Tags

Type your tag names separated by a space and hit enter

Elucidating Toxicodynamic Differences at the Molecular Scale between ZnO Nanoparticles and ZnCl2 in Enchytraeus crypticus via Nontargeted Metabolomics.
Environ Sci Technol. 2020 03 17; 54(6):3487-3498.ES

Abstract

Much effort has been devoted to clarifying the comparative toxicity of ZnO nanoparticles (NPs) and Zn ions; however, little is known about their toxicodynamic processes at the metabolic level. Here, we investigated the acute (2d) and chronic (7d) effects to a soil species, Enchytraeus crypticus, of two sublethal doses of ZnO-NPs and ZnCl2 (10 and 30 mg/L Zn) using ultrahigh performance liquid chromatography-quadrupole-time-of-flight/mass spectrometry-based metabolomics. The metabolomics analysis identified 99, 128, 121, and 183 significantly changed metabolites (SCMs) in E. crypticus exposed to ZnO-NPs for 2d, ZnCl2 for 2d, ZnO-NPs for 7d, and ZnCl2 for 7d, respectively, suggesting that ZnCl2 induced stronger metabolic reprogramming than ZnO-NPs, and a longer exposure time caused greater metabolite changes. Among the SCMs, 67 were shared by ZnO-NPs and ZnCl2 after 2d and 84 after 7d. These metabolites were mainly related to oxidative stress and antioxidant defense, membrane disturbance, and energy expenditure. The targeted analysis on physiological and biochemical responses further proved the metabolic observations. Nevertheless, 32 (33%) and 37 (31%) SCMs were found only in ZnO-NP treatments after 2 and 7d, respectively, suggesting that the toxicity of ZnO-NPs cannot be solely attributed to the released Zn ions. Metabolic pathway analysis revealed significant perturbations of galactose metabolism, amino sugar and nucleotide sugar metabolism, and glycerophospholipid metabolism in all test groups. Based on involvement frequency, glucose-1-phosphate, glycerol 3-phosphate, and phosphorylcholine could serve as universal biomarkers for exposure to different Zn forms. Four pathways perturbed by ZnO-NPs were nanospecific upon acute exposure and three upon chronic exposure. Our findings demonstrated that metabolomics is an effective tool for understanding the molecular toxicity mechanism and highlighted that time-series measurements are essential for discovering and comparing modes of action of metal ions and NPs.

Authors+Show Affiliations

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China.National Institute of Public Health and the Environment, Center for the Safety of Substances and Products, Bilthoven 3720 BA, The Netherlands.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32083472

Citation

He, Erkai, et al. "Elucidating Toxicodynamic Differences at the Molecular Scale Between ZnO Nanoparticles and ZnCl2 in Enchytraeus Crypticus Via Nontargeted Metabolomics." Environmental Science & Technology, vol. 54, no. 6, 2020, pp. 3487-3498.
He E, Qiu R, Cao X, et al. Elucidating Toxicodynamic Differences at the Molecular Scale between ZnO Nanoparticles and ZnCl2 in Enchytraeus crypticus via Nontargeted Metabolomics. Environ Sci Technol. 2020;54(6):3487-3498.
He, E., Qiu, R., Cao, X., Song, L., Peijnenburg, W. J. G. M., & Qiu, H. (2020). Elucidating Toxicodynamic Differences at the Molecular Scale between ZnO Nanoparticles and ZnCl2 in Enchytraeus crypticus via Nontargeted Metabolomics. Environmental Science & Technology, 54(6), 3487-3498. https://doi.org/10.1021/acs.est.0c00663
He E, et al. Elucidating Toxicodynamic Differences at the Molecular Scale Between ZnO Nanoparticles and ZnCl2 in Enchytraeus Crypticus Via Nontargeted Metabolomics. Environ Sci Technol. 2020 03 17;54(6):3487-3498. PubMed PMID: 32083472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elucidating Toxicodynamic Differences at the Molecular Scale between ZnO Nanoparticles and ZnCl2 in Enchytraeus crypticus via Nontargeted Metabolomics. AU - He,Erkai, AU - Qiu,Rongliang, AU - Cao,Xinde, AU - Song,Lan, AU - Peijnenburg,Willie J G M, AU - Qiu,Hao, Y1 - 2020/03/02/ PY - 2020/2/23/pubmed PY - 2020/3/28/medline PY - 2020/2/22/entrez SP - 3487 EP - 3498 JF - Environmental science & technology JO - Environ Sci Technol VL - 54 IS - 6 N2 - Much effort has been devoted to clarifying the comparative toxicity of ZnO nanoparticles (NPs) and Zn ions; however, little is known about their toxicodynamic processes at the metabolic level. Here, we investigated the acute (2d) and chronic (7d) effects to a soil species, Enchytraeus crypticus, of two sublethal doses of ZnO-NPs and ZnCl2 (10 and 30 mg/L Zn) using ultrahigh performance liquid chromatography-quadrupole-time-of-flight/mass spectrometry-based metabolomics. The metabolomics analysis identified 99, 128, 121, and 183 significantly changed metabolites (SCMs) in E. crypticus exposed to ZnO-NPs for 2d, ZnCl2 for 2d, ZnO-NPs for 7d, and ZnCl2 for 7d, respectively, suggesting that ZnCl2 induced stronger metabolic reprogramming than ZnO-NPs, and a longer exposure time caused greater metabolite changes. Among the SCMs, 67 were shared by ZnO-NPs and ZnCl2 after 2d and 84 after 7d. These metabolites were mainly related to oxidative stress and antioxidant defense, membrane disturbance, and energy expenditure. The targeted analysis on physiological and biochemical responses further proved the metabolic observations. Nevertheless, 32 (33%) and 37 (31%) SCMs were found only in ZnO-NP treatments after 2 and 7d, respectively, suggesting that the toxicity of ZnO-NPs cannot be solely attributed to the released Zn ions. Metabolic pathway analysis revealed significant perturbations of galactose metabolism, amino sugar and nucleotide sugar metabolism, and glycerophospholipid metabolism in all test groups. Based on involvement frequency, glucose-1-phosphate, glycerol 3-phosphate, and phosphorylcholine could serve as universal biomarkers for exposure to different Zn forms. Four pathways perturbed by ZnO-NPs were nanospecific upon acute exposure and three upon chronic exposure. Our findings demonstrated that metabolomics is an effective tool for understanding the molecular toxicity mechanism and highlighted that time-series measurements are essential for discovering and comparing modes of action of metal ions and NPs. SN - 1520-5851 UR - https://www.unboundmedicine.com/medline/citation/32083472/Elucidating_Toxicodynamic_Differences_at_the_Molecular_Scale_between_ZnO_Nanoparticles_and_ZnCl2_in_Enchytraeus_crypticus_via_Nontargeted_Metabolomics_ L2 - https://doi.org/10.1021/acs.est.0c00663 DB - PRIME DP - Unbound Medicine ER -