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Comparison of Shiga toxin-encoding bacteriophages in highly pathogenic strains of Shiga toxin-producing Escherichia coli O157:H7 in the UK.
Microb Genom. 2020 03; 6(3)MG

Abstract

Over the last 35 years in the UK, the burden of Shiga toxin-producing Escherichia coli (STEC) O157:H7 infection has, during different periods of time, been associated with five different sub-lineages (1983-1995, Ia, I/IIa and I/IIb; 1996-2014, Ic; and 2015-2018, IIb). The acquisition of a stx2a-encoding bacteriophage by these five sub-lineages appears to have coincided with their respective emergences. The Oxford Nanopore Technologies (ONT) system was used to sequence, characterize and compare the stx-encoding prophages harboured by each sub-lineage to investigate the integration of this key virulence factor. The stx2a-encoding prophages from each of the lineages causing clinical disease in the UK were all different, including the two UK sub-lineages (Ia and I/IIa) circulating concurrently and causing severe disease in the early 1980s. Comparisons between the stx2a-encoding prophage in sub-lineages I/IIb and IIb revealed similarity to the prophage commonly found to encode stx2c, and the same site of bacteriophage integration (sbcB) as stx2c-encoding prophage. These data suggest independent acquisition of previously unobserved stx2a-encoding phage is more likely to have contributed to the emergence of STEC O157:H7 sub-lineages in the UK than intra-UK lineage to lineage phage transmission. In contrast, the stx2c-encoding prophage showed a high level of similarity across lineages and time, consistent with the model of stx2c being present in the common ancestor to extant STEC O157:H7 and maintained by vertical inheritance in the majority of the population. Studying the nature of the stx-encoding bacteriophage contributes to our understanding of the emergence of highly pathogenic strains of STEC O157:H7.

Authors+Show Affiliations

Norwich Medical School, University of East Anglia, Norwich, UK.Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush EH25 9RG, UK. National Infection Service, Public Health England, London NW9 5EQ, UK.Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush EH25 9RG, UK.Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush EH25 9RG, UK. National Infection Service, Public Health England, London NW9 5EQ, UK.National Infection Service, Public Health England, London NW9 5EQ, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32100710

Citation

Yara, Daniel A., et al. "Comparison of Shiga Toxin-encoding Bacteriophages in Highly Pathogenic Strains of Shiga Toxin-producing Escherichia Coli O157:H7 in the UK." Microbial Genomics, vol. 6, no. 3, 2020.
Yara DA, Greig DR, Gally DL, et al. Comparison of Shiga toxin-encoding bacteriophages in highly pathogenic strains of Shiga toxin-producing Escherichia coli O157:H7 in the UK. Microb Genom. 2020;6(3).
Yara, D. A., Greig, D. R., Gally, D. L., Dallman, T. J., & Jenkins, C. (2020). Comparison of Shiga toxin-encoding bacteriophages in highly pathogenic strains of Shiga toxin-producing Escherichia coli O157:H7 in the UK. Microbial Genomics, 6(3). https://doi.org/10.1099/mgen.0.000334
Yara DA, et al. Comparison of Shiga Toxin-encoding Bacteriophages in Highly Pathogenic Strains of Shiga Toxin-producing Escherichia Coli O157:H7 in the UK. Microb Genom. 2020;6(3) PubMed PMID: 32100710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Shiga toxin-encoding bacteriophages in highly pathogenic strains of Shiga toxin-producing Escherichia coli O157:H7 in the UK. AU - Yara,Daniel A, AU - Greig,David R, AU - Gally,David L, AU - Dallman,Timothy J, AU - Jenkins,Claire, Y1 - 2020/02/24/ PY - 2020/2/27/pubmed PY - 2020/2/27/medline PY - 2020/2/27/entrez KW - Escherichia coli O157:H7 KW - Shiga toxin KW - bacteriophage KW - whole-genome sequencing JF - Microbial genomics JO - Microb Genom VL - 6 IS - 3 N2 - Over the last 35 years in the UK, the burden of Shiga toxin-producing Escherichia coli (STEC) O157:H7 infection has, during different periods of time, been associated with five different sub-lineages (1983-1995, Ia, I/IIa and I/IIb; 1996-2014, Ic; and 2015-2018, IIb). The acquisition of a stx2a-encoding bacteriophage by these five sub-lineages appears to have coincided with their respective emergences. The Oxford Nanopore Technologies (ONT) system was used to sequence, characterize and compare the stx-encoding prophages harboured by each sub-lineage to investigate the integration of this key virulence factor. The stx2a-encoding prophages from each of the lineages causing clinical disease in the UK were all different, including the two UK sub-lineages (Ia and I/IIa) circulating concurrently and causing severe disease in the early 1980s. Comparisons between the stx2a-encoding prophage in sub-lineages I/IIb and IIb revealed similarity to the prophage commonly found to encode stx2c, and the same site of bacteriophage integration (sbcB) as stx2c-encoding prophage. These data suggest independent acquisition of previously unobserved stx2a-encoding phage is more likely to have contributed to the emergence of STEC O157:H7 sub-lineages in the UK than intra-UK lineage to lineage phage transmission. In contrast, the stx2c-encoding prophage showed a high level of similarity across lineages and time, consistent with the model of stx2c being present in the common ancestor to extant STEC O157:H7 and maintained by vertical inheritance in the majority of the population. Studying the nature of the stx-encoding bacteriophage contributes to our understanding of the emergence of highly pathogenic strains of STEC O157:H7. SN - 2057-5858 UR - https://www.unboundmedicine.com/medline/citation/32100710/Comparison_of_Shiga_toxin_encoding_bacteriophages_in_highly_pathogenic_strains_of_Shiga_toxin_producing_Escherichia_coli_O157:H7_in_the_UK_ L2 - http://mgen.microbiologyresearch.org/pubmed/content/journal/mgen/10.1099/mgen.0.000334 DB - PRIME DP - Unbound Medicine ER -