the family Mimiviridae. The genome of the new isolate is a linear double-strand DNA molecule of ~1.0 Mb, containing over 900 open reading frames. Genome annotation highlighted different translation system components encoded in the DNA of Borely moumouvirus, including aminoacyl-tRNA synthetases, translation factors, and tRNA molecules, in a distribution similar to that in other lineage B mimiviruses. Pan-genome analysis indicated an increase in the genetic arsenal of this group of viruses, showing that the family Mimiviridae is still expanding. Furthermore, phylogenetic analysis has shown that Borely moumouvirus is closely related to moumouvirus australiensis. This is the first mimivirus lineage B isolated from Brazilian territory to be characterized. Further prospecting studies are necessary for us to better understand the diversity of these viruses so a better classification system can be established.

Authors+Show Affiliations

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil.Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.Microbes, Evolution, Phylogeny and Infection (MEPHI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique-Hôpitaux de Marseille (AP-HM), Marseille, France. Institut Hospitalo-Universitaire (IHU)-Méditerranée Infection, 19-21 boulevard Jean moulin, 13005, Marseille, France.Microbes, Evolution, Phylogeny and Infection (MEPHI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique-Hôpitaux de Marseille (AP-HM), Marseille, France. Institut Hospitalo-Universitaire (IHU)-Méditerranée Infection, 19-21 boulevard Jean moulin, 13005, Marseille, France.Microbes, Evolution, Phylogeny and Infection (MEPHI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique-Hôpitaux de Marseille (AP-HM), Marseille, France. bernard.la-scola@univ-amu.fr. Institut Hospitalo-Universitaire (IHU)-Méditerranée Infection, 19-21 boulevard Jean moulin, 13005, Marseille, France. bernard.la-scola@univ-amu.fr.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. jonatas.abrahao@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32052196

Citation

Dos Santos Silva, Ludmila Karen, et al. "Isolation and Genomic Characterization of a New Mimivirus of Lineage B From a Brazilian River." Archives of Virology, vol. 165, no. 4, 2020, pp. 853-863.
Dos Santos Silva LK, Rodrigues RAL, Dos Santos Pereira Andrade AC, et al. Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river. Arch Virol. 2020;165(4):853-863.
Dos Santos Silva, L. K., Rodrigues, R. A. L., Dos Santos Pereira Andrade, A. C., Hikida, H., Andreani, J., Levasseur, A., La Scola, B., & Abrahão, J. S. (2020). Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river. Archives of Virology, 165(4), 853-863. https://doi.org/10.1007/s00705-020-04542-5
Dos Santos Silva LK, et al. Isolation and Genomic Characterization of a New Mimivirus of Lineage B From a Brazilian River. Arch Virol. 2020;165(4):853-863. PubMed PMID: 32052196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and genomic characterization of a new mimivirus of lineage B from a Brazilian river. AU - Dos Santos Silva,Ludmila Karen, AU - Rodrigues,Rodrigo Araújo Lima, AU - Dos Santos Pereira Andrade,Ana Cláudia, AU - Hikida,Hiroyuki, AU - Andreani,Julien, AU - Levasseur,Anthony, AU - La Scola,Bernard, AU - Abrahão,Jônatas Santos, Y1 - 2020/02/12/ PY - 2019/09/16/received PY - 2020/01/08/accepted PY - 2020/2/14/pubmed PY - 2020/4/9/medline PY - 2020/2/14/entrez SP - 853 EP - 863 JF - Archives of virology JO - Arch. Virol. VL - 165 IS - 4 N2 - Since its discovery, the first identified giant virus associated with amoebae, Acanthamoeba polyphaga mimivirus (APMV), has been rigorously studied to understand the structural and genomic complexity of this virus. In this work, we report the isolation and genomic characterization of a new mimivirus of lineage B, named "Borely moumouvirus". This new virus exhibits a structure and replicative cycle similar to those of other members of the family Mimiviridae. The genome of the new isolate is a linear double-strand DNA molecule of ~1.0 Mb, containing over 900 open reading frames. Genome annotation highlighted different translation system components encoded in the DNA of Borely moumouvirus, including aminoacyl-tRNA synthetases, translation factors, and tRNA molecules, in a distribution similar to that in other lineage B mimiviruses. Pan-genome analysis indicated an increase in the genetic arsenal of this group of viruses, showing that the family Mimiviridae is still expanding. Furthermore, phylogenetic analysis has shown that Borely moumouvirus is closely related to moumouvirus australiensis. This is the first mimivirus lineage B isolated from Brazilian territory to be characterized. Further prospecting studies are necessary for us to better understand the diversity of these viruses so a better classification system can be established. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/32052196/Isolation_and_genomic_characterization_of_a_new_mimivirus_of_lineage_B_from_a_Brazilian_river_ L2 - https://dx.doi.org/10.1007/s00705-020-04542-5 DB - PRIME DP - Unbound Medicine ER -