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Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes.
Artif Cells Nanomed Biotechnol. 2020 Dec; 48(1):672-682.AC

Abstract

The present study highlights the biological synthesis of silver nanoparticles (AgNPs) using Sphingobium sp. MAH-11 and also their antibacterial mechanisms against drug-resistant pathogenic microorganisms. The nanoparticle synthesis method used in this study was reliable, facile, rapid, cost-effective and ecofriendly. The AgNPs exhibited the highest absorbance at 423 nm. The TEM image expressed spherical shape of AgNPs and the size of synthesized AgNPs was 7-22 nm. The selected area diffraction (SAED) pattern and XRD spectrum revealed the crystalline structure of AgNPs. The results of FTIR analysis disclosed the functional groups responsible for the reduction of silver ion to metal nanoparticles. The biosynthesized AgNPs showed strong anti-microbial activity against drug-resistant pathogenic microorganisms. Moreover, Escherichia coli and Staphylococcus aureus were used to explore the antibacterial mechanisms of biosynthesized AgNPs. Minimal inhibitory concentrations (MICs) of E. coli and S. aureus were 6.25 μg/mL and 50 μg/mL, respectively and minimum bactericidal concentrations (MBCs) of E. coli and S. aureus were 25 μg/mL and 100 μg/mL, respectively. Results exhibited that biosynthesized AgNPs caused morphological changes and injured the membrane integrity of strains E. coli and S. aureus. The AgNPs synthesized by Sphingobium sp. MAH-11 may serve as a potent antimicrobial agent for many therapeutic applications.

Authors+Show Affiliations

Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, Republic of Korea.Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32075448

Citation

Akter, Shahina, and Md Amdadul Huq. "Biologically Rapid Synthesis of Silver Nanoparticles By Sphingobium Sp. MAH-11T and Their Antibacterial Activity and Mechanisms Investigation Against Drug-resistant Pathogenic Microbes." Artificial Cells, Nanomedicine, and Biotechnology, vol. 48, no. 1, 2020, pp. 672-682.
Akter S, Huq MA. Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes. Artif Cells Nanomed Biotechnol. 2020;48(1):672-682.
Akter, S., & Huq, M. A. (2020). Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes. Artificial Cells, Nanomedicine, and Biotechnology, 48(1), 672-682. https://doi.org/10.1080/21691401.2020.1730390
Akter S, Huq MA. Biologically Rapid Synthesis of Silver Nanoparticles By Sphingobium Sp. MAH-11T and Their Antibacterial Activity and Mechanisms Investigation Against Drug-resistant Pathogenic Microbes. Artif Cells Nanomed Biotechnol. 2020;48(1):672-682. PubMed PMID: 32075448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biologically rapid synthesis of silver nanoparticles by Sphingobium sp. MAH-11T and their antibacterial activity and mechanisms investigation against drug-resistant pathogenic microbes. AU - Akter,Shahina, AU - Huq,Md Amdadul, PY - 2020/2/21/entrez PY - 2020/2/23/pubmed PY - 2020/11/12/medline KW - AgNPs KW - Biological synthesis KW - Sphingobium sp. MAH-11 KW - antimicrobial agent SP - 672 EP - 682 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol VL - 48 IS - 1 N2 - The present study highlights the biological synthesis of silver nanoparticles (AgNPs) using Sphingobium sp. MAH-11 and also their antibacterial mechanisms against drug-resistant pathogenic microorganisms. The nanoparticle synthesis method used in this study was reliable, facile, rapid, cost-effective and ecofriendly. The AgNPs exhibited the highest absorbance at 423 nm. The TEM image expressed spherical shape of AgNPs and the size of synthesized AgNPs was 7-22 nm. The selected area diffraction (SAED) pattern and XRD spectrum revealed the crystalline structure of AgNPs. The results of FTIR analysis disclosed the functional groups responsible for the reduction of silver ion to metal nanoparticles. The biosynthesized AgNPs showed strong anti-microbial activity against drug-resistant pathogenic microorganisms. Moreover, Escherichia coli and Staphylococcus aureus were used to explore the antibacterial mechanisms of biosynthesized AgNPs. Minimal inhibitory concentrations (MICs) of E. coli and S. aureus were 6.25 μg/mL and 50 μg/mL, respectively and minimum bactericidal concentrations (MBCs) of E. coli and S. aureus were 25 μg/mL and 100 μg/mL, respectively. Results exhibited that biosynthesized AgNPs caused morphological changes and injured the membrane integrity of strains E. coli and S. aureus. The AgNPs synthesized by Sphingobium sp. MAH-11 may serve as a potent antimicrobial agent for many therapeutic applications. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/32075448/Biologically_rapid_synthesis_of_silver_nanoparticles_by_Sphingobium_sp__MAH_11T_and_their_antibacterial_activity_and_mechanisms_investigation_against_drug_resistant_pathogenic_microbes_ L2 - https://www.tandfonline.com/doi/full/10.1080/21691401.2020.1730390 DB - PRIME DP - Unbound Medicine ER -