Tags

Type your tag names separated by a space and hit enter

Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation.
Sci Rep. 2020 02 26; 10(1):3544.SR

Abstract

Bacillus licheniformis BL1 was used as a starting strain to construct the recombinant tetramethylpyrazine (TMP)-producing strains by over-expression of the α-acetolactate decarboxylase gene (aldC) and α-acetolactate synthase gene (alsS), named BLC, BLS and BLCS, respectively. Then the addition of acetaldehyde was use to enhance the TMP yield in the fermentation process. During microaerobic fermentation, the aldC-overexpressed BLC strain produced 43.75 g TMP/L which was 15.47% higher than the TMP in culture yielded using the initial BL1 strain. Furthermore, the acetoin yield as TMP precursor similarly rose by 23.06% in BLC recombinant strain. In contrast, the 2,3-BD increased by 23.2% in the recombinant BLCS. TMP produced by BL1 could be bolstered via the supplementation of the acetaldehyde in fermentation medium. This method also has the same effect on the BLC strain.

Authors+Show Affiliations

State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education, Shandong Province, Qilu University of Technology, Jinan, Shandong, 250353, People's Republic of China.State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China.State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. ruiming3k@163.com. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. ruiming3k@163.com.State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. wangtengfei1981@163.com. Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250353, People's Republic of China. wangtengfei1981@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32103037

Citation

Meng, Wu, et al. "Enhanced Production of Tetramethylpyrazine in Bacillus Licheniformis BL1 Through aldC Over-expression and Acetaldehyde Supplementation." Scientific Reports, vol. 10, no. 1, 2020, p. 3544.
Meng W, Ding F, Wang RM, et al. Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation. Sci Rep. 2020;10(1):3544.
Meng, W., Ding, F., Wang, R. M., & Wang, T. F. (2020). Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation. Scientific Reports, 10(1), 3544. https://doi.org/10.1038/s41598-020-60345-3
Meng W, et al. Enhanced Production of Tetramethylpyrazine in Bacillus Licheniformis BL1 Through aldC Over-expression and Acetaldehyde Supplementation. Sci Rep. 2020 02 26;10(1):3544. PubMed PMID: 32103037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced Production of Tetramethylpyrazine in Bacillus licheniformis BL1 through aldC Over-expression and acetaldehyde Supplementation. AU - Meng,Wu, AU - Ding,Feng, AU - Wang,Rui-Ming, AU - Wang,Teng-Fei, Y1 - 2020/02/26/ PY - 2019/03/29/received PY - 2020/02/06/accepted PY - 2020/2/28/entrez PY - 2020/2/28/pubmed PY - 2020/2/28/medline SP - 3544 EP - 3544 JF - Scientific reports JO - Sci Rep VL - 10 IS - 1 N2 - Bacillus licheniformis BL1 was used as a starting strain to construct the recombinant tetramethylpyrazine (TMP)-producing strains by over-expression of the α-acetolactate decarboxylase gene (aldC) and α-acetolactate synthase gene (alsS), named BLC, BLS and BLCS, respectively. Then the addition of acetaldehyde was use to enhance the TMP yield in the fermentation process. During microaerobic fermentation, the aldC-overexpressed BLC strain produced 43.75 g TMP/L which was 15.47% higher than the TMP in culture yielded using the initial BL1 strain. Furthermore, the acetoin yield as TMP precursor similarly rose by 23.06% in BLC recombinant strain. In contrast, the 2,3-BD increased by 23.2% in the recombinant BLCS. TMP produced by BL1 could be bolstered via the supplementation of the acetaldehyde in fermentation medium. This method also has the same effect on the BLC strain. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/32103037/Enhanced_Production_of_Tetramethylpyrazine_in_Bacillus_licheniformis_BL1_through_aldC_Over-expression_and_acetaldehyde_Supplementation L2 - http://dx.doi.org/10.1038/s41598-020-60345-3 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.