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The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase.
Biochem Biophys Res Commun. 2020 Feb 18 [Online ahead of print]BB

Abstract

GPI anchored proteins (GPI-APs) act at the frontiers of cells, decoding environmental cues and determining host-pathogen interactions in several lower eukaryotes. They are also essential for viability in lower eukaryotes. The GPI biosynthetic pathway begins at the ER and follows a roughly linear pathway to generate the complete precursor (CP) glycolipid. The GPI transamidase (GPIT) transfers this glycolipid to the C-terminal end of newly translated proteins after removing their GPI attachment signal sequence (SS). The GPIT subunit that cleaves SS is Gpi8, a protein with a conserved Cys/His catalytic dyad typical of cysteine proteases. A CaGPI8 heterozygous mutant accumulates CPs and has reduced cell surface GPI-APs. Using a simple cell-free assay, we demonstrate that the heterozygous CaGPI8 strain has low endopeptidase activity as well. The revertant strain is restored in all these phenotypes. CaGpi8 is also shown to be a metalloenzyme, whose protease activity is sensitive to agents that modify Cys/His residues.

Authors+Show Affiliations

School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: sskomath@mail.jnu.ac.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32081427

Citation

Sah, Sudisht Kumar, et al. "The Caspase-like Gpi8 Subunit of Candida Albicans GPI Transamidase Is a Metal-dependent Endopeptidase." Biochemical and Biophysical Research Communications, 2020.
Sah SK, Shefali S, Yadav A, et al. The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase. Biochem Biophys Res Commun. 2020.
Sah, S. K., Shefali, S., Yadav, A., Som, P., & Komath, S. S. (2020). The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase. Biochemical and Biophysical Research Communications. https://doi.org/10.1016/j.bbrc.2020.02.008
Sah SK, et al. The Caspase-like Gpi8 Subunit of Candida Albicans GPI Transamidase Is a Metal-dependent Endopeptidase. Biochem Biophys Res Commun. 2020 Feb 18; PubMed PMID: 32081427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The caspase-like Gpi8 subunit of Candida albicans GPI transamidase is a metal-dependent endopeptidase. AU - Sah,Sudisht Kumar, AU - Shefali,Shailja, AU - Yadav,Anshuman, AU - Som,Punnag, AU - Komath,Sneha Sudha, Y1 - 2020/02/18/ PY - 2020/01/22/received PY - 2020/02/03/accepted PY - 2020/2/23/pubmed PY - 2020/2/23/medline PY - 2020/2/22/entrez KW - Cell-free assay KW - Endopeptidase KW - GPI transamidase KW - Metabolic labelling KW - Metalloenzyme JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. N2 - GPI anchored proteins (GPI-APs) act at the frontiers of cells, decoding environmental cues and determining host-pathogen interactions in several lower eukaryotes. They are also essential for viability in lower eukaryotes. The GPI biosynthetic pathway begins at the ER and follows a roughly linear pathway to generate the complete precursor (CP) glycolipid. The GPI transamidase (GPIT) transfers this glycolipid to the C-terminal end of newly translated proteins after removing their GPI attachment signal sequence (SS). The GPIT subunit that cleaves SS is Gpi8, a protein with a conserved Cys/His catalytic dyad typical of cysteine proteases. A CaGPI8 heterozygous mutant accumulates CPs and has reduced cell surface GPI-APs. Using a simple cell-free assay, we demonstrate that the heterozygous CaGPI8 strain has low endopeptidase activity as well. The revertant strain is restored in all these phenotypes. CaGpi8 is also shown to be a metalloenzyme, whose protease activity is sensitive to agents that modify Cys/His residues. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/32081427/The_caspase-like_Gpi8_subunit_of_Candida_albicans_GPI_transamidase_is_a_metal-dependent_endopeptidase L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(20)30268-0 DB - PRIME DP - Unbound Medicine ER -
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