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Development, identification, and characterization of blue-grained wheat-Triticum boeoticum substitution lines.
J Appl Genet. 2020 May; 61(2):169-177.JA

Abstract

Diploid wild einkorn wheat, Triticum boeoticum Boiss (AbAb, 2n = 2x = 14), is a wheat-related species with a blue aleurone layer. In this study, six blue-grained wheat lines were developed from F8 progeny of crosses between common wheat and T. boeoticum. The chromosome constitutions of these lines were characterized by fluorescence in situ hybridization (FISH) using the oligonucleotide probes Oligo-pTa535-1, Oligo-pSc119.2-1, Oligo-pTa71-2, and (AAC)7. Multicolor FISH using Oligo-pTa535-1, Oligo-pSc119.2-1, and Oligo-pTa71-2 identified all 42 common wheat chromosomes, while Oligo-pTa535-1 and (AAC)7 discriminated the 14 chromosomes of T. boeoticum. FISH revealed that all six blue-grained lines were wheat-T. boeoticum 4Ab (4B) disomic substitution lines. The substitution lines were validated by genotyping using the wheat 55 K single nucleotide polymorphism (SNP) array containing 53,063 markers. These 4Ab (4B) substitution lines represent novel germplasm for blue-grained wheat breeding. The FISH probes and SNP markers used here can be applied in the development of blue-grained wheat-Triticum boeoticum translocation lines.

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Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. zhanglianquan1977@126.com. Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, China. zhanglianquan1977@126.com.

MeSH

BreedingChromosomes, PlantCrosses, GeneticDiploidyEdible GrainIn Situ Hybridization, FluorescenceOligonucleotidesPlant ProteinsPolymorphism, Single NucleotideTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32072449

Citation

Liu, Xin, et al. "Development, Identification, and Characterization of Blue-grained wheat-Triticum Boeoticum Substitution Lines." Journal of Applied Genetics, vol. 61, no. 2, 2020, pp. 169-177.

Liu X, Feng Z, Liang D, et al. Development, identification, and characterization of blue-grained wheat-Triticum boeoticum substitution lines. J Appl Genet. 2020;61(2):169-177.

Liu, X., Feng, Z., Liang, D., Zhang, M., Liu, X., Hao, M., Liu, D., Ning, S., Yuan, Z., Jiang, B., Chen, X., Chen, X., & Zhang, L. (2020). Development, identification, and characterization of blue-grained wheat-Triticum boeoticum substitution lines. Journal of Applied Genetics, 61(2), 169-177. https://doi.org/10.1007/s13353-020-00553-9

Liu X, et al. Development, Identification, and Characterization of Blue-grained wheat-Triticum Boeoticum Substitution Lines. J Appl Genet. 2020;61(2):169-177. PubMed PMID: 32072449.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development, identification, and characterization of blue-grained wheat-Triticum boeoticum substitution lines. AU - Liu,Xin, AU - Feng,Zhen, AU - Liang,Dongyu, AU - Zhang,Minghu, AU - Liu,Xiaojuan, AU - Hao,Ming, AU - Liu,Dengcai, AU - Ning,Shunzong, AU - Yuan,Zhongwei, AU - Jiang,Bo, AU - Chen,Xuejiao, AU - Chen,Xue, AU - Zhang,Lianquan, Y1 - 2020/02/18/ PY - 2019/11/13/received PY - 2020/02/12/accepted PY - 2020/02/06/revised PY - 2020/2/20/pubmed PY - 2020/12/29/medline PY - 2020/2/20/entrez KW - Blue aleurone KW - Fluorescence in situ hybridization KW - Single nucleotide polymorphism KW - Substitution line KW - Triticum boeoticum SP - 169 EP - 177 JF - Journal of applied genetics JO - J Appl Genet VL - 61 IS - 2 N2 - Diploid wild einkorn wheat, Triticum boeoticum Boiss (AbAb, 2n = 2x = 14), is a wheat-related species with a blue aleurone layer. In this study, six blue-grained wheat lines were developed from F8 progeny of crosses between common wheat and T. boeoticum. The chromosome constitutions of these lines were characterized by fluorescence in situ hybridization (FISH) using the oligonucleotide probes Oligo-pTa535-1, Oligo-pSc119.2-1, Oligo-pTa71-2, and (AAC)7. Multicolor FISH using Oligo-pTa535-1, Oligo-pSc119.2-1, and Oligo-pTa71-2 identified all 42 common wheat chromosomes, while Oligo-pTa535-1 and (AAC)7 discriminated the 14 chromosomes of T. boeoticum. FISH revealed that all six blue-grained lines were wheat-T. boeoticum 4Ab (4B) disomic substitution lines. The substitution lines were validated by genotyping using the wheat 55 K single nucleotide polymorphism (SNP) array containing 53,063 markers. These 4Ab (4B) substitution lines represent novel germplasm for blue-grained wheat breeding. The FISH probes and SNP markers used here can be applied in the development of blue-grained wheat-Triticum boeoticum translocation lines. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/32072449/Development_identification_and_characterization_of_blue_grained_wheat_Triticum_boeoticum_substitution_lines_ L2 - https://dx.doi.org/10.1007/s13353-020-00553-9 DB - PRIME DP - Unbound Medicine ER -