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Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study.
Int J Mol Sci. 2020 Feb 20; 21(4)IJ

Abstract

Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer's recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.

Authors+Show Affiliations

Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32093379

Citation

Martin, Lynn M., et al. "Effects of Orally Administered Resveratrol On TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: a Prospective, Randomized, Double-Blinded, Placebo-Controlled Study." International Journal of Molecular Sciences, vol. 21, no. 4, 2020.
Martin LM, Johnson PJ, Amorim JR, et al. Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. Int J Mol Sci. 2020;21(4).
Martin, L. M., Johnson, P. J., Amorim, J. R., & DeClue, A. E. (2020). Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. International Journal of Molecular Sciences, 21(4). https://doi.org/10.3390/ijms21041453
Martin LM, et al. Effects of Orally Administered Resveratrol On TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: a Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. Int J Mol Sci. 2020 Feb 20;21(4) PubMed PMID: 32093379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. AU - Martin,Lynn M, AU - Johnson,Philip J, AU - Amorim,Juliana R, AU - DeClue,Amy E, Y1 - 2020/02/20/ PY - 2019/12/20/received PY - 2020/01/27/revised PY - 2020/02/17/accepted PY - 2020/2/26/entrez PY - 2020/2/26/pubmed PY - 2020/2/26/medline KW - cytokine KW - endotoxin KW - equine KW - inflammation KW - lipopolysaccharide KW - nutraceutical KW - resveratrol KW - sepsis KW - supplement JF - International journal of molecular sciences JO - Int J Mol Sci VL - 21 IS - 4 N2 - Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer's recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/32093379/Effects_of_Orally_Administered_Resveratrol_on_TNF,_IL-1β,_Leukocyte_Phagocytic_Activity_and_Oxidative_Burst_Function_in_Horses:_A_Prospective,_Randomized,_Double-Blinded,_Placebo-Controlled_Study L2 - http://www.mdpi.com/resolver?pii=ijms21041453 DB - PRIME DP - Unbound Medicine ER -
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