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Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots.
Ecotoxicol Environ Saf. 2020 Apr 15; 193:110245.EE

Abstract

Understanding Cd uptake and distribution in rice roots is important for breeding varieties that do not accumulate Cd in the grain to any large extent. Here, we examined the physiological and molecular factors responsible for Cd uptake and transport differences between two japonica rice cultivars prescreened as high (zhefu7) or low (Xiangzaoxian45) accumulators of Cd in the grain. No significant differences in Cd uptake between the two cultivars were observed; however, Xiangzaoxian45 retained most of the absorbed Cd in the roots, whereas zhefu7 showed higher transport of Cd from the root to the shoot, regardless of the duration of exposure to Cd. The inability to sequester Cd into root vacuoles caused high accumulation of Cd in the grain in zhefu7, whereas inefficient transport of Cd from roots to shoots in Xiangzaoxian45 caused low accumulation of Cd in the grain. Cd sequestration in the roots and transport from the root to the shoot were greatly influenced by the expression patterns of transport-related genes OsHMA3 and OsHMA2, respectively. Further, micro-X-ray fluorescence spectroscopy mapping confirmed that more Cd was sequestered in the roots of Xiangzaoxian45 than in those of zhefu7, with a significant amount of Cd localized in the root hairs, as well as in the meristematic and elongation zones, and dermal and stele tissues. Therefore, we propose that effective Cd sequestration in root vacuoles was the major determinant of divergent Cd-accumulation patterns in the two rice cultivars under study.

Authors+Show Affiliations

Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China; Department of Plant Sciences, Salale University, Fitche, Ethiopia.Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China.Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China.Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China.Stanford Synchrotron Radiation Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA, 94025, USA.Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China; Department of Plant Sciences, Salale University, Fitche, Ethiopia.Key Laboratory of Environment Remediation and Ecological Health (Zhejiang University, College of Environmental & Resource Science), Ministry of Education, Hangzhou, 310058, China. Electronic address: tiansk@zju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32092577

Citation

Tefera, Wolde, et al. "Micro-XRF Mapping and Quantitative Assessment of Cd in Rice (Oryza Sativa L.) Roots." Ecotoxicology and Environmental Safety, vol. 193, 2020, p. 110245.
Tefera W, Liu T, Lu L, et al. Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots. Ecotoxicol Environ Saf. 2020;193:110245.
Tefera, W., Liu, T., Lu, L., Ge, J., Webb, S. M., Seifu, W., & Tian, S. (2020). Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots. Ecotoxicology and Environmental Safety, 193, 110245. https://doi.org/10.1016/j.ecoenv.2020.110245
Tefera W, et al. Micro-XRF Mapping and Quantitative Assessment of Cd in Rice (Oryza Sativa L.) Roots. Ecotoxicol Environ Saf. 2020 Apr 15;193:110245. PubMed PMID: 32092577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots. AU - Tefera,Wolde, AU - Liu,Ting, AU - Lu,Lingli, AU - Ge,Jun, AU - Webb,Samuel M, AU - Seifu,Weldemariam, AU - Tian,Shengke, Y1 - 2020/02/21/ PY - 2019/12/02/received PY - 2020/01/18/revised PY - 2020/01/22/accepted PY - 2020/2/25/pubmed PY - 2020/5/28/medline PY - 2020/2/25/entrez KW - Cd sequestration KW - Cd transporter gene KW - Micro-x-ray fluorescence KW - Rice KW - Root-to-shoot transport SP - 110245 EP - 110245 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 193 N2 - Understanding Cd uptake and distribution in rice roots is important for breeding varieties that do not accumulate Cd in the grain to any large extent. Here, we examined the physiological and molecular factors responsible for Cd uptake and transport differences between two japonica rice cultivars prescreened as high (zhefu7) or low (Xiangzaoxian45) accumulators of Cd in the grain. No significant differences in Cd uptake between the two cultivars were observed; however, Xiangzaoxian45 retained most of the absorbed Cd in the roots, whereas zhefu7 showed higher transport of Cd from the root to the shoot, regardless of the duration of exposure to Cd. The inability to sequester Cd into root vacuoles caused high accumulation of Cd in the grain in zhefu7, whereas inefficient transport of Cd from roots to shoots in Xiangzaoxian45 caused low accumulation of Cd in the grain. Cd sequestration in the roots and transport from the root to the shoot were greatly influenced by the expression patterns of transport-related genes OsHMA3 and OsHMA2, respectively. Further, micro-X-ray fluorescence spectroscopy mapping confirmed that more Cd was sequestered in the roots of Xiangzaoxian45 than in those of zhefu7, with a significant amount of Cd localized in the root hairs, as well as in the meristematic and elongation zones, and dermal and stele tissues. Therefore, we propose that effective Cd sequestration in root vacuoles was the major determinant of divergent Cd-accumulation patterns in the two rice cultivars under study. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/32092577/Micro_XRF_mapping_and_quantitative_assessment_of_Cd_in_rice__Oryza_sativa_L___roots_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(20)30084-1 DB - PRIME DP - Unbound Medicine ER -