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Muscle vasodilator response via potential adrenaline secretion to L-cysteine microinjected in rostral ventrolateral medulla of rats.
Auton Neurosci. 2020 03; 224:102644.AN

Abstract

We previously found that the thiol amino acid L-cysteine microinjected into rat medullary autonomic areas produces changes in arterial blood pressure (AP) via ionotropic excitatory amino acid receptors (iEAAr), but its effect on vascular beds is still unknown. Rostral ventrolateral medulla (RVLM) pressor area includes adrenal and lumbar presympathetic neurons which activation could cause opposite muscle vascular responses: vasodilation versus vasoconstriction. However, there are few data on the vasodilator response in rats. Ionotropic EAAr activation alone with L-cysteine may be effective to differentiate RVLM sites for those opposite responses. To test it, muscle blood flow responses to L-cysteine were mapped in the ventrolateral medulla (VLM) of rats. In anesthetized rats with a cranial window above the VLM, hindquarter flow (HQF) and AP were monitored, providing hindquarter resistance (HQR) by dividing AP by HQF. L-Cysteine mapping in VLM including caudal depressor vasodilator area defined with L-glutamate showed HQR responses in parallel to AP responses, suggesting the importance of iEAAr in muscle vascular regulation. Microinjections of L-cysteine into RVLM succeeded to detect sites of slower muscle vasodilation and blockade of peripheral β-adrenoceptors abolished this response, indicating potential adrenaline secretion. Although there was no functional topography, the iEAAr activation alone with L-cysteine can differentiate sites of muscle vasodilation from vasoconstriction in rat RVLM. The neuromodulator candidate L-cysteine is a useful tool when chemical stimulation of iEAAr is required.

Authors+Show Affiliations

Department of Physiology II, Graduate School of Biomedical and Health Sciences (Medicine), Hiroshima University, Japan; Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.. Electronic address: yumitake@hiroshima-u.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32062594

Citation

Takemoto, Yumi. "Muscle Vasodilator Response Via Potential Adrenaline Secretion to L-cysteine Microinjected in Rostral Ventrolateral Medulla of Rats." Autonomic Neuroscience : Basic & Clinical, vol. 224, 2020, p. 102644.
Takemoto Y. Muscle vasodilator response via potential adrenaline secretion to L-cysteine microinjected in rostral ventrolateral medulla of rats. Auton Neurosci. 2020;224:102644.
Takemoto, Y. (2020). Muscle vasodilator response via potential adrenaline secretion to L-cysteine microinjected in rostral ventrolateral medulla of rats. Autonomic Neuroscience : Basic & Clinical, 224, 102644. https://doi.org/10.1016/j.autneu.2020.102644
Takemoto Y. Muscle Vasodilator Response Via Potential Adrenaline Secretion to L-cysteine Microinjected in Rostral Ventrolateral Medulla of Rats. Auton Neurosci. 2020;224:102644. PubMed PMID: 32062594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muscle vasodilator response via potential adrenaline secretion to L-cysteine microinjected in rostral ventrolateral medulla of rats. A1 - Takemoto,Yumi, Y1 - 2020/01/30/ PY - 2019/10/17/received PY - 2020/01/27/revised PY - 2020/01/27/accepted PY - 2020/2/18/pubmed PY - 2020/2/18/medline PY - 2020/2/17/entrez KW - Adrenaline secretion KW - Ionotropic excitatory amino acid receptors KW - L-Cysteine KW - Muscle vasodilation KW - RVLM KW - Rats SP - 102644 EP - 102644 JF - Autonomic neuroscience : basic & clinical JO - Auton Neurosci VL - 224 N2 - We previously found that the thiol amino acid L-cysteine microinjected into rat medullary autonomic areas produces changes in arterial blood pressure (AP) via ionotropic excitatory amino acid receptors (iEAAr), but its effect on vascular beds is still unknown. Rostral ventrolateral medulla (RVLM) pressor area includes adrenal and lumbar presympathetic neurons which activation could cause opposite muscle vascular responses: vasodilation versus vasoconstriction. However, there are few data on the vasodilator response in rats. Ionotropic EAAr activation alone with L-cysteine may be effective to differentiate RVLM sites for those opposite responses. To test it, muscle blood flow responses to L-cysteine were mapped in the ventrolateral medulla (VLM) of rats. In anesthetized rats with a cranial window above the VLM, hindquarter flow (HQF) and AP were monitored, providing hindquarter resistance (HQR) by dividing AP by HQF. L-Cysteine mapping in VLM including caudal depressor vasodilator area defined with L-glutamate showed HQR responses in parallel to AP responses, suggesting the importance of iEAAr in muscle vascular regulation. Microinjections of L-cysteine into RVLM succeeded to detect sites of slower muscle vasodilation and blockade of peripheral β-adrenoceptors abolished this response, indicating potential adrenaline secretion. Although there was no functional topography, the iEAAr activation alone with L-cysteine can differentiate sites of muscle vasodilation from vasoconstriction in rat RVLM. The neuromodulator candidate L-cysteine is a useful tool when chemical stimulation of iEAAr is required. SN - 1872-7484 UR - https://www.unboundmedicine.com/medline/citation/32062594/Muscle_vasodilator_response_via_potential_adrenaline_secretion_to_L-cysteine_microinjected_in_rostral_ventrolateral_medulla_of_rats L2 - https://linkinghub.elsevier.com/retrieve/pii/S1566-0702(19)30253-X DB - PRIME DP - Unbound Medicine ER -
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