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Candidatus Neoehrlichia mikurensis is widespread in questing Ixodes ricinus ticks in the Czech Republic.
Ticks Tick Borne Dis. 2020 05; 11(3):101371.TT

Abstract

Candidatus Neoehrlichia mikurensis, the causative agent of tick-borne "neoehrlichiosis" has recently been reported in humans, mammals and ticks in Europe. The aim of this study was to map the distribution of this bacterium in questing ticks in the Czech Republic. A total of 13,325 Ixodes ricinus including 445 larvae, 5270 nymphs and 7610 adults were collected from vegetation by flagging in 140 Czech towns and villages from every region of the Czech Republic. The ticks were pooled into 2665 groups of 5 individuals respecting life stage or sex and tested for the presence of Ca. Neoehrlichia mikurensis by conventional PCR targeting of the groEL gene. The bacterium was detected in 533/2665 pools and 125/140 areas screened, showing an overall estimated prevalence of 4.4 % in ticks of all life stages. Phylogenetic analysis revealed only small genetic diversity among the strains found. Two pools of questing larvae tested positive, suggesting transovarial transmission. According to this study, Ca. Neoehrlichia mikurensis is another tick-borne pathogen widespread in I. ricinus ticks in the Czech Republic.

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Authors+Show Affiliations

Ondruš J
CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic. Electronic address: jaroslavondrus@seznam.cz.
Balážová A
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic. Electronic address: Aja.Balazova@seznam.cz.
Baláž V
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic. Electronic address: balazv@vfu.cz.
Zechmeisterová K
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic. Electronic address: zechmeisterova.k@gmail.com.
Novobilský A
Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic. Electronic address: novobilsky@vri.cz.
Široký P
CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1946/1, 612 42, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42, Brno, Czech Republic. Electronic address: sirokyp@vfu.cz.

MeSH

AnaplasmataceaeAnimalsCzech RepublicFemaleGenetic VariationIxodesLarvaMaleNymph

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32057703

Citation

Ondruš, Jaroslav, et al. "Candidatus Neoehrlichia Mikurensis Is Widespread in Questing Ixodes Ricinus Ticks in the Czech Republic." Ticks and Tick-borne Diseases, vol. 11, no. 3, 2020, p. 101371.
Ondruš J, Balážová A, Baláž V, et al. Candidatus Neoehrlichia mikurensis is widespread in questing Ixodes ricinus ticks in the Czech Republic. Ticks Tick Borne Dis. 2020;11(3):101371.
Ondruš, J., Balážová, A., Baláž, V., Zechmeisterová, K., Novobilský, A., & Široký, P. (2020). Candidatus Neoehrlichia mikurensis is widespread in questing Ixodes ricinus ticks in the Czech Republic. Ticks and Tick-borne Diseases, 11(3), 101371. https://doi.org/10.1016/j.ttbdis.2020.101371
Ondruš J, et al. Candidatus Neoehrlichia Mikurensis Is Widespread in Questing Ixodes Ricinus Ticks in the Czech Republic. Ticks Tick Borne Dis. 2020;11(3):101371. PubMed PMID: 32057703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Candidatus Neoehrlichia mikurensis is widespread in questing Ixodes ricinus ticks in the Czech Republic. AU - Ondruš,Jaroslav, AU - Balážová,Alena, AU - Baláž,Vojtech, AU - Zechmeisterová,Kristína, AU - Novobilský,Adam, AU - Široký,Pavel, Y1 - 2020/01/09/ PY - 2019/07/23/received PY - 2020/01/03/revised PY - 2020/01/08/accepted PY - 2020/2/15/pubmed PY - 2021/3/17/medline PY - 2020/2/15/entrez KW - Anaplasmataceae KW - Candidatus Neoehrlichia mikurensis KW - Czech Republic KW - Ixodes ricinus KW - Tick-borne disease SP - 101371 EP - 101371 JF - Ticks and tick-borne diseases JO - Ticks Tick Borne Dis VL - 11 IS - 3 N2 - Candidatus Neoehrlichia mikurensis, the causative agent of tick-borne "neoehrlichiosis" has recently been reported in humans, mammals and ticks in Europe. The aim of this study was to map the distribution of this bacterium in questing ticks in the Czech Republic. A total of 13,325 Ixodes ricinus including 445 larvae, 5270 nymphs and 7610 adults were collected from vegetation by flagging in 140 Czech towns and villages from every region of the Czech Republic. The ticks were pooled into 2665 groups of 5 individuals respecting life stage or sex and tested for the presence of Ca. Neoehrlichia mikurensis by conventional PCR targeting of the groEL gene. The bacterium was detected in 533/2665 pools and 125/140 areas screened, showing an overall estimated prevalence of 4.4 % in ticks of all life stages. Phylogenetic analysis revealed only small genetic diversity among the strains found. Two pools of questing larvae tested positive, suggesting transovarial transmission. According to this study, Ca. Neoehrlichia mikurensis is another tick-borne pathogen widespread in I. ricinus ticks in the Czech Republic. SN - 1877-9603 UR - https://www.unboundmedicine.com/medline/citation/32057703/Candidatus_Neoehrlichia_mikurensis_is_widespread_in_questing_Ixodes_ricinus_ticks_in_the_Czech_Republic_ DB - PRIME DP - Unbound Medicine ER -