Tags

Type your tag names separated by a space and hit enter

Quantification of Furanylfentanyl and its Metabolites in Human and Rat Plasma Using LC-MS-MS.
J Anal Toxicol. 2020 Jul 31; 44(6):589-595.JA

Abstract

Fentanyl analogs (novel and traditional) continue to impact the ever-growing opioid epidemic. Furanylfentanyl (FuF) is one analog equipotent to fentanyl that has documented involvement in thousands of intoxication and fatality cases around the world. Due to its prevalence, toxicologists need to improve detection and understanding of this analog. A method for the quantification of FuF and its metabolites (4-ANPP, furanyl norfentanyl (FuNorF)) in a small volume (100 μL) of human plasma by LC-MS-MS was developed and validated according to ANSI/ASB Standard. The method was cross validated in rat plasma for a future pharmacokinetic (PK)/pharmacodynamic (PD) study. In human plasma, calibration ranges were 0.025-25 ng/mL (FuF and 4-ANPP) and 0.5-25 ng/mL (FuNorF). Limits of detection were 0.0125 ng/mL (FuF and 4-ANPP) and 0.25 ng/mL (FuNorF). Lower limits of quantification coincided with lowest calibrator concentrations of 0.025 ng/mL (FuF and 4-ANPP) and 0.5 ng/mL (FuNorF). Precision and bias values were determined to be acceptable for all analytes. Matrix effects were acceptable for all analytes (-8.6-25.0%), except FuNorF with suppression >25%. Extraction recoveries ranged from 84.5 to 98.1%. No carryover or endogenous interferences were observed. Qualitative interferences with 4-ANPP were observed from some n-acyl substituted fentanyl analogs predicted to be low-concentration standard impurities. Analytes were stable under all conditions and dilution integrity was sustained. The method was successfully cross validated in rat plasma with acceptable bias (-7.4-8.4%), precision (within-run < 19%CV and between-run < 12.6%CV), matrix effects (-9.3-17.2%, except FuNorF with >25% suppression), recoveries (79.2-94.5%) and dilution integrity (1/2 and 1/10).

Authors+Show Affiliations

Department of Forensic Science, Sam Houston State University, 1905 University Ave, Huntsville, TX 77340, USA.Department of Forensic Science, Sam Houston State University, 1905 University Ave, Huntsville, TX 77340, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32064536

Citation

Palmquist, Kaitlyn B., and Madeleine J. Swortwood. "Quantification of Furanylfentanyl and Its Metabolites in Human and Rat Plasma Using LC-MS-MS." Journal of Analytical Toxicology, vol. 44, no. 6, 2020, pp. 589-595.
Palmquist KB, Swortwood MJ. Quantification of Furanylfentanyl and its Metabolites in Human and Rat Plasma Using LC-MS-MS. J Anal Toxicol. 2020;44(6):589-595.
Palmquist, K. B., & Swortwood, M. J. (2020). Quantification of Furanylfentanyl and its Metabolites in Human and Rat Plasma Using LC-MS-MS. Journal of Analytical Toxicology, 44(6), 589-595. https://doi.org/10.1093/jat/bkaa013
Palmquist KB, Swortwood MJ. Quantification of Furanylfentanyl and Its Metabolites in Human and Rat Plasma Using LC-MS-MS. J Anal Toxicol. 2020 Jul 31;44(6):589-595. PubMed PMID: 32064536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of Furanylfentanyl and its Metabolites in Human and Rat Plasma Using LC-MS-MS. AU - Palmquist,Kaitlyn B, AU - Swortwood,Madeleine J, PY - 2020/2/18/pubmed PY - 2020/9/24/medline PY - 2020/2/18/entrez SP - 589 EP - 595 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 44 IS - 6 N2 - Fentanyl analogs (novel and traditional) continue to impact the ever-growing opioid epidemic. Furanylfentanyl (FuF) is one analog equipotent to fentanyl that has documented involvement in thousands of intoxication and fatality cases around the world. Due to its prevalence, toxicologists need to improve detection and understanding of this analog. A method for the quantification of FuF and its metabolites (4-ANPP, furanyl norfentanyl (FuNorF)) in a small volume (100 μL) of human plasma by LC-MS-MS was developed and validated according to ANSI/ASB Standard. The method was cross validated in rat plasma for a future pharmacokinetic (PK)/pharmacodynamic (PD) study. In human plasma, calibration ranges were 0.025-25 ng/mL (FuF and 4-ANPP) and 0.5-25 ng/mL (FuNorF). Limits of detection were 0.0125 ng/mL (FuF and 4-ANPP) and 0.25 ng/mL (FuNorF). Lower limits of quantification coincided with lowest calibrator concentrations of 0.025 ng/mL (FuF and 4-ANPP) and 0.5 ng/mL (FuNorF). Precision and bias values were determined to be acceptable for all analytes. Matrix effects were acceptable for all analytes (-8.6-25.0%), except FuNorF with suppression >25%. Extraction recoveries ranged from 84.5 to 98.1%. No carryover or endogenous interferences were observed. Qualitative interferences with 4-ANPP were observed from some n-acyl substituted fentanyl analogs predicted to be low-concentration standard impurities. Analytes were stable under all conditions and dilution integrity was sustained. The method was successfully cross validated in rat plasma with acceptable bias (-7.4-8.4%), precision (within-run < 19%CV and between-run < 12.6%CV), matrix effects (-9.3-17.2%, except FuNorF with >25% suppression), recoveries (79.2-94.5%) and dilution integrity (1/2 and 1/10). SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/32064536/Quantification_of_Furanylfentanyl_and_its_Metabolites_in_Human_and_Rat_Plasma_Using_LC_MS_MS_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkaa013 DB - PRIME DP - Unbound Medicine ER -