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Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration.
Antonie Van Leeuwenhoek. 2020 Jun; 113(6):825-837.AV

Abstract

Filamentous Actinobacteria are multicellular bacteria with linear replicons. Kitasatospora viridifaciens DSM 40239 contains a linear 7.8 Mb chromosome and an autonomously replicating plasmid KVP1 of 1.7 Mb. Here we show that lysozyme-induced protoplast formation of the multinucleated mycelium of K. viridifaciens drives morphological diversity. Characterisation and sequencing of an individual revertant colony that had lost the ability to differentiate revealed that the strain had not only lost most of KVP1 but also carried deletions in the right arm of the chromosome. Strikingly, the deletion sites were preceded by insertion sequence elements, suggesting that the rearrangements may have been caused by replicative transposition and homologous recombination between both replicons. These data indicate that protoplast formation is a stressful process that can lead to profound genetic changes.

Authors+Show Affiliations

Molecular Biotechnology, Institute of Biology, Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.Molecular Biotechnology, Institute of Biology, Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.Molecular Biotechnology, Institute of Biology, Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands.Molecular Biotechnology, Institute of Biology, Leiden University, P.O. Box 9505, 2300 RA, Leiden, The Netherlands. D.Claessen@biology.leidenuniv.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32060816

Citation

Ramijan, Karina, et al. "Genome Rearrangements and Megaplasmid Loss in the Filamentous Bacterium Kitasatospora Viridifaciens Are Associated With Protoplast Formation and Regeneration." Antonie Van Leeuwenhoek, vol. 113, no. 6, 2020, pp. 825-837.
Ramijan K, Zhang Z, van Wezel GP, et al. Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration. Antonie Van Leeuwenhoek. 2020;113(6):825-837.
Ramijan, K., Zhang, Z., van Wezel, G. P., & Claessen, D. (2020). Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration. Antonie Van Leeuwenhoek, 113(6), 825-837. https://doi.org/10.1007/s10482-020-01393-7
Ramijan K, et al. Genome Rearrangements and Megaplasmid Loss in the Filamentous Bacterium Kitasatospora Viridifaciens Are Associated With Protoplast Formation and Regeneration. Antonie Van Leeuwenhoek. 2020;113(6):825-837. PubMed PMID: 32060816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome rearrangements and megaplasmid loss in the filamentous bacterium Kitasatospora viridifaciens are associated with protoplast formation and regeneration. AU - Ramijan,Karina, AU - Zhang,Zheren, AU - van Wezel,Gilles P, AU - Claessen,Dennis, Y1 - 2020/02/14/ PY - 2019/08/29/received PY - 2020/02/05/accepted PY - 2020/2/16/pubmed PY - 2020/2/16/medline PY - 2020/2/16/entrez KW - Actinobacteria KW - Genetic instability KW - Heterogeneity KW - Insertion sequence KW - Protoplast SP - 825 EP - 837 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 113 IS - 6 N2 - Filamentous Actinobacteria are multicellular bacteria with linear replicons. Kitasatospora viridifaciens DSM 40239 contains a linear 7.8 Mb chromosome and an autonomously replicating plasmid KVP1 of 1.7 Mb. Here we show that lysozyme-induced protoplast formation of the multinucleated mycelium of K. viridifaciens drives morphological diversity. Characterisation and sequencing of an individual revertant colony that had lost the ability to differentiate revealed that the strain had not only lost most of KVP1 but also carried deletions in the right arm of the chromosome. Strikingly, the deletion sites were preceded by insertion sequence elements, suggesting that the rearrangements may have been caused by replicative transposition and homologous recombination between both replicons. These data indicate that protoplast formation is a stressful process that can lead to profound genetic changes. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/32060816/Genome_rearrangements_and_megaplasmid_loss_in_the_filamentous_bacterium_Kitasatospora_viridifaciens_are_associated_with_protoplast_formation_and_regeneration L2 - https://doi.org/10.1007/s10482-020-01393-7 DB - PRIME DP - Unbound Medicine ER -
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