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Identification of pmrB mutations as putative mechanism for colistin resistance in A. baumannii strains isolated after in vivo colistin exposure.
Microb Pathog. 2020 Feb 11; 142:104058.MP

Abstract

Colistin resistance among extensively-resistant Acinetobacter baumannii isolates is a serious health-care problem. Alterations in PmrA-PmrB two-component system have been associated with resistance to colistin. We investigated three pairs of colistin-susceptible and colistin-resistant A. baumannii, sequentially isolated from three patients before and after colistin treatment, respectively. The pmrA and pmrB genes were sequenced by Sanger method. Amino acidic positions and their effect on protein were predicted by InterPro and PROVEAN tools. Expression of pmrA, pmrB and pmrC genes was assessed by semi-quantitative reverse transcription-PCR (qRT-PCR). We found three different nonsynonymous substitutions P233T, E301G and L168K in pmrB coding region, each one in a different colistin resistance strain. The E301G and L168K substitutions represent novel mutations in pmrB, not previously described. Relative expression of pmrA, pmrB and pmrC mRNA increased in all colistin resistant strains. In our study, pmrB substitutions were associated with pmrC over-expression and colistin resistance. Further studies are necessary to understand their impact on modification of lipid A components.

Authors+Show Affiliations

Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy. Electronic address: nadiamarascio@gmail.com.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.Institute for Experimental Veterinary Medicine of Southern Italy, 88100, Catanzaro, Italy.Institute for Experimental Veterinary Medicine of Southern Italy, 88100, Catanzaro, Italy.Department of Medical and Surgical Sciences, Unit of Infectious and Tropical Diseases, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Medical and Surgical Sciences, Unit of Infectious and Tropical Diseases, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Medical and Surgical Sciences, Unit of Infectious and Tropical Diseases, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.Department of Health Sciences, Unit of Clinical Microbiology, "Magna Graecia" University, 88100, Catanzaro, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32058026

Citation

Marano, Vito, et al. "Identification of pmrB Mutations as Putative Mechanism for Colistin Resistance in A. Baumannii Strains Isolated After in Vivo Colistin Exposure." Microbial Pathogenesis, vol. 142, 2020, p. 104058.
Marano V, Marascio N, Pavia G, et al. Identification of pmrB mutations as putative mechanism for colistin resistance in A. baumannii strains isolated after in vivo colistin exposure. Microb Pathog. 2020;142:104058.
Marano, V., Marascio, N., Pavia, G., Lamberti, A. G., Quirino, A., Musarella, R., Casalinuovo, F., Mazzitelli, M., Trecarichi, E. M., Torti, C., Matera, G., & Liberto, M. C. (2020). Identification of pmrB mutations as putative mechanism for colistin resistance in A. baumannii strains isolated after in vivo colistin exposure. Microbial Pathogenesis, 142, 104058. https://doi.org/10.1016/j.micpath.2020.104058
Marano V, et al. Identification of pmrB Mutations as Putative Mechanism for Colistin Resistance in A. Baumannii Strains Isolated After in Vivo Colistin Exposure. Microb Pathog. 2020 Feb 11;142:104058. PubMed PMID: 32058026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of pmrB mutations as putative mechanism for colistin resistance in A. baumannii strains isolated after in vivo colistin exposure. AU - Marano,Vito, AU - Marascio,Nadia, AU - Pavia,Grazia, AU - Lamberti,Angelo G, AU - Quirino,Angela, AU - Musarella,Rosanna, AU - Casalinuovo,Francesco, AU - Mazzitelli,Maria, AU - Trecarichi,Enrico M, AU - Torti,Carlo, AU - Matera,Giovanni, AU - Liberto,Maria Carla, Y1 - 2020/02/11/ PY - 2019/11/21/received PY - 2020/01/20/revised PY - 2020/02/10/accepted PY - 2020/2/15/pubmed PY - 2020/2/15/medline PY - 2020/2/15/entrez KW - A. baumannii KW - Colistin resistance KW - pmrB mutations SP - 104058 EP - 104058 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 142 N2 - Colistin resistance among extensively-resistant Acinetobacter baumannii isolates is a serious health-care problem. Alterations in PmrA-PmrB two-component system have been associated with resistance to colistin. We investigated three pairs of colistin-susceptible and colistin-resistant A. baumannii, sequentially isolated from three patients before and after colistin treatment, respectively. The pmrA and pmrB genes were sequenced by Sanger method. Amino acidic positions and their effect on protein were predicted by InterPro and PROVEAN tools. Expression of pmrA, pmrB and pmrC genes was assessed by semi-quantitative reverse transcription-PCR (qRT-PCR). We found three different nonsynonymous substitutions P233T, E301G and L168K in pmrB coding region, each one in a different colistin resistance strain. The E301G and L168K substitutions represent novel mutations in pmrB, not previously described. Relative expression of pmrA, pmrB and pmrC mRNA increased in all colistin resistant strains. In our study, pmrB substitutions were associated with pmrC over-expression and colistin resistance. Further studies are necessary to understand their impact on modification of lipid A components. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/32058026/Identification_of_pmrB_mutations_as_putative_mechanism_for_colistin_resistance_in_A__baumannii_strains_isolated_after_in_vivo_colistin_exposure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(19)32042-X DB - PRIME DP - Unbound Medicine ER -
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