Tags

Type your tag names separated by a space and hit enter

Evaluation of porcine diamine oxidase for the conversion of histamine in food-relevant amounts.
J Food Sci. 2020 Mar; 85(3):843-852.JF

Abstract

Histamine exists in a multitude of foods and displays an emerging role within food intolerances. Our aim was to identify the activity of porcine diamine oxidase (DAO) required for the in vitro degradation of histamine amounts that are found in typical meals containing histamine (75 mg, equaled 150 mg/L). Furthermore, we investigated an actual dietary supplement that is commercially available for histamine intolerant individuals for its histamine reduction capability. Kinetic investigations of porcine DAO showed a substrate inhibition by histamine concentrations greater than 56 mg/L (0.5 mM). The stability of free porcine DAO was tested in a fed state simulated intestinal fluid and exhibited a half-life period of around 19 min. A total of 50 nanokatal (nkat) free porcine DAO, which equaled the amount of enzyme isolated from around 100 g pig kidney, were necessary for the in vitro reduction of around 90% of the histamine. The dietary supplement that contains a pig kidney extract did not show DAO activity. Instead, the used histamine (0.75 mg) was apparently reduced due to the adsorption of histamine onto a capsule component by 18.9 ± 2.3% within 5 hr. Although the capsule preparation retained its overall structure and shape for at least 90 min in simulated gastric fluid, the apparent histamine reduction was significantly reduced to 12.1 ± 2.3% (P ≤ 0.05). In conclusion, an alternative to the pig kidney DAO or an improved capsule preparation is needed to ensure an adequate supplementation for histamine-intolerant humans. PRACTICAL APPLICATION: Histamine intolerance is an emerging issue in our society and the intolerance-related physiological symptoms are currently not reliably treatable due to a lack of scientific investigation. A commercially available dietary supplement for histamine intolerance does not fulfil the requirements for a satisfactory histamine reduction in intolerant humans. The activity of the histamine degrading enzyme diamine oxidase, required for a satisfactory histamine degradation, is by far higher than the theoretical amount apparently given in the dietary supplement. With this knowledge, it is obvious that improved food supplements must be developed to help histamine intolerant humans.

Authors+Show Affiliations

Dept. of Biotechnology and Enzyme Science, Inst. of Food Science and Biotechnology, Univ. of Hohenheim, Garbenstr. 25, 70599, Stuttgart, Germany.Dept. of Biotechnology and Enzyme Science, Inst. of Food Science and Biotechnology, Univ. of Hohenheim, Garbenstr. 25, 70599, Stuttgart, Germany.Dept. of Biotechnology and Enzyme Science, Inst. of Food Science and Biotechnology, Univ. of Hohenheim, Garbenstr. 25, 70599, Stuttgart, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32090335

Citation

Kettner, Lucas, et al. "Evaluation of Porcine Diamine Oxidase for the Conversion of Histamine in Food-relevant Amounts." Journal of Food Science, vol. 85, no. 3, 2020, pp. 843-852.
Kettner L, Seitl I, Fischer L. Evaluation of porcine diamine oxidase for the conversion of histamine in food-relevant amounts. J Food Sci. 2020;85(3):843-852.
Kettner, L., Seitl, I., & Fischer, L. (2020). Evaluation of porcine diamine oxidase for the conversion of histamine in food-relevant amounts. Journal of Food Science, 85(3), 843-852. https://doi.org/10.1111/1750-3841.15069
Kettner L, Seitl I, Fischer L. Evaluation of Porcine Diamine Oxidase for the Conversion of Histamine in Food-relevant Amounts. J Food Sci. 2020;85(3):843-852. PubMed PMID: 32090335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of porcine diamine oxidase for the conversion of histamine in food-relevant amounts. AU - Kettner,Lucas, AU - Seitl,Ines, AU - Fischer,Lutz, Y1 - 2020/02/23/ PY - 2019/09/20/received PY - 2020/01/10/revised PY - 2020/01/10/accepted PY - 2020/2/25/pubmed PY - 2020/7/1/medline PY - 2020/2/25/entrez KW - biogenic amine KW - diamine oxidase KW - dietary supplement KW - histamine KW - histamine intolerance SP - 843 EP - 852 JF - Journal of food science JO - J. Food Sci. VL - 85 IS - 3 N2 - Histamine exists in a multitude of foods and displays an emerging role within food intolerances. Our aim was to identify the activity of porcine diamine oxidase (DAO) required for the in vitro degradation of histamine amounts that are found in typical meals containing histamine (75 mg, equaled 150 mg/L). Furthermore, we investigated an actual dietary supplement that is commercially available for histamine intolerant individuals for its histamine reduction capability. Kinetic investigations of porcine DAO showed a substrate inhibition by histamine concentrations greater than 56 mg/L (0.5 mM). The stability of free porcine DAO was tested in a fed state simulated intestinal fluid and exhibited a half-life period of around 19 min. A total of 50 nanokatal (nkat) free porcine DAO, which equaled the amount of enzyme isolated from around 100 g pig kidney, were necessary for the in vitro reduction of around 90% of the histamine. The dietary supplement that contains a pig kidney extract did not show DAO activity. Instead, the used histamine (0.75 mg) was apparently reduced due to the adsorption of histamine onto a capsule component by 18.9 ± 2.3% within 5 hr. Although the capsule preparation retained its overall structure and shape for at least 90 min in simulated gastric fluid, the apparent histamine reduction was significantly reduced to 12.1 ± 2.3% (P ≤ 0.05). In conclusion, an alternative to the pig kidney DAO or an improved capsule preparation is needed to ensure an adequate supplementation for histamine-intolerant humans. PRACTICAL APPLICATION: Histamine intolerance is an emerging issue in our society and the intolerance-related physiological symptoms are currently not reliably treatable due to a lack of scientific investigation. A commercially available dietary supplement for histamine intolerance does not fulfil the requirements for a satisfactory histamine reduction in intolerant humans. The activity of the histamine degrading enzyme diamine oxidase, required for a satisfactory histamine degradation, is by far higher than the theoretical amount apparently given in the dietary supplement. With this knowledge, it is obvious that improved food supplements must be developed to help histamine intolerant humans. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/32090335/Evaluation_of_porcine_diamine_oxidase_for_the_conversion_of_histamine_in_food_relevant_amounts_ L2 - https://doi.org/10.1111/1750-3841.15069 DB - PRIME DP - Unbound Medicine ER -