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LC-QToF-MS method for quantification of ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma and its application.
Biomed Chromatogr. 2020 May; 34(5):e4812.BC

Abstract

In this research, we developed and validated a liquid chromatography coupled to mass spectrometry (LC-QToF-MS) method for simultaneous quantification of the anti-tuberculosis drugs ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma. Plasma samples spiked with cimetidine (internal standard) were extracted using protein precipitation with acetonitrile containing 1% formic acid. Separation was performed using a C18 column under flow gradient conditions with water and acetonitrile, both containing 5 mm ammonium formate and 0.1% formic acid. The method was validated according to the ANVISA and US Food and Drug Administration guidelines for bioanalytical method validation. The calibration curve was linear over a concentration range of 0.2-5 μg ml-1 for ethambutol, 0.2-7.5 μg ml-1 for isoniazid, 1-40 μg ml-1 for pyrazinamide and 0.25-2 μg ml-1 for rifampicin, all with adequate precision and accuracy. The method was reproducible, selective and free of carryover and matrix effects. The validated LC-QToF-MS method was successfully applied to real samples and shown to be applicable to future therapeutic and pharmacokinetic monitoring studies.

Authors+Show Affiliations

Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.Department of Internal Medicine, Federal University of Paraná, Curitiba, PR, Brazil. Doctor Heitor Vieira Dourado Tropical Medicine Foundation, Programa de Pós-Graduação em Medicinal Tropical, State University of Amazonas, Manaus, AM, Brazil.Academic Department of Chemistry and Biology, Federal University Technology - Paraná, Curitiba, PR, Brazil.Doctor Heitor Vieira Dourado Tropical Medicine Foundation, Programa de Pós-Graduação em Medicinal Tropical, State University of Amazonas, Manaus, AM, Brazil.Department of Pharmacy, Federal University of Paraná, Curitiba, PR, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32068899

Citation

Fachi, Mariana Millan, et al. "LC-QToF-MS Method for Quantification of Ethambutol, Isoniazid, Pyrazinamide and Rifampicin in Human Plasma and Its Application." Biomedical Chromatography : BMC, vol. 34, no. 5, 2020, pp. e4812.
Fachi MM, Vilhena RO, Boger B, et al. LC-QToF-MS method for quantification of ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma and its application. Biomed Chromatogr. 2020;34(5):e4812.
Fachi, M. M., Vilhena, R. O., Boger, B., Domingos, E. L., Dos Santos, J. M. M. F., Junkert, A. M., de Fátima Cobre, A., Momade, D. R. O., Beraldi-Magalhães, F., de Liz, M. V., Cordeiro-Santos, M., & Pontarolo, R. (2020). LC-QToF-MS method for quantification of ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma and its application. Biomedical Chromatography : BMC, 34(5), e4812. https://doi.org/10.1002/bmc.4812
Fachi MM, et al. LC-QToF-MS Method for Quantification of Ethambutol, Isoniazid, Pyrazinamide and Rifampicin in Human Plasma and Its Application. Biomed Chromatogr. 2020;34(5):e4812. PubMed PMID: 32068899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LC-QToF-MS method for quantification of ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma and its application. AU - Fachi,Mariana Millan, AU - Vilhena,Raquel Oliveira, AU - Boger,Beatriz, AU - Domingos,Eric Luiz, AU - Dos Santos,Josiane Marlei Müller Fernandes, AU - Junkert,Allan Michael, AU - de Fátima Cobre,Alexandre, AU - Momade,Danilo Raul Ossufo, AU - Beraldi-Magalhães,Francisco, AU - de Liz,Marcus Vinicius, AU - Cordeiro-Santos,Marcelo, AU - Pontarolo,Roberto, Y1 - 2020/03/02/ PY - 2019/11/26/received PY - 2020/02/04/revised PY - 2020/02/14/accepted PY - 2020/2/19/pubmed PY - 2020/2/19/medline PY - 2020/2/19/entrez KW - LC-QToF-MS KW - anti-tuberculosis drugs KW - human plasma KW - validation SP - e4812 EP - e4812 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 34 IS - 5 N2 - In this research, we developed and validated a liquid chromatography coupled to mass spectrometry (LC-QToF-MS) method for simultaneous quantification of the anti-tuberculosis drugs ethambutol, isoniazid, pyrazinamide and rifampicin in human plasma. Plasma samples spiked with cimetidine (internal standard) were extracted using protein precipitation with acetonitrile containing 1% formic acid. Separation was performed using a C18 column under flow gradient conditions with water and acetonitrile, both containing 5 mm ammonium formate and 0.1% formic acid. The method was validated according to the ANVISA and US Food and Drug Administration guidelines for bioanalytical method validation. The calibration curve was linear over a concentration range of 0.2-5 μg ml-1 for ethambutol, 0.2-7.5 μg ml-1 for isoniazid, 1-40 μg ml-1 for pyrazinamide and 0.25-2 μg ml-1 for rifampicin, all with adequate precision and accuracy. The method was reproducible, selective and free of carryover and matrix effects. The validated LC-QToF-MS method was successfully applied to real samples and shown to be applicable to future therapeutic and pharmacokinetic monitoring studies. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/32068899/LC-QToF-MS_method_for_quantification_of_ethambutol,_isoniazid,_pyrazinamide_and_rifampicin_in_human_plasma_and_its_application L2 - https://doi.org/10.1002/bmc.4812 DB - PRIME DP - Unbound Medicine ER -
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