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Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae).
J Insect Sci. 2020 Jan 01; 20(1)JI

Abstract

The vanilloid-type transient receptor potential (TRPV) channel is reported to be the molecular target of the commercial insecticide pymetrozine, which specifically disrupts the feeding of plant sap-sucking insects. However, the functions of TRPV channels in plant sap-sucking insects have not been fully elucidated. In the present study, RNA interference was used to investigate the effects of the knockdown of TRPV genes (Nan and Iav) on the mortality, locomotion, and feeding behavior of an important plant-feeding insect pest in rice, the brown planthopper, Nilaparvata lugens. Injecting dsRNA of Nan and Iav into fourth-instar nymphs significantly knocked down the target genes. The injection of dsNan or dsIav did not affect any morphological phenotype (including leg extension) of N. lugens nymphs and adults. Knockdown of Nan or Iav resulted in significantly decreased climbing activity against top plants but did not influence the leg-griping strength of adults. Knockdown of Nan resulted in a significantly elevated mortality of N. lugens in the observation period of 7 d after injection, whereas no significant difference in survival rates 7 d after injection was found between dsIav-injected and dsGFP-injected insects. Electropenetrographic (EPG) recordings indicated that knockdown of Nan and Iav reduced the ingestion activity in the rice phloem tissues of N. lugens. Knockdown of Nan and Iav significantly reduced the amount of honeydew excreted by N. lugens. Our findings indicated a relationship between TRPV and N. lugens locomotion and feeding behavior, which may help to fully elucidate the functions of TRPV in insects.

Authors+Show Affiliations

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32061084

Citation

Zhu, Jinghua, et al. "Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata Lugens (Hemiptera: Delphacidae)." Journal of Insect Science (Online), vol. 20, no. 1, 2020.
Zhu J, Liu X, Zhu K, et al. Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae). J Insect Sci. 2020;20(1).
Zhu, J., Liu, X., Zhu, K., Zhou, H., Li, L., Li, Z., Qin, W., & He, Y. (2020). Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae). Journal of Insect Science (Online), 20(1). https://doi.org/10.1093/jisesa/ieaa002
Zhu J, et al. Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata Lugens (Hemiptera: Delphacidae). J Insect Sci. 2020 Jan 1;20(1) PubMed PMID: 32061084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of TRPV Genes Affects the Locomotion and Feeding Behavior of Nilaparvata lugens (Hemiptera: Delphacidae). AU - Zhu,Jinghua, AU - Liu,Xiaoqing, AU - Zhu,Kunmiao, AU - Zhou,Hanyu, AU - Li,Liang, AU - Li,Zengxin, AU - Qin,Weiwei, AU - He,Yueping, PY - 2019/11/08/received PY - 2020/2/16/entrez PY - 2020/2/16/pubmed PY - 2020/2/28/medline KW - Nilaparvata lugens KW - RNAi KW - electropenetrography KW - feeding behavior KW - transient receptor potential JF - Journal of insect science (Online) JO - J. Insect Sci. VL - 20 IS - 1 N2 - The vanilloid-type transient receptor potential (TRPV) channel is reported to be the molecular target of the commercial insecticide pymetrozine, which specifically disrupts the feeding of plant sap-sucking insects. However, the functions of TRPV channels in plant sap-sucking insects have not been fully elucidated. In the present study, RNA interference was used to investigate the effects of the knockdown of TRPV genes (Nan and Iav) on the mortality, locomotion, and feeding behavior of an important plant-feeding insect pest in rice, the brown planthopper, Nilaparvata lugens. Injecting dsRNA of Nan and Iav into fourth-instar nymphs significantly knocked down the target genes. The injection of dsNan or dsIav did not affect any morphological phenotype (including leg extension) of N. lugens nymphs and adults. Knockdown of Nan or Iav resulted in significantly decreased climbing activity against top plants but did not influence the leg-griping strength of adults. Knockdown of Nan resulted in a significantly elevated mortality of N. lugens in the observation period of 7 d after injection, whereas no significant difference in survival rates 7 d after injection was found between dsIav-injected and dsGFP-injected insects. Electropenetrographic (EPG) recordings indicated that knockdown of Nan and Iav reduced the ingestion activity in the rice phloem tissues of N. lugens. Knockdown of Nan and Iav significantly reduced the amount of honeydew excreted by N. lugens. Our findings indicated a relationship between TRPV and N. lugens locomotion and feeding behavior, which may help to fully elucidate the functions of TRPV in insects. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/32061084/Knockdown_of_TRPV_Genes_Affects_the_Locomotion_and_Feeding_Behavior_of_Nilaparvata_lugens_(Hemiptera:_Delphacidae) L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1093/jisesa/ieaa002 DB - PRIME DP - Unbound Medicine ER -