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Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates.
J Med Microbiol. 2020 Mar; 69(3):436-442.JM

Abstract

Introduction.

Malassezia folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China.

Aim.

To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates.Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively.

Results.

Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were <1 μg ml-1. M. furfur was more susceptible to itraconazole, terbinafine and bifonazole but tolerant to miconazole compared with M. globosa (P<0.05). Synergistic effects between terbinafine and itraconazole or between tacrolimus and itraconazole, ketoconazole or terbinafine occurred in 6, 7, 6 and 9 out of 37 strains, respectively. Phosphatases, lipases and proteases were mainly secreted in 51 isolates.

Conclusions.

Itraconazole, posaconazole, voriconazole and ketoconazole are theagents against which there is greatest susceptibility. Synergistic effects between terbinafine and itraconazole or tacrolimas and antifungals may be irrelevant to clinical application. Overproduction of lipases could enhance the skin inhabitation of M. furfur.

Authors+Show Affiliations

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32068525

Citation

Li, Wei, et al. "Molecular Epidemiology, in Vitro Susceptibility and Exoenzyme Screening of Malassezia Clinical Isolates." Journal of Medical Microbiology, vol. 69, no. 3, 2020, pp. 436-442.
Li W, Zhang ZW, Luo Y, et al. Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates. J Med Microbiol. 2020;69(3):436-442.
Li, W., Zhang, Z. W., Luo, Y., Liang, N., Pi, X. X., & Fan, Y. M. (2020). Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates. Journal of Medical Microbiology, 69(3), 436-442. https://doi.org/10.1099/jmm.0.001161
Li W, et al. Molecular Epidemiology, in Vitro Susceptibility and Exoenzyme Screening of Malassezia Clinical Isolates. J Med Microbiol. 2020;69(3):436-442. PubMed PMID: 32068525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology, in vitro susceptibility and exoenzyme screening of Malassezia clinical isolates. AU - Li,Wei, AU - Zhang,Zi-Wei, AU - Luo,Yun, AU - Liang,Ni, AU - Pi,Xiao-Xue, AU - Fan,Yi-Ming, PY - 2020/2/19/pubmed PY - 2020/4/14/medline PY - 2020/2/19/entrez KW - Malassezia KW - antifungals KW - epidemiology KW - extracellular enzyme KW - folliculitis KW - pityriasis versicolor KW - tacrolimus SP - 436 EP - 442 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 69 IS - 3 N2 - Introduction. Malassezia folliculitis (MF) and pityriasis versicolor (PV) are common dermatoses caused by Malassezia species. Their molecular epidemiology, drug susceptibility and exoenzymes are rarely reported in China.Aim. To investigate the molecular epidemiology, drug susceptibility and enzymatic profile of Malassezia clinical isolates.Methodology. Malassezia strains were recovered from MF and PV patients and healthy subjects (HS) and identified by sequencing analysis. The minimum inhibitory concentrations (MICs) of nine antifungals (posaconazole, voriconazole, itraconazole, fluconazole, ketoconazole, miconazole, bifonazole, terbinafine and caspofungin) and tacrolimus, the interactions between three antifungals (itraconazole, ketoconazole and terbinafine) and tacrolimus, and the extracellular enzyme profile were evaluated using broth and checkerboard microdilution and the Api-Zym system, respectively.Results. Among 392 Malassezia isolates from 729 subjects (289 MF, 218 PV and 222 HS), Malassezia furfur and Malassezia globosa accounted for 67.86 and 18.88 %, respectively. M. furfur was the major species in MF and PV patients and HS. Among 60M. furfur and 50M. globosa strains, the MICs for itraconazole, posaconazole, voriconazole and ketoconazole were <1 μg ml-1. M. furfur was more susceptible to itraconazole, terbinafine and bifonazole but tolerant to miconazole compared with M. globosa (P<0.05). Synergistic effects between terbinafine and itraconazole or between tacrolimus and itraconazole, ketoconazole or terbinafine occurred in 6, 7, 6 and 9 out of 37 strains, respectively. Phosphatases, lipases and proteases were mainly secreted in 51 isolates.Conclusions. Itraconazole, posaconazole, voriconazole and ketoconazole are theagents against which there is greatest susceptibility. Synergistic effects between terbinafine and itraconazole or tacrolimas and antifungals may be irrelevant to clinical application. Overproduction of lipases could enhance the skin inhabitation of M. furfur. SN - 1473-5644 UR - https://www.unboundmedicine.com/medline/citation/32068525/Molecular_epidemiology_in_vitro_susceptibility_and_exoenzyme_screening_of_Malassezia_clinical_isolates_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.001161 DB - PRIME DP - Unbound Medicine ER -