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Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in Caco-2 cells.
Food Funct. 2020 Feb 26; 11(2):1835-1844.FF

Abstract

The present study attempted to evaluate the mechanism of action and bioactivity of mulberry leaf polyphenols (MLPs) in type-2 diabetes prevention via inhibition of disaccharidase and glucose transport. MLPs were purified with D101 resin and the main composition was determined as chlorogenic acid, rutin, benzoic acid and hyperoside. MLPs demonstrated a strong inhibitory effect on disaccharidases derived from both mouse and Caco-2 cells, and the order of IC50 value was: murine sucrase (7.065 mg mL-1) > murine maltase (4.037 mg mL-1) > Caco-2 cell maltase (0.732 mg mL-1) > Caco-2 cell sucrase (0.146 mg mL-1). MLPs showed the strongest inhibitory effect on sucrase derived from Caco-2 cells and played a role in lowering postprandial glucose mainly by inhibiting sucrase activity. The Caco-2 monolayer cell model was established to simulate the glucose transport process in the human small intestine. We found that within the concentration range of 0.5-2 mg mL-1, MLPs significantly inhibited glucose transport, and the inhibition rate increased with time and dose. The effect of phlorizin (SGLT1 inhibitor) in the control group showed a similar effect on glucose transport, revealing that MLPs may inhibit glucose transport mainly by inhibiting the SGLT1 transporter. RT-qPCR analysis confirmed that MLPs inhibited glucose absorption by suppressing the SGLT1-GLUT2 pathway via downregulation of the mRNA expression of phospholipase, protein kinase A and protein kinase C.

Authors+Show Affiliations

Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research Institute /Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China. yuxiaozou@163.com liaost@163.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32064488

Citation

Li, Qian, et al. "Mulberry Leaf Polyphenols Attenuated Postprandial Glucose Absorption Via Inhibition of Disaccharidases Activity and Glucose Transport in Caco-2 Cells." Food & Function, vol. 11, no. 2, 2020, pp. 1835-1844.
Li Q, Wang C, Liu F, et al. Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in Caco-2 cells. Food Funct. 2020;11(2):1835-1844.
Li, Q., Wang, C., Liu, F., Hu, T., Shen, W., Li, E., Liao, S., & Zou, Y. (2020). Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in Caco-2 cells. Food & Function, 11(2), 1835-1844. https://doi.org/10.1039/c9fo01345h
Li Q, et al. Mulberry Leaf Polyphenols Attenuated Postprandial Glucose Absorption Via Inhibition of Disaccharidases Activity and Glucose Transport in Caco-2 Cells. Food Funct. 2020 Feb 26;11(2):1835-1844. PubMed PMID: 32064488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in Caco-2 cells. AU - Li,Qian, AU - Wang,Chen, AU - Liu,Fan, AU - Hu,Tenggen, AU - Shen,Weizhi, AU - Li,Erna, AU - Liao,Sentai, AU - Zou,Yuxiao, PY - 2020/2/18/pubmed PY - 2020/2/18/medline PY - 2020/2/18/entrez SP - 1835 EP - 1844 JF - Food & function JO - Food Funct VL - 11 IS - 2 N2 - The present study attempted to evaluate the mechanism of action and bioactivity of mulberry leaf polyphenols (MLPs) in type-2 diabetes prevention via inhibition of disaccharidase and glucose transport. MLPs were purified with D101 resin and the main composition was determined as chlorogenic acid, rutin, benzoic acid and hyperoside. MLPs demonstrated a strong inhibitory effect on disaccharidases derived from both mouse and Caco-2 cells, and the order of IC50 value was: murine sucrase (7.065 mg mL-1) > murine maltase (4.037 mg mL-1) > Caco-2 cell maltase (0.732 mg mL-1) > Caco-2 cell sucrase (0.146 mg mL-1). MLPs showed the strongest inhibitory effect on sucrase derived from Caco-2 cells and played a role in lowering postprandial glucose mainly by inhibiting sucrase activity. The Caco-2 monolayer cell model was established to simulate the glucose transport process in the human small intestine. We found that within the concentration range of 0.5-2 mg mL-1, MLPs significantly inhibited glucose transport, and the inhibition rate increased with time and dose. The effect of phlorizin (SGLT1 inhibitor) in the control group showed a similar effect on glucose transport, revealing that MLPs may inhibit glucose transport mainly by inhibiting the SGLT1 transporter. RT-qPCR analysis confirmed that MLPs inhibited glucose absorption by suppressing the SGLT1-GLUT2 pathway via downregulation of the mRNA expression of phospholipase, protein kinase A and protein kinase C. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/32064488/Mulberry_leaf_polyphenols_attenuated_postprandial_glucose_absorption_via_inhibition_of_disaccharidases_activity_and_glucose_transport_in_Caco-2_cells L2 - https://doi.org/10.1039/c9fo01345h DB - PRIME DP - Unbound Medicine ER -
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